LOCUS AH005273 12223 bp DNA linear HUM 01-AUG-2016
DEFINITION Homo sapiens immunoglobulin gamma-2 heavy chain (IgH),
immunoglobulin gamma-4 heavy chain (IgH), immunoglobulin epsilon
chain constant region (IgH), and immunoglobulin alpha-2 heavy chain
(IgH) genes, partial cds.
ACCESSION AH005273 J00221 J00222 J00230 K01316 K01317 K01318 K01319 V00554
VERSION AH005273.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 2110 to 4137)
AUTHORS Ellison,J., Buxbaum,J. and Hood,L.
TITLE Nucleotide sequence of a human immunoglobulin C gamma 4 gene
JOURNAL DNA 1 (1), 11-18 (1981)
PUBMED 6299662
REFERENCE 2 (bases 6219 to 9607)
AUTHORS Ueda,S., Nakai,S., Nishida,Y., Hisajima,H. and Honjo,T.
TITLE Long terminal repeat-like elements flank a human immunoglobulin
epsilon pseudogene that lacks introns
JOURNAL EMBO J. 1 (12), 1539-1544 (1982)
PUBMED 6327276
REFERENCE 3 (bases 896 to 1256; 1749 to 1937; 3003 to 3215)
AUTHORS Krawinkel,U. and Rabbitts,T.H.
TITLE Comparison of the hinge-coding segments in human immunoglobulin
gamma heavy chain genes and the linkage of the gamma 2 and gamma 4
subclass genes
JOURNAL EMBO J. 1 (4), 403-407 (1982)
PUBMED 6329676
REFERENCE 4 (bases 1 to 12223)
AUTHORS Ellison,J. and Hood,L.
TITLE Linkage and sequence homology of two human immunoglobulin gamma
heavy chain constant region genes
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 79 (6), 1984-1988 (1982)
PUBMED 6804948
REFERENCE 5 (bases 7330 to 9326)
AUTHORS Max,E.E., Battey,J., Ney,R., Kirsch,I.R. and Leder,P.
TITLE Duplication and deletion in the human immunoglobulin epsilon genes
JOURNAL Cell 29 (2), 691-699 (1982)
PUBMED 6288268
REFERENCE 6 (bases 475 to 1071; 1179 to 1330; 1461 to 1524; 2584 to 3178;
3289 to 3440; 3541 to 3764)
AUTHORS Takahashi,N., Ueda,S., Obata,M., Nikaido,T., Nakai,S. and Honjo,T.
TITLE Structure of human immunoglobulin gamma genes: implications for
evolution of a gene family
JOURNAL Cell 29 (2), 671-679 (1982)
PUBMED 6811139
REFERENCE 7 (bases 10444 to 10723)
AUTHORS Flanagan,J.G. and Rabbitts,T.H.
TITLE Arrangement of human immunoglobulin heavy chain constant region
genes implies evolutionary duplication of a segment containing
gamma, epsilon and alpha genes
JOURNAL Nature 300 (5894), 709-713 (1982)
PUBMED 6817141
REFERENCE 8 (bases 4238 to 4998; 5099 to 5374; 5475 to 6118; 8250 to 9606;
9756 to 9936; 10132 to 10221)
AUTHORS Hisajima,H., Nishida,Y., Nakai,S., Takahashi,N., Ueda,S. and
Honjo,T.
TITLE Structure of the human immunoglobulin C epsilon 2 gene, a truncated
pseudogene: implications for its evolutionary origin
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (10), 2995-2999 (1983)
PUBMED 6407005
REFERENCE 9 (bases 9708 to 12223)
AUTHORS Flanagan,J.G., Lefranc,M.P. and Rabbitts,T.H.
TITLE Mechanisms of divergence and convergence of the human
immunoglobulin alpha 1 and alpha 2 constant region gene sequences
JOURNAL Cell 36 (3), 681-688 (1984)
PUBMED 6421489
COMMENT On or before Aug 1, 2016 this sequence version replaced J00230.1,
K01316.1, K01317.1, K01318.1, K01319.1, J00222.1, J00221.1,
AH005273.1.
FEATURES Location/Qualifiers
source 1..12223
/organism="Homo sapiens"
/mol_type="genomic DNA"
/db_xref="taxon:9606"
/map="14q32.33"
/germline
gene <1..2009
/gene="IgH"
/note="IGHG2"
intron <1..215
/gene="IgH"
CDS join(<216..509,902..937,1056..1382,1480..1802)
/gene="IgH"
/codon_start=3
/product="immunoglobulin gamma-2 heavy chain"
/protein_id="AAB59393.1"
/translation="STKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGA
LTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKCC
VECPPCPAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFNWYVDGV
EVHNAKTKPREEQFNSTFRVVSVLTVVHQDWLNGKEYKCKVSNKGLPAPIEKTISKTK
GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPML
DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK"
exon 216..509
/gene="IgH"
/note="G00-119-338"
C_region 216..508
/gene="IgH"
/note="immunoglobulin heavy chain constant region CH1"
intron 510..901
/gene="IgH"
misc_difference 537
/gene="IgH"
/note="conflict"
/citation=[6]
/replace=""
misc_difference 550..551
/gene="IgH"
/note="conflict"
/citation=[6]
/replace=""
misc_difference 570
/gene="IgH"
/note="conflict"
/citation=[6]
/replace=""
misc_difference 777..778
/gene="IgH"
/note="conflict"
/citation=[6]
/replace=""
misc_difference 791
/gene="IgH"
/note="conflict"
/citation=[6]
/replace=""
misc_difference 864
/gene="IgH"
/note="conflict"
/citation=[6]
/replace=""
C_region 901..936
/gene="IgH"
/note="immunoglobulin heavy chain hinge"
exon 902..937
/gene="IgH"
/note="G00-119-338"
intron 938..1055
/gene="IgH"
C_region 1055..1381
/gene="IgH"
/note="immunoglobulin heavy chain constant region CH2"
exon 1056..1382
/gene="IgH"
/note="G00-119-338"
intron 1383..1479
/gene="IgH"
C_region 1479..1799
/gene="IgH"
/note="immunoglobulin heavy chain constant region CH3"
exon 1480..1802
/gene="IgH"
/note="G00-119-338"
misc_difference 1493
/gene="IgH"
/note="conflict"
/citation=[6]
/replace=""
misc_difference 1802..1806
/gene="IgH"
/note="conflict"
/citation=[3]
/replace=""
misc_difference 1814..1815
/gene="IgH"
/note="conflict"
/citation=[3]
/replace=""
misc_difference 1825
/gene="IgH"
/note="conflict"
/citation=[3]
/replace=""
misc_difference 1844..1853
/gene="IgH"
/note="conflict"
/citation=[3]
/replace=""
misc_difference 1890
/gene="IgH"
/note="conflict"
/citation=[3]
/replace=""
regulatory 1903..1908
/regulatory_class="polyA_signal_sequence"
/gene="IgH"
misc_difference 1909..1918
/gene="IgH"
/note="conflict"
/citation=[3]
/replace=""
misc_difference 1929..1932
/gene="IgH"
/note="conflict"
/citation=[3]
/replace=""
gap 2010..2109
/estimated_length=unknown
gene <2110..4137
/gene="IgH"
/note="IGHG4"
intron <2110..2324
/gene="IgH"
/note="gamma-4 intron J-C"
CDS join(<2325..2618,3009..3044,3163..3492,3590..3912)
/gene="IgH"
/codon_start=3
/product="immunoglobulin gamma-4 heavy chain"
/protein_id="AAB59394.1"
/translation="STKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGA
LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYG
PPCPSCPAPEFLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSQEDPEVQFNWYVDG
VEVHNAKTKPREEQFNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKA
KGQPREPQVYTLPPSQEEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPV
LDSDGSFFLYSRLTVDKSRWQEGNVFSCSVMHEALHNHYTQKSLSLSLGK"
exon 2325..2618
/gene="IgH"
/note="G00-119-340"
intron 2619..3008
/gene="IgH"
exon 3009..3044
/gene="IgH"
intron 3045..3162
/gene="IgH"
exon 3163..3492
/gene="IgH"
intron 3493..3589
/gene="IgH"
exon 3590..3912
/gene="IgH"
gap 4138..4237
/estimated_length=unknown
gene 4238..6118
/gene="IgH"
/note="IGHEP1"
intron <4238..>4998
/gene="IgH"
/note="epsilon-1 intron J-C; G00-119-336"
gap 4999..5098
/estimated_length=unknown
intron <5099..>5374
/gene="IgH"
/note="epsilon-1 intron J-C; G00-119-336"
gap 5375..5474
/estimated_length=unknown
intron <5475..>6118
/gene="IgH"
/note="epsilon-1 intron J-C; G00-119-336"
gap 6119..6218
/estimated_length=unknown
gene <6219..9090
/gene="IgH"
/note="IGHE"
intron <6219..7427
/gene="IgH"
misc_difference 7364
/gene="IgH"
/note="conflict"
/citation=[5]
/replace=""
misc_difference 7373
/gene="IgH"
/note="conflict"
/citation=[5]
/replace=""
CDS join(<7428..7736,7944..8264,8351..8674,8758..9089)
/gene="IgH"
/codon_start=3
/product="immunoglobulin epsilon chain constant region"
/protein_id="AAB59395.1"
/translation="STQSPSVFPLTRCCKNIPSNATSVTLGCLATGYFPEPVMVTCDT
GSLNGTTMTLPATTLTLSGHYATISLLTVSGAWAKQMFTCRVAHTPSSTDWVDNKTFS
VCSRDFTPPTVKILQSSCDGGGHFPPTIQLLCLVSGYTPGTINITWLEDGQVMDVDLS
TASTTQEGELASTQSELTLSQKHWLSDRTYTCQVTYQGHTFEDSTKKCADSNPRGVSA
YLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVNHSTRKEEKQRNGT
LTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWP
GSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVT
RAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGK"
exon 7428..7736
/gene="IgH"
/note="G00-119-335"
misc_difference 7555
/gene="IgH"
/note="conflict"
/citation=[5]
/replace=""
intron 7737..7943
/gene="IgH"
misc_difference 7846..7847
/gene="IgH"
/note="conflict"
/citation=[5]
/replace=""
misc_difference 7936
/gene="IgH"
/note="conflict"
/citation=[5]
/replace=""
exon 7944..8264
/gene="IgH"
intron 8265..8350
/gene="IgH"
exon 8351..8674
/gene="IgH"
misc_difference 8569
/gene="IgH"
/note="conflict"
/citation=[8]
/replace=""
misc_difference 8634
/gene="IgH"
/note="conflict"
/citation=[8]
/replace=""
intron 8675..8757
/gene="IgH"
exon 8758..9090
/gene="IgH"
misc_difference 8878
/gene="IgH"
/note="conflict"
/citation=[5]
/replace=""
misc_difference 8900
/gene="IgH"
/note="conflict"
/citation=[5]
/replace=""
misc_difference 8972
/gene="IgH"
/note="conflict"
/citation=[5]
/replace=""
misc_difference 9220..9224
/note="conflict"
/citation=[8]
/replace=""
misc_difference 9225..9226
/note="conflict"
/citation=[5]
/replace=""
misc_difference 9284..9289
/note="conflict"
/citation=[5]
/replace=""
misc_difference 9325
/note="conflict"
/citation=[5]
/replace=""
gap 9608..9707
/estimated_length=unknown
gene <9708..11328
/gene="IgH"
/note="IGHA2"
intron <9708..9870
/gene="IgH"
/note="alpha-2 intron J-C"
CDS join(<9871..10176,10391..10711,10934..11328)
/gene="IgH"
/note="contains constant region"
/codon_start=3
/product="immunoglobulin alpha-2 heavy chain"
/protein_id="AAB59396.1"
/translation="SPTSPKVFPLSLDSTPQDGNVVVACLVQGFFPQEPLSVTWSESG
QNVTARNFPPSQDASGDLYTTSSQLTLPATQCPDGKSVTCHVKHYTNPSQDVTVPCPV
PPPPPCCHPRLSLHRPALEDLLLGSEANLTCTLTGLRDASGATFTWTPSSGKSAVQGP
PERDLCGCYSVSSVLPGCAQPWNHGETFTCTAAHPELKTPLTANITKSGNTFRPEVHL
LPPPSEELALNELVTLTCLARGFSPKDVLVRWLQGSQELPREKYLTWASRQEPSQGTT
TFAVTSILRVAAEDWKKGDTFSCMVGHEALPLAFTQKTIDRLAGKPTHVNVSVVMAEV
DGTCY"
exon 9871..10176
/gene="IgH"
/note="G00-119-333"
intron 10177..10390
/gene="IgH"
/note="alpha-2 intron A"
exon 10391..10711
/gene="IgH"
intron 10712..10933
/gene="IgH"
/note="alpha-2 intron B"
exon 10934..11328
/gene="IgH"
variation 11141
/gene="IgH"
/note="t in A2m(1); a in A2m(2)"
variation 11148
/gene="IgH"
/note="g in A2m(1); a in A2m(2)"
variation 11172
/gene="IgH"
/note="c in A2m(1); t in A2m(2)"
variation 11193
/gene="IgH"
/note="c in A2m(1); g in A2m(2)"
variation 11260
/gene="IgH"
/note="t in A2m(1); a in A2m(2)"
variation 11280..11281
/gene="IgH"
/note="tg in A2m(1); ca in A2m(2)"
variation 11309..11313
/gene="IgH"
/note="tggac in A2m(1); cggat in A2m(2)"
variation 11767
/note="c in A2m(1); t in A2m(2)"
variation 12091
/note="a in A2m(1); c in A2m(2)"
variation 12097
/note="c in A2m(1); g in A2m(2)"
BASE COUNT 2378 a 3688 c 3507 g 2014 t
ORIGIN
1 agctttctgg ggcgagccgg gcctgacttt ggctttgggg cagggagtgg gctaaggtga
61 ggcaggtggc gccagccagg tgcacaccca atgcccgtga gcccagacac tggaccctgc
121 ctggaccctc gtggatagac aagaaccgag gggcctctgc gcctgggccc agctctgtcc
181 cacaccgcgg tcacatggca ccacctctct tgcagcctcc accaagggcc catcggtctt
241 ccccctggcg ccctgctcca ggagcacctc cgagagcaca gccgccctgg gctgcctggt
301 caaggactac ttccccgaac cggtgacggt gtcgtggaac tcaggcgctc tgaccagcgg
361 cgtgcacacc ttcccagctg tcctacagtc ctcaggactc tactccctca gcagcgtggt
421 gaccgtgccc tccagcaact tcggcaccca gacctacacc tgcaacgtag atcacaagcc
481 cagcaacacc aaggtggaca agacagttgg tgagaggcca gctcagggag ggagggtgtc
541 tgctggaagc caggctcagc cctcctgcct ggacgcaccc cggctgtgca gccccagccc
601 agggcagcaa ggcaggcccc atctgtctcc tcacccggag gcctctgccc gccccactca
661 tgctcaggga gagggtcttc tggctttttc caccaggctc caggcaggca caggctgggt
721 gcccctaccc caggcccttc acacacaggg gcaggtgctt ggctcagacc tgccaaaagc
781 catatccggg aggaccctgc ccctgaccta agccgacccc aaaggccaaa ctgtccactc
841 cctcagctcg gacaccttct ctcctcccag atccgagtaa ctcccaatct tctctctgca
901 gagcgcaaat gttgtgtcga gtgcccaccg tgcccaggta agccagccca ggcctcgccc
961 tccagctcaa ggcgggacag gtgccctaga gtagcctgca tccagggaca ggccccagct
1021 gggtgctgac acgtccacct ccatctcttc ctcagcacca cctgtggcag gaccgtcagt
1081 cttcctcttc cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac
1141 gtgcgtggtg gtggacgtga gccacgaaga ccccgaggtc cagttcaact ggtacgtgga
1201 cggcgtggag gtgcataatg ccaagacaaa gccacgggag gagcagttca acagcacgtt
1261 ccgtgtggtc agcgtcctca ccgttgtgca ccaggactgg ctgaacggca aggagtacaa
1321 gtgcaaggtc tccaacaaag gcctcccagc ccccatcgag aaaaccatct ccaaaaccaa
1381 aggtgggacc cgcggggtat gagggccaca tggacagagg ccggctcggc ccaccctctg
1441 ccctgggagt gaccgctgtg ccaacctctg tccctacagg gcagccccga gaaccacagg
1501 tgtacaccct gcccccatcc cgggaggaga tgaccaagaa ccaggtcagc ctgacctgcc
1561 tggtcaaagg cttctacccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg
1621 agaacaacta caagaccaca cctcccatgc tggactccga cggctccttc ttcctctaca
1681 gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga
1741 tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaat
1801 gagtgccacg gccggcaagc ccccgctccc caggctctcg gggtcgcgtg aggatgcttg
1861 gcacgtaccc cgtgtacata cttcccaggc acccagcatg gaaataaagc acccagcgct
1921 gccctgggcc cctgcgagac tgtgatggtt ctttccgtgg gtcaggccga gtctgaggcc
1981 tgagtggcat gagggaggca gagtgggtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
2101 nnnnnnnnna gctttctggg gcaggccggg cctgactttg gctgggggca gggagggggc
2161 taaggtgacg caggtggcgc cagccaggtg cacacccaat gcccatgagc ccagacactg
2221 gaccctgcat ggaccatcgc ggatagacaa gaaccgaggg gcctctgcgc cctgggccca
2281 gctctgtccc acaccgcggt cacatggcac cacctctctt gcagcttcca ccaagggccc
2341 atccgtcttc cccctggcgc cctgctccag gagcacctcc gagagcacag ccgccctggg
2401 ctgcctggtc aaggactact tccccgaacc ggtgacggtg tcgtggaact caggcgccct
2461 gaccagcggc gtgcacacct tcccggctgt cctacagtcc tcaggactct actccctcag
2521 cagcgtggtg accgtgccct ccagcagctt gggcacgaag acctacacct gcaacgtaga
2581 tcacaagccc agcaacacca aggtggacaa gagagttggt gagaggccag cacagggagg
2641 gagggtgtct gctggaagcc aggctcagcc ctcctgcctg gacgcacccc ggctgtgcag
2701 ccccagccca gggcagcaag gcatgcccca tctgtctcct cacccggagg cctctgacca
2761 ccccactcat gctcagggag agggtcttct ggatttttcc accaggctcc cggcaccaca
2821 ggctggatgc ccctacccca ggccctgcgc atacagggca ggtgctgcgc tcagacctgc
2881 caagagccat atccgggagg accctgcccc tgacctaagc ccaccccaaa ggccaaactc
2941 tccactccct cagctcagac accttctctc ctcccagatc tgagtaactc ccaatcttct
3001 ctctgcagag tccaaatatg gtcccccatg cccatcatgc ccaggtaagc caacccaggc
3061 ctcgccctcc agctcaaggc gggacaggtg ccctagagta gcctgcatcc agggacaggc
3121 cccagccggg tgctgacgca tccacctcca tctcttcctc agcacctgag ttcctggggg
3181 gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc tcccggaccc
3241 ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc cagttcaact
3301 ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag gagcagttca
3361 acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg ctgaacggca
3421 aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag aaaaccatct
3481 ccaaagccaa aggtgggacc cacggggtgc gagggccaca cggacagagg ccagctcggc
3541 ccaccctctg ccctgggagt gaccgctgtg ccaacctctg tccctacagg gcagccccga
3601 gagccacagg tgtacaccct gcccccatcc caggaggaga tgaccaagaa ccaggtcagc
3661 ctgacctgcc tggtcaaagg cttctacccc agcgacatcg ccgtggagtg ggagagcaat
3721 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc
3781 ttcctctaca gcaggctaac cgtggacaag agcaggtggc aggaggggaa tgtcttctca
3841 tgctccgtga tgcatgaggc tctgcacaac cactacacac agaagagcct ctccctgtct
3901 ctgggtaaat gagtgccagg gccggcaagc ccccgctccc cgggctctcg gggtcgcgcg
3961 aggatgcttg gcacgtaccc cgtctacata cttcccaggc acccagcatg gaaataaagc
4021 acccaccact gccctgggcc cctgtgagac tgtgatggtt ctttccacgg gtcaggccga
4081 gtctgaggcc tgagtgacat gagggaggca gagcgggtcc cactgtcccc acactggnnn
4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct tccacatttt caggtatcct
4261 tatagcaatg ttccactcct caataccaat gttctgagtt agtctatttg tgttactaga
4321 aaaaaaaaaa acatgaatct ggtaatttat aaagaaaaga ggtttgcacc aggcactgtg
4381 gcaaggaagg gaggattaac tagctcacag ttctgcagcc attccggaaa cacggccctg
4441 gcatctgctt ctggcgaggc ctcagggaga ttgcaatcac ggtagaaggc gaagggggag
4501 caggcatgtc acatggcaag agggagcaag agaagagaag tggcacgtcc cagactttta
4561 aacaatgagc tgtcacaggg actaactgag ggagaactcg ctcatcacct gggagatggt
4621 gctaggccat tcatgagggg tccagcccca tgatctaatc acctcccacn annnncacct
4681 ccaacactgg gaatcacatc tcaacatgaa atttggagag acacacatcc aaactattgc
4741 accacaggaa gctggaagag gtggacggtc ctcccctgga gcactcagag acagccacca
4801 acaccttgnt ctcagattcc tgactcccag aacnctgaaa anatttttgt tgttttaagc
4861 cacatnnnna tattttgtta cggcagccac agcacgnnnn nnnntggcag agttctttag
4921 cctccnnntg cancctgggg taaataacta ttgaatttgg ccacatggaa atcaggaaga
4981 atgtaataag catcatgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
5101 cccaggcaac gggaccccaa gtttgctgac tgggaccacc aagcccnntg cgtcaagagt
5161 gagagtccgg gacctaggca ggggccctgg ggttgggcct ggggggaccn tcccccagtc
5221 actcggtgtg catcggtagt gaaggagcct cacctgaccc ccgctgttgc tcaatcgact
5281 tcccaagaac agagagaaaa gggaacttcc aaggcggccc gggcctccgg gggttcccac
5341 cccattttta gctgaaagca ctgaggcaga gctcnnnnnn nnnnnnnnnn nnnnnnnnnn
5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
5461 nnnnnnnnnn nnnnagctta gctggttggg ctgagtaagc tgggctgagc taaatgggat
5521 tgagctgagg agggctaggc tgggggagag acctgacgac ggacagggtt aaaagctgga
5581 gtgagaggcc ttaaattatt gaactaaatt gggctggggt gatctgaatt tagctgggat
5641 gagctgggnn nnnnngggct gtgcccacgc gagctgggct aagctaggct cgcctgagtg
5701 gactcagctg ggttggtctc aactactggg ttcagctggg ctgggctgag ctaggttaga
5761 ctggggaagg ctgggctggg tcgggctggg ttgggctggg ctgctcaagg ctaagctaac
5821 ctaacctaac ctgggttcag ctggcctagg ttgggctggg ttgggctaag ctgggttcag
5881 ctgagatatg ctaatatggg ctgggctggg tcaggttgag gttaactgaa ctgggctgac
5941 ctgggctgag ctcaactgag ttcacatggg ctgggctggc ctggcctggc ctaaactggg
6001 tttggctggg ctgggccaac tggactgagg tggatggagc tgggctgagc tggcctggcc
6061 gggcctcagc tgtgattgga agacctgggc tggagctgga cagacctgag ccaagcttnn
6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tggactgggc tgagctagac
6241 tgggcaactg tattcagctg ggctggcctg gcctcggctg ggctgggttc agctgggctg
6301 ggctgagcag gcctgagcag gattagttga gctggtcgta actggattta actagctagg
6361 ctaggcttaa ctgactaagc tgatctggac tgtattcatc tggatgaagc tggggtgagg
6421 tggctacttt aggtcagctt tgctgagcta aactggaccg ggctaaattg atctggactg
6481 accattctca cctggctaag aggagctgag tcagaagcaa gctggttgag ctggctggac
6541 tgaaataaga gtttgctgcc tgcaagggga ggtcctgggc tgacctgggc caggctgaac
6601 caggctggct tagagtgaac ttcagagggc gactcccccg gtaggccagt ctcagctgaa
6661 cttggctgtc ccggtgggca gagcggggct ggatactgtg attttggggg tacctagagc
6721 agacttcaag accaagctaa actgggctcc aggggcagga tgggctgggg acttgggact
6781 ccaggccagg ggcgaagggc cacgctgtac agaccgcact atctgggcca gggttctgtg
6841 gtgggaggga ctgactgcct ggggcatcag ggcaagtctt cccgccctcc cctagaggtc
6901 aggggtgggc agagcaccat gggggtctgg caggtcaggt gagggctgct gtgatgggga
6961 gatccaggct tggcactcaa gagcccgagg agctgagacc acagccttgg ggggttgggg
7021 tcagggttgg agggcaggca gaccatccac catgagccca gagagagttt gaagggggag
7081 ggctctgggg tccggcccat ggggtccctg ggtttcagcc taggggcatg gcccagtgtc
7141 tctgctcctg agtgcccacc gtgcagcact tgcaggggga ggctggggtc atcctggagg
7201 cacccccctt cctgagccca gcctgatgat agtggctgag caacagcttc tggtggggga
7261 atgggccctg ggagccgccc tgggcctggg gattgtgggg aaaaaggccc agaatgagcc
7321 tagccatctg gatccctgcc acggggtccc cagctccccc atccaggccc cccaggcctg
7381 atgggcgctg gcctgaggct ggcactgact aggttctgtc ctcacagcct ccacacagag
7441 cccatccgtc ttccccttga cccgctgctg caaaaacatt ccctccaatg ccacctccgt
7501 gactctgggc tgcctggcca cgggctactt cccggagccg gtgatggtga cctgcgacac
7561 aggctccctc aacgggacaa ctatgacctt accagccacc accctcacgc tctctggtca
7621 ctatgccacc atcagcttgc tgaccgtctc gggtgcgtgg gccaagcaga tgttcacctg
7681 ccgtgtggca cacactccat cgtccacaga ctgggtcgac aacaaaacct tcagcggtaa
7741 gagagggcca agctcagaga ccacagttcc caggagtgcc aggctgaggg ctggcagagt
7801 gggcaggggt tgagggggtg ggtgggctca aacgtgggaa cacccagcat gcctggggac
7861 ccgggccagg acgtgggggc aagaggaggg cacacagagc tcagagaggc caacaaccct
7921 catgaccacc agctctcccc cagtctgctc cagggacttc accccgccca ccgtgaagat
7981 cttacagtcg tcctgcgacg gcggcgggca cttccccccg accatccagc tcctgtgcct
8041 cgtctctggg tacaccccag ggactatcaa catcacctgg ctggaggacg ggcaggtcat
8101 ggacgtggac ttgtccaccg cctctaccac gcaggagggt gagctggcct ccacacaaag
8161 cgagctcacc ctcagccaga agcactggct gtcagaccgc acctacacct gccaggtcac
8221 ctatcaaggt cacacctttg aggacagcac caagaagtgt gcaggtacgt tcccacctgc
8281 cctggtggcc gccacggagg ccagagaaga ggggcgggtg ggcctcacac agccctccgg
8341 tgtaccacag attccaaccc gagaggggtg agcgcctacc taagccggcc cagcccgttc
8401 gacctgttca tccgcaagtc gcccacgatc acctgtctgg tggtggacct ggcacccagc
8461 aaggggaccg tgaacctgac ctggtcccgg gccagtggga agcctgtgaa ccactccacc
8521 agaaaggagg agaagcagcg caatggcacg ttaaccgtca cgtccaccct gccggtgggc
8581 acccgagact ggatcgaggg ggagacctac cagtgcaggg tgacccaccc ccacctgccc
8641 agggccctca tgcggtccac gaccaagacc agcggtgagc catgggcagg ccggggtcgt
8701 gggggaaggg agggagcgag tgagcggggc ccgggctgac cccacgtctg gccacaggcc
8761 cgcgtgctgc cccggaagtc tatgcgtttg cgacgccgga gtggccgggg agccgggaca
8821 agcgcaccct cgcctgcctg atccagaact tcatgcctga ggacatctcg gtgcagtggc
8881 tgcacaacga ggtgcagctc ccggacgccc ggcacagcac gacgcagccc cgcaagacca
8941 agggctccgg cttcttcgtc ttcagccgcc tggaggtgac cagggccgaa tgggagcaga
9001 aagatgagtt catctgccgt gcagtccatg aggcagcgag cccctcacag accgtccagc
9061 gagcggtgtc tgtaaatccc ggtaaatgac gtactcctgc ctccctccct cccagggctc
9121 catccagctg tgcagtgggg aggactggcc agaccttctg tccactgttg caatgacccc
9181 aggaagctac ccccaataaa ctgtgcctgc tcagagcccc aggtacaccc attcttggga
9241 gcgggcaggg ctgtgggcag gtgcatcttg gcacagagga atggccccca ggaggggcag
9301 tgggaggagg tgggcagggc tgagcccccc ctggagaggc ggtgggagga ggtgggcagg
9361 gctgaggtgc cactcatcca tctgccttcg tgtcagggtt atttgtcaaa cagcatatct
9421 gcagggactc atcacagcta ccccgggccc tctctgcccc cactctgggt ctaccccctc
9481 caaggagtcc aaagacccag gggaggtcct cagggaaggg gcaagggagc ccacagccct
9541 ctctcttggg ggcttggctt ctacccccct ggacaggagc ccctgcaccc ccaggtatag
9601 atgggcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt ccaaccgcag
9721 gcccatggtg caggagctgt gtaacctatg gggctgtcac caggcctctc tgtgctgggt
9781 tcctccagtg tagaggagag gcaggtacag cctgtcctcc tggggacatg gcatgagggc
9841 cgcgtcctca cagcgcattc tgtgttccag catccccgac cagccccaag gtcttcccgc
9901 tgagcctcga cagcaccccc caagatggga acgtggtcgt cgcatgcctg gtccagggct
9961 tcttccccca ggagccactc agtgtgacct ggagcgaaag cggacagaac gtgaccgcca
10021 gaaacttccc acctagccag gatgcctccg gggacctgta caccacgagc agccagctga
10081 ccctgccggc cacacagtgc ccagacggca agtccgtgac atgccacgtg aagcactaca
10141 cgaatcccag ccaggatgtg actgtgccct gcccaggtca gagggcaggc tggggagtgg
10201 ggcggggcca ccccgtcctg ccctgacact gcgcctgcac ccgtgttccc cacagggagc
10261 cgccccttca ctcacaccag agtggacccc gggccgagcc ccaggaggtg gtggtggaca
10321 ggccaggagg ggcgaggcgg gggcacgggg aagggcgttc tgaccagctc aggccatctc
10381 tccactccag ttcccccacc tcccccatgc tgccaccccc gactgtcgct gcaccgaccg
10441 gccctcgagg acctgctctt aggttcagaa gcgaacctca cgtgcacact gaccggcctg
10501 agagatgcct ctggtgccac cttcacctgg acgccctcaa gtgggaagag cgctgttcaa
10561 ggaccacctg agcgtgacct ctgtggctgc tacagcgtgt ccagtgtcct gcctggctgt
10621 gcccagccat ggaaccatgg ggagaccttc acctgcactg ctgcccaccc cgagttgaag
10681 accccactaa ccgccaacat cacaaaatcc ggtgggtcca gaccctgctc ggggccctgc
10741 tcagtgctct ggtttgcaaa gcatattccc ggcctgcctc ctccctccca atcctgggct
10801 ccagtgctca tgccaagtac acagggaaac tgaggcaggc tgaggggcca ggacacagcc
10861 cagggtgccc accagagcag aggggctctc tcatcccctg cccagccccc tgacctggct
10921 ctctaccctc caggaaacac attccggccc gaggtccacc tgctgccgcc gccgtcggag
10981 gagctggccc tgaacgagct ggtgacgctg acgtgcctgg cacgtggctt cagccccaag
11041 gatgtgctgg ttcgctggct gcaggggtca caggagctgc cccgcgagaa gtacctgact
11101 tgggcatccc ggcaggagcc cagccagggc accaccacct tcgctgtgac cagcatactg
11161 cgcgtggcag ccgaggactg gaagaagggg gacaccttct cctgcatggt gggccacgag
11221 gccctgccgc tggccttcac acagaagacc atcgaccgct tggcgggtaa acccacccat
11281 gtcaatgtgt ctgttgtcat ggcggaggtg gacggcacct gctactgagc cgcccgcctg
11341 tccccacccc tgaataaact ccatgctccc ccaagcagcc ccacgcttcc atccggcgcc
11401 tgtctgtcca tcctcagggt ctcagcactt gggaaagggc cagggcatgg acagggaaga
11461 ataccccctg ccctgagcct cggggggccc ctggcacccc catgagactt tccaccctgg
11521 tgtgagtgtg agttgtgagt gtgagagtgt gtggtgcagg aggcctcgct ggtgtgagat
11581 cttaggtctg ccaaggcagg cacagcccag gatgggttct gagagacgca catgccccgg
11641 acagttctga gtgagcagtg gcatggccgt ttgtccctga gagagccgcc tctggctgta
11701 gctgggaggg aatagggagg gtaaaaggag caggctagcc aagaaaggcg caggtagtgg
11761 caggagcggc gagggagtga ggggctggac tccagggccc cactgggagg acaagctcca
11821 ggagggcccc accaccctag tgggtgggcc tcaggacgtc ccactgacgc atgcaggaag
11881 gggcacctcc ccttaaccac actgctctgt acggggcacg tgggcacaca tgcacactca
11941 cactcacata tacgcctgag ccctgcagga gtggaacgtt cacagcccag acccagttcc
12001 agaaaagcca ggggagtccc ctcccaagcc cccaagctca gcctgctccc ccaggcccct
12061 ctggcttccc tgtgtttcca ctgtgcacag atcaggcacc aactccacag acccctccca
12121 ggcagcccct gctccctgcc tggccaagtc tcccatccct tcctaagccc aactaggacc
12181 caaagcatag acagggaggg gccgcgtggg gtggcatcag aag
//