LOCUS GQ890670 3201 bp mRNA linear HUM 25-NOV-2009
DEFINITION Homo sapiens truncated GLI1 (tGLI1) mRNA, complete cds,
alternatively spliced.
ACCESSION GQ890670
VERSION GQ890670.2
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 3201)
AUTHORS Lo,H.W., Zhu,H., Cao,X., Aldrich,A. and Ali-Osman,F.
TITLE A novel splice variant of GLI1 that promotes glioblastoma cell
migration and invasion
JOURNAL Cancer Res. 69 (17), 6790-6798 (2009)
PUBMED 19706761
REFERENCE 2 (bases 1 to 3201)
AUTHORS Lo,H.-W.
TITLE Direct Submission
JOURNAL Submitted (08-SEP-2009) Surgery (Box 3156), Duke University, 433A
MSRB I, 103 Research Drive, Durham, NC 27710, USA
REFERENCE 3 (bases 1 to 3201)
AUTHORS Lo,H.-W.
TITLE Direct Submission
JOURNAL Submitted (25-NOV-2009) Surgery (Box 3156), Duke University, 433A
MSRB I, 103 Research Drive, Durham, NC 27710, USA
REMARK Sequence update by submitter
COMMENT On Nov 25, 2009 this sequence version replaced GQ890670.1.
FEATURES Location/Qualifiers
source 1..3201
/db_xref="H-InvDB:HIT000648378"
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="taxon:9606"
/cell_line="U87MG"
/cell_type="glioblastoma cells"
gene 1..3201
/gene="tGLI1"
CDS 1..3201
/gene="tGLI1"
/note="tGLI1; contains a deletion of 41 amino acids that
spans the entire exon 3 and a part of exon 4; variant
frequently detected in cancer cells; gain-of function
transcription factor that promotes cancer cell migration
and invasion; alternatively spliced"
/codon_start=1
/product="truncated GLI1"
/protein_id="ACX35434.1"
/translation="MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEVKLTKKRALSI
SPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQK
GPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSS
PNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSE
HIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLEN
LKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTD
PSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPE
GAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTG
LSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSI
SSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAAS
SLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHT
PPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMD
FPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWG
EFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSE
GPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQL
VCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPN
LPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGH
PEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSS
GHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSAS"
BASE COUNT 692 a 1039 c 848 g 622 t
ORIGIN
1 atgttcaact cgatgacccc accaccaatc agtagctatg gcgagccctg ctgtctccgg
61 cccctcccca gtcagggggc ccccagtgtg gggacagaag tcaagttgac caagaagcgg
121 gcactgtcca tctcacctct gtcggatgcc agcctggacc tgcagacggt tatccgcacc
181 tcacccagct ccctcgtagc tttcatcaac tcgcgatgca catctccagg aggctcctac
241 ggtcatctct ccattggcac catgagccca tctctgggat tcccagccca gatgaatcac
301 caaaaagggc cctcgccttc ctttggggtc cagccttgtg gtccccatga ctctgcccgg
361 ggtgggatga tcccacatcc tcagtcccgg ggacccttcc caacttgcca gctgaagtct
421 gagctggaca tgctggttgg caagtgccgg gaggaaccct tggaaggtga tatgtccagc
481 cccaactcca caggcataca ggatcccctg ttggggatgc tggatgggcg ggaggacctc
541 gagagagagg agaagcgtga gcctgaatct gtgtatgaaa ctgactgccg ttgggatggc
601 tgcagccagg aatttgactc ccaagagcag ctggtgcacc acatcaacag cgagcacatc
661 cacggggagc ggaaggagtt cgtgtgccac tgggggggct gctccaggga gctgaggccc
721 ttcaaagccc agtacatgct ggtggttcac atgcgcagac acactggcga gaagccacac
781 aagtgcacgt ttgaagggtg ccggaagtca tactcacgcc tcgaaaacct gaagacgcac
841 ctgcggtcac acacgggtga gaagccatac atgtgtgagc acgagggctg cagtaaagcc
901 ttcagcaatg ccagtgaccg agccaagcac cagaatcgga cccattccaa tgagaagccg
961 tatgtatgta agctccctgg ctgcaccaaa cgctatacag atcctagctc gctgcgaaaa
1021 catgtcaaga cagtgcatgg tcctgacgcc catgtgacca aacggcaccg tggggatggc
1081 cccctgcctc gggcaccatc catttctaca gtggagccca agagggagcg ggaaggaggt
1141 cccatcaggg aggaaagcag actgactgtg ccagagggtg ccatgaagcc acagccaagc
1201 cctggggccc agtcatcctg cagcagtgac cactccccgg cagggagtgc agccaataca
1261 gacagtggtg tggaaatgac tggcaatgca gggggcagca ctgaagacct ctccagcttg
1321 gacgagggac cttgcattgc tggcactggt ctgtccactc ttcgccgcct tgagaacctc
1381 aggctggacc agctacatca actccggcca atagggaccc ggggtctcaa actgcccagc
1441 ttgtcccaca ccggtaccac tgtgtcccgc cgcgtgggcc ccccagtctc tcttgaacgc
1501 cgcagcagca gctccagcag catcagctct gcctatactg tcagccgccg ctcctccctg
1561 gcctctcctt tcccccctgg ctccccacca gagaatggag catcctccct gcctggcctt
1621 atgcctgccc agcactacct gcttcgggca agatatgctt cagccagagg gggtggtact
1681 tcgcccactg cagcatccag cctggatcgg ataggtggtc ttcccatgcc tccttggaga
1741 agccgagccg agtatccagg atacaacccc aatgcagggg tcacccggag ggccagtgac
1801 ccagcccagg ctgctgaccg tcctgctcca gctagagtcc agaggttcaa gagcctgggc
1861 tgtgtccata ccccacccac tgtggcaggg ggaggacaga actttgatcc ttacctccca
1921 acctctgtct actcaccaca gccccccagc atcactgaga atgctgccat ggatgctaga
1981 gggctacagg aagagccaga agttgggacc tccatggtgg gcagtggtct gaacccctat
2041 atggacttcc cacctactga tactctggga tatgggggac ctgaaggggc agcagctgag
2101 ccttatggag cgaggggtcc aggctctctg cctcttgggc ctggtccacc caccaactat
2161 ggccccaacc cctgtcccca gcaggcctca tatcctgacc ccacccaaga aacatggggt
2221 gagttccctt cccactctgg gctgtaccca ggccccaagg ctctaggtgg aacctacagc
2281 cagtgtcctc gacttgaaca ttatggacaa gtgcaagtca agccagaaca ggggtgccca
2341 gtggggtctg actccacagg actggcaccc tgcctcaatg cccaccccag tgaggggccc
2401 ccacatccac agcctctctt ttcccattac ccccagccct ctcctcccca atatctccag
2461 tcaggcccct atacccagcc accccctgat tatcttcctt cagaacccag gccttgcctg
2521 gactttgatt cccccaccca ttccacaggg cagctcaagg ctcagcttgt gtgtaattat
2581 gttcaatctc aacaggagct actgtgggag ggtgggggca gggaagatgc ccccgcccag
2641 gaaccttcct accagagtcc caagtttctg gggggttccc aggttagccc aagccgtgct
2701 aaagctccag tgaacacata tggacctggc tttggaccca acttgcccaa tcacaagtca
2761 ggttcctatc ccaccccttc accatgccat gaaaattttg tagtgggggc aaatagggct
2821 tcacataggg cagcagcacc acctcgactt ctgcccccat tgcccacttg ctatgggcct
2881 ctcaaagtgg gaggcacaaa ccccagctgt ggtcatcctg aggtgggcag gctaggaggg
2941 ggtcctgcct tgtaccctcc tcccgaagga caggtatgta accccctgga ctctcttgat
3001 cttgacaaca ctcagctgga ctttgtggct attctggatg agccccaggg gctgagtcct
3061 cctccttccc atgatcagcg gggcagctct ggacataccc cacctccctc tgggcccccc
3121 aacatggctg tgggcaacat gagtgtctta ctgagatccc tacctgggga aacagaattc
3181 ctcaactcta gtgcctccta a
//