LOCUS EU921895 1691 bp mRNA linear HUM 27-AUG-2008
DEFINITION Homo sapiens uridine monophosphate synthetase isoform J (UMPS)
mRNA, complete cds.
ACCESSION EU921895
VERSION EU921895.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1691)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Genomic analysis of UMPS expression and sequence reveals novel
isoforms and sequence polymorphisms associated with 5-FU resistance
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1691)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Direct Submission
JOURNAL Submitted (24-JUL-2008) Genome Sciences Centre, British Columbia
Cancer Agency, BC Cancer Research Centre, 675 West 10th Avenue, 7th
Floor, Vancouver, BC V5Z 1L3, Canada
COMMENT Created by TOPO TA cloning. Direction is forward with respect to
M13F site. PCR primers used to generate clone were
5'-CAAACAGGCAGCGCGCGACA-3' (forward) and
5'-GCTGACTTTAGCCTCTTGGTGCCC-3' (reverse).
FEATURES Location/Qualifiers
source 1..1691
/db_xref="H-InvDB:HIT000558359"
/organism="Homo sapiens"
/mol_type="mRNA"
/isolation_source="colorectal cancer cell line"
/db_xref="taxon:9606"
/clone="HCT-5FU_1650_4"
/cell_line="HCT116/5FU"
/PCR_primers="fwd_seq: caaacaggcagcgcgcgaca, rev_seq:
gctgactttagcctcttggtgccc"
gene 1..1691
/gene="UMPS"
/gene_synonym="OPRT"
CDS 401..1018
/gene="UMPS"
/gene_synonym="OPRT"
/codon_start=1
/product="uridine monophosphate synthetase isoform J"
/protein_id="ACH48233.1"
/translation="MLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSIKEAP
KELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPSICML
KTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGDNLGQQYNSP
QEVIGKRGSDIIIVGRGIISAADRLEAAEMYRKAAWEAYLSRLGV"
BASE COUNT 431 a 353 c 433 g 474 t
ORIGIN
1 caaacaggca gcgcgcgaca atggcggtcg ctcgtgcagc tttggggcca ttggtgacgg
61 gtctgtacga cgtgcaggct ttcaagtttg gggacttcgt gctgaagagc gggctttcct
121 cccccatcta catcgatctg cggggcatcg tgtctcgacc gcgtcttctg agtcaggaac
181 taagcgtctt gtagaaggaa ctattaatcc aggagaaacc tgtttaatca ttgaagatgt
241 tgtcaccagt ggatctagtg ttttggaaac tgttgaggtt cttcagaagg agggcttgaa
301 ggtcactgat gccatagtgc tgttggacag agagcaggga ggcaaggaca agttgcaggc
361 gcacgggatc cgcctccact cagtgtgtac attgtccaaa atgctggaga ttctcgagca
421 gcagaaaaaa gttgatgctg agacagttgg gagagtgaag aggtttattc aggagaatgt
481 ctttgtggca gcgaatcata atggttctcc cctttctata aaggaagcac ccaaagaact
541 cagcttcggt gcacgtgcag agctgcccag gatccaccca gttgcatcga agcttctcag
601 gcttatgcaa aagaaggaga ccaatctgtg tctatctgct gatgtttcac tggccagaga
661 gctgttgcag ctagcagatg ctttaggacc tagtatctgc atgctgaaga ctcatgtaga
721 tattttgaat gattttactc tggatgtgat gaaggagttg ataactctgg caaaatgcca
781 tgagttcttg atatttgaag accggaagtt tgcagatata ggaaacacag tgaaaaagca
841 gtatgaagga gataatcttg gccaacagta caatagccca caagaagtta ttggcaaacg
901 aggttccgat atcatcattg taggtcgtgg cataatctca gcagctgatc gtctggaagc
961 agcagagatg tacagaaaag ctgcttggga agcgtatttg agtagacttg gtgtttgagt
1021 gcttcagata catttttcag atacaatgtg aagacattga agatatgtgg tcctcctgaa
1081 agtcactggc tggaaataat ccaattattc ctgcttggat tcttccacag ggcctgtgta
1141 agaatgggtt ctggagttct catggtcttt aggaaatatt gagtaatttg taatcaccgc
1201 attgatacta taataagttc attcttaagc ttgctttttt tgagactggt gtttgttaga
1261 cagccacagt cctgtctggg ttagggtctt ccacatttga ggatccttcc tatctctcca
1321 tgggactaga ctgctttgtt attctattta ttttttaatt tttttcgaga caggatctca
1381 ctctgttgcc caggatggag tgcagtggtg agatcacggc tcattgcagc ctcgacctcc
1441 caggtgatcc tcccacctca gcttccagat tagctggtgc tataggcatg caccaccacg
1501 tccatctaaa tttctttatt atttgtagag atgaggtctt gccatgttac ccaggctggt
1561 ctcaactcct gggctcaagc gatcctcctg cctcagtctc tcaaagtgct gggattacag
1621 gtgtgagcca ctgtgcccag cctaattgca gtaagacaaa aattctaggg caccaagagg
1681 ctaaagtcag c
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