LOCUS EU921893 1789 bp mRNA linear HUM 27-AUG-2008
DEFINITION Homo sapiens uridine monophosphate synthetase isoform H (UMPS)
mRNA, complete cds.
ACCESSION EU921893
VERSION EU921893.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1789)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Genomic analysis of UMPS expression and sequence reveals novel
isoforms and sequence polymorphisms associated with 5-FU resistance
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1789)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Direct Submission
JOURNAL Submitted (24-JUL-2008) Genome Sciences Centre, British Columbia
Cancer Agency, BC Cancer Research Centre, 675 West 10th Avenue, 7th
Floor, Vancouver, BC V5Z 1L3, Canada
COMMENT Created by TOPO TA cloning. Direction is forward with respect to
M13F site. PCR primers used to generate clone were
5'-CAAACAGGCAGCGCGCGACA-3' (forward) and
5'-GCTGACTTTAGCCTCTTGGTGCCC-3' (reverse).
FEATURES Location/Qualifiers
source 1..1789
/db_xref="H-InvDB:HIT000558357"
/organism="Homo sapiens"
/mol_type="mRNA"
/isolation_source="colorectal cancer cell line"
/db_xref="taxon:9606"
/clone="RKO_1800_5"
/cell_line="RKO"
/PCR_primers="fwd_seq: caaacaggcagcgcgcgaca, rev_seq:
gctgactttagcctcttggtgccc"
gene 1..1789
/gene="UMPS"
/gene_synonym="OPRT"
CDS 208..1116
/gene="UMPS"
/gene_synonym="OPRT"
/codon_start=1
/product="uridine monophosphate synthetase isoform H"
/protein_id="ACH48231.1"
/translation="MQASVLTPCVECLIQLCHWLQLSVQPIKFQCLLEGKKQRIMEAP
KELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPSICML
KTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWAD
LVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEF
VVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVGRGII
SAADRLEAAEMYRKAAWEAYLSRLGV"
BASE COUNT 447 a 387 c 448 g 507 t
ORIGIN
1 caaacaggca gcgcgcgaca atggcggtcg ctcgtgcagc tttggggcca ttggtgacgg
61 gtctgtacga cgtgcaggct ttcaagtttg gggacttcgt gctgaagagc gggctttcct
121 cccccatcta catcgatctg cggggcatcg tgtctcgacc gcgtcttctg agtcaggttg
181 cagatatttt attccaaact gcccaaaatg caggcatcag ttttgacacc gtgtgtggag
241 tgccttatac agctttgcca ttggctacag ttatctgttc aaccaatcaa attccaatgc
301 ttattagaag gaaagaaaca aaggattatg gaagcaccca aagaactcag cttcggtgca
361 cgtgcagagc tgcccaggat ccacccagtt gcatcgaagc ttctcaggct tatgcaaaag
421 aaggagacca atctgtgtct atctgctgat gtttcactgg ccagagagct gttgcagcta
481 gcagatgctt taggacctag tatctgcatg ctgaagactc atgtagatat tttgaatgat
541 tttactctgg atgtgatgaa ggagttgata actctggcaa aatgccatga gttcttgata
601 tttgaagacc ggaagtttgc agatatagga aacacagtga aaaagcagta tgaaggaggt
661 atctttaaaa tagcttcctg ggcagatcta gtaaatgctc acgtggtgcc aggctcagga
721 gttgtgaaag gcctgcaaga agtgggcctg cctttgcatc gggggtgcct ccttattgcg
781 gaaatgagct ccaccggctc cctggccact ggggactaca ctagagcagc ggttagaatg
841 gctgaggagc actctgaatt tgttgttggt tttatttctg gctcccgagt aagcatgaaa
901 ccagaatttc ttcacttgac tccaggagtt cagttggaag caggaggaga taatcttggc
961 caacagtaca atagcccaca agaagttatt ggtaaacgag gttccgatat catcattgta
1021 ggtcgtggca taatctcagc agctgatcgt ctggaagcag cagagatgta cagaaaagct
1081 gcttgggaag cgtatttgag tagacttggt gtttgagtgc ttcagataca tttttcagat
1141 acaatgtgaa gacattgaag atatgtggtc ctcctgaaag tcactggctg gaaataatcc
1201 aattattcct gcttggattc ttccacaggg cctgtgtaag aatgggttct ggagttctca
1261 tggtctttag gaaatattga gtaatttgta atcaccgcat tgatactata ataagttcat
1321 tcttaagctt gctttttttg agactggtgt ttgttagaca gccacagtcc tgtctgggtt
1381 agggtcttcc acatttgagg atccttccta tctctccatg ggactagact gctttgttat
1441 tctatttatt ttttaatttt tttcgagaca ggatctcact ctgttgccca ggatggagtg
1501 cagtggtgag atcacggctc attgcagcct cgacctccca ggtgatcctc ccacctcagc
1561 ttccagatta gctggtgcta taggcatgca ccaccacgtc catctaaatt tctttattat
1621 ttgtagagat gaggtcttgc catgttaccc aggctggtct caactcctgg gctcaagcga
1681 tcctcctgcc tcagtctctc aaagtgctgg gattacaggt gtgagccact gtgcccagcc
1741 taattgcagt aagacaaaaa ttctagggca ccaagaggct aaagtcagc
//