LOCUS       EU921891                2235 bp    mRNA    linear   HUM 27-AUG-2008
DEFINITION  Homo sapiens uridine monophosphate synthetase isoform F (UMPS)
            mRNA, complete cds.
ACCESSION   EU921891
VERSION     EU921891.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
            Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
  TITLE     Genomic analysis of UMPS expression and sequence reveals novel
            isoforms and sequence polymorphisms associated with 5-FU resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
            Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2008) Genome Sciences Centre, British Columbia
            Cancer Agency, BC Cancer Research Centre, 675 West 10th Avenue, 7th
            Floor, Vancouver, BC V5Z 1L3, Canada
COMMENT     Created by TOPO TA cloning. Direction is forward with respect to
            M13F site. PCR primers used to generate clone were
            5'-CAAACAGGCAGCGCGCGACA-3' (forward) and
            5'-GCTGACTTTAGCCTCTTGGTGCCC-3' (reverse).
FEATURES             Location/Qualifiers
     source          1..2235
                     /db_xref="H-InvDB:HIT000558355"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /isolation_source="colorectal cancer cell line"
                     /db_xref="taxon:9606"
                     /clone="RKO_2136_2"
                     /cell_line="RKO"
                     /PCR_primers="fwd_seq: caaacaggcagcgcgcgaca, rev_seq:
                     gctgactttagcctcttggtgccc"
     gene            1..2235
                     /gene="UMPS"
                     /gene_synonym="OPRT"
     CDS             396..1562
                     /gene="UMPS"
                     /gene_synonym="OPRT"
                     /codon_start=1
                     /product="uridine monophosphate synthetase isoform F"
                     /protein_id="ACH48229.1"
                     /translation="MLIRRKETKDYGTKRLVEGTINPGETCLIIEDVVTSGSSVLETV
                     EVLQKEGLKVTDAIVLLDREQGGKDKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETV
                     GRVKRFIQENVFVAANHNGSPLSIKEAPKELSFGARAELPRIHPVASKLLRLMQKKET
                     NLCLSADVSLARELLQLADALGPSICMLKTHVDILNDFTLDVMKELITLAKCHEFLIF
                     EDRKFADIGNTVKKQYEGGIFKIASWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLI
                     AEMSSTGSLATGDYTRAAVRMAEEHSEFVVGFISGSRVSMKPEFLHLTPGVQLEAGGD
                     NLGQQYNSPQEVIGKRGSDIIIVGRGIISAADRLEAAEMYRKAAWEAYLSRLGV"
BASE COUNT          563 a          475 c          574 g          623 t
ORIGIN      
        1 caaacaggca gcgcgcgaca atggcggtcg ctcgtgcagc tttggggcca ttggtgacgg
       61 gtctgtacga cgtgcaggct ttcaagtttg gggacttcgt gctgaagagc gggctttcct
      121 cccccatcta catcgatctg cggggcatcg tgtctcgacc gcgtcttctg agtcagatgg
      181 gatctctctt acgtcaccca ggctggagtg cagctctgtg atcatagctc actgcagcct
      241 ggaactcctg ggcttaagag atcttcttgc ctcgggttgc agatatttta ttccaaactg
      301 cccaaaatgc aggcatcagt tttgacaccg tgtgtggagt gccttataca gctttgccat
      361 tggctacagt tatctgttca accaatcaaa ttccaatgct tattagaagg aaagaaacaa
      421 aggattatgg aactaagcgt cttgtagaag gaactattaa tccaggagaa acctgtttaa
      481 tcattgaaga tgttgtcacc agtggatcta gtgttttgga aactgttgag gttcttcaga
      541 aggagggctt gaaggtcact gatgccatag tgctgttgga cagagagcag ggaggcaagg
      601 acaagttgca ggcgcacggg atccgcctcc actcagtgtg tacattgtcc aaaatgctgg
      661 agattctcga gcagcagaaa aaagttgatg ctgagacagt tgggagagtg aagaggttta
      721 ttcaggagaa tgtctttgtg gcagcgaatc ataatggttc tcccctttct ataaaggaag
      781 cacccaaaga actcagcttc ggtgcacgtg cagagctgcc caggatccac ccagttgcat
      841 cgaagcttct caggcttatg caaaagaagg agaccaatct gtgtctatct gctgatgttt
      901 cactggccag agagctgttg cagctagcag atgctttagg acctagtatc tgcatgctga
      961 agactcatgt agatattttg aatgatttta ctctggatgt gatgaaggag ttgataactc
     1021 tggcaaaatg ccatgagttc ttgatatttg aagaccggaa gtttgcagat ataggaaaca
     1081 cagtgaaaaa gcagtatgaa ggaggtatct ttaaaatagc ttcctgggca gatctagtaa
     1141 atgctcacgt ggtgccaggc tcaggagttg tgaaaggcct gcaagaagtg ggcctgcctt
     1201 tgcatcgggg gtgcctcctt attgcggaaa tgagctccac cggctccctg gccactgggg
     1261 actacactag agcagcggtt agaatggctg aggagcactc tgaatttgtt gttggtttta
     1321 tttctggctc ccgagtaagc atgaaaccag aatttcttca cttgactcca ggagttcagt
     1381 tggaagcagg aggagataat cttggccaac agtacaatag cccacaagaa gttattggca
     1441 aacgaggttc cgatatcatc attgtaggtc gtggcataat ctcagcagct gatcgtctgg
     1501 aagcagcaga gatgtacaga aaagctgctt gggaagcgta tttgagtaga cttggtgttt
     1561 gagtgcttca gatacatttt tcagatacaa tgtgaagaca ttgaagatat gtggtcctcc
     1621 tgaaagtcac tggctggaaa taatccaatt attcctgctt ggattcttcc acagggcctg
     1681 tgtaagaatg ggttctggag ttctcatggt ctttaggaaa tattgagtaa tttgtaatca
     1741 ccgcattgat actataataa gttcattctt aagcttgctt tttttgagac tggtgtttgt
     1801 tagacagcca cagtcctgtc tgggttaggg tcttccacat ttgaggatcc ttcctatctc
     1861 tccatgggac tagactgctt tgttattcta tttatttttt aatttttttc gagacaggat
     1921 ctcactctgt tgcccaggat ggagtgcagt ggtgagatca cggctcattg cagcctcgac
     1981 ctcccaggtg atcctcccac ctcagcttcc agattagctg gtgctatagg catgcaccac
     2041 cacgtccatc taaatttctt tattatttgt agagatgagg tcttgccatg ttacccaggc
     2101 tggtctcaac tcctgggctc aagcgatcct cctgcctcag tctctcaaag tgctgggatt
     2161 acaggtgtga gccactgtgc ccagcctaat tgcagtaaga caaaaattct agggcaccaa
     2221 gaggctaaag tcagc
//