LOCUS EU921890 2340 bp mRNA linear HUM 27-AUG-2008
DEFINITION Homo sapiens uridine monophosphate synthetase isoform E (UMPS)
mRNA, complete cds.
ACCESSION EU921890
VERSION EU921890.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2340)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Genomic analysis of UMPS expression and sequence reveals novel
isoforms and sequence polymorphisms associated with 5-FU resistance
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2340)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Direct Submission
JOURNAL Submitted (24-JUL-2008) Genome Sciences Centre, British Columbia
Cancer Agency, BC Cancer Research Centre, 675 West 10th Avenue, 7th
Floor, Vancouver, BC V5Z 1L3, Canada
COMMENT Created by TOPO TA cloning. Direction is forward with respect to
M13F site. PCR primers used to generate clone were
5'-CAAACAGGCAGCGCGCGACA-3' (forward) and
5'-GCTGACTTTAGCCTCTTGGTGCCC-3' (reverse).
FEATURES Location/Qualifiers
source 1..2340
/db_xref="H-InvDB:HIT000558354"
/organism="Homo sapiens"
/mol_type="mRNA"
/isolation_source="colorectal cancer cell line"
/db_xref="taxon:9606"
/clone="RKO-5FU_2200_1"
/cell_line="RKO/5FU"
/PCR_primers="fwd_seq: caaacaggcagcgcgcgaca, rev_seq:
gctgactttagcctcttggtgccc"
gene 1..2340
/gene="UMPS"
/gene_synonym="OPRT"
CDS 21..1049
/gene="UMPS"
/gene_synonym="OPRT"
/codon_start=1
/product="uridine monophosphate synthetase isoform E"
/protein_id="ACH48228.1"
/translation="MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIV
SRPRLLSQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKETKD
YGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAIVLLDREQGGK
DKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAANHNASPLSI
KEAPKELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPS
ICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGKCIIQE
SARIRNPA"
BASE COUNT 601 a 489 c 588 g 662 t
ORIGIN
1 caaacaggca gcgcgcgaca atggcggtcg ctcgtgcagc tttggggcca ttggtgacgg
61 gtctgtacga cgtgcaggct ttcaagtttg gggacttcgt gctgaagagc gggctttcct
121 cccccatcta catcgatctg cggggcatcg tgtctcgacc gcgtcttctg agtcaggttg
181 cagatatttt attccaaact gcccaaaatg caggcatcag ttttgacacc gtgtgtggag
241 tgccttatac agctttgcca ttggctacag ttatctgttc aaccaatcaa attccaatgc
301 ttattagaag gaaagaaaca aaggattatg gaactaagcg tcttgtagaa ggaactatta
361 atccaggaga aacctgttta atcattgaag atgttgtcac cagtggatct agtgttttgg
421 aaactgttga ggttcttcag aaggagggct tgaaggtcac tgatgccata gtgctgttgg
481 acagagagca gggaggcaag gacaagttgc aggcgcacgg gatccgcctc cactcagtgt
541 gtacattgtc caaaatgctg gagattctcg agcagcagaa aaaagttgat gctgagacag
601 ttgggagagt gaagaggttt attcaggaga atgtctttgt ggcagcgaat cataatgctt
661 ctcccctttc tataaaggaa gcacccaaag aactcagctt cggtgcacgt gcagagctgc
721 ccaggatcca cccagttgca tcgaagcttc tcaggcttat gcaaaagaag gagaccaatc
781 tgtgtctatc tgctgatgtt tcactggcca gagagctgtt gcagctagca gatgctttag
841 gacctagtat ctgcatgctg aagactcatg tagatatttt gaatgatttt actctggatg
901 tgatgaagga gttgataact ctggcaaaat gccatgagtt cttgatattt gaagaccgga
961 agtttgcaga tataggaaac acagtgaaaa agcagtatga aggtaagtgt attattcagg
1021 aatctgcaag aatcagaaat ccagcttaaa ctgaagaaga aaaaggaaat gctcatgtca
1081 ttgaaagtcc attcatagag caggctgtca agcgtggttg gatccaggag ctcaagtgat
1141 atcttcatga cttgctttct ctccatctct cagctctgct ttcttctttg ttgacttctt
1201 tctcaggagg tatctttaaa atagcttcct gggcagatct agtaaatgct cacgtggtgc
1261 caggctcagg agttgtgaaa ggcctgcaag aagtgggcct gcctttgcat cgggggtgcc
1321 tccttattgc ggaaatgagc tccaccggct ccctggccac tggggactac actagagcag
1381 cggttagaat ggctgaggag cactctgaat ttgttgttgg ttttatttct ggctcccgag
1441 taagcatgaa accagaattt cttcacttga ctccaggagt tcagttggaa gcaggaggag
1501 ataatcttgg ccaacagtac aatagcccac aagaagttat tggtaaacga ggttccgata
1561 tcatcattgt aggtcgtggc ataatctcag cagctgatcg tctggaagca gcagagatgt
1621 acagaaaagc tgcttgggaa gcgtatttga gtagacttgg tgtttgagtg cttcagatac
1681 atttttcaga tacaatgtga agacattgaa gatatgtggt cctcctgaaa gtcactggct
1741 ggaaataatc caattattcc tgcttggatt cttccacagg gcctgtgtaa gaatgggttc
1801 tggagttctc atggtcttta ggaaatattg agtaatttgt aatcaccgca ttgatactat
1861 aataagttca ttcttaagct tgcttttttg agactggtgt ttgttagaca gccacagtcc
1921 tgtctgggtt agggtcttcc acatttgagg atccttccta tctctccatg ggactagact
1981 gctttgttat tctatttatt ttttaatttt tttcgagaca ggatctcact ctgttgccca
2041 ggatggagtg cagtggtgag atcacggctc attgcagcct cgacctccca ggtgatcctc
2101 ccacctcagc ttccagatta gctggtgcta taggcatgca ccaccacgtc catctaaatt
2161 tctttattat ttgtagagat gaggtcttgc catgttaccc aggctggtct caactcctgg
2221 gctcaagcga tcctcctgcc tcagtctctc aaagtgctgg gattacaggt gtgagccact
2281 gtgcccagcc taattgcagt aagacaaaaa ttctagggca ccaagaggct aaagttcagc
//