LOCUS EU921889 2081 bp mRNA linear HUM 27-AUG-2008
DEFINITION Homo sapiens uridine monophosphate synthetase isoform D (UMPS)
mRNA, complete cds.
ACCESSION EU921889
VERSION EU921889.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2081)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Genomic analysis of UMPS expression and sequence reveals novel
isoforms and sequence polymorphisms associated with 5-FU resistance
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2081)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Direct Submission
JOURNAL Submitted (24-JUL-2008) Genome Sciences Centre, British Columbia
Cancer Agency, BC Cancer Research Centre, 675 West 10th Avenue, 7th
Floor, Vancouver, BC V5Z 1L3, Canada
COMMENT Created by TOPO TA cloning. Direction is forward with respect to
M13F site. PCR primers used to generate clone were
5'-CAAACAGGCAGCGCGCGACA-3' (forward) and
5'-GCTGACTTTAGCCTCTTGGTGCCC-3' (reverse).
FEATURES Location/Qualifiers
source 1..2081
/db_xref="H-InvDB:HIT000558353"
/organism="Homo sapiens"
/mol_type="mRNA"
/isolation_source="colorectal cancer cell line"
/db_xref="taxon:9606"
/clone="MIP5FUR_2136_2"
/cell_line="MIP/5FU"
/PCR_primers="fwd_seq: caaacaggcagcgcgcgaca, rev_seq:
gctgactttagcctcttggtgccc"
gene 1..2081
/gene="UMPS"
/gene_synonym="OPRT"
CDS 500..1408
/gene="UMPS"
/gene_synonym="OPRT"
/codon_start=1
/product="uridine monophosphate synthetase isoform D"
/protein_id="ACH48227.1"
/translation="MLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSIKEAP
KELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPSICML
KTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWAD
LVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEF
VVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVGRGII
SAADRLEAAEMYRKAAWEAYLSRLGV"
BASE COUNT 514 a 445 c 545 g 577 t
ORIGIN
1 caaacaggca gcgcgcgaca atggcggtcg ctcgtgcagc tttggggcca ttggtgacgg
61 gtctgtacga cgtgcaggct ttcaagtttg gggacttcgt gctgaagagc gggctttcct
121 cccccatcta catcgatctg cggggcatcg tgtctcgacc gcgtcttctg agtcagatgg
181 gatctctctt acgtcaccca ggctggagtg cagctctgtg atcatagctc actgcagcct
241 ggaactcctg ggcttaagag atcttcttgc ctcgggaact aagcgtcttg tagaaggaac
301 tattaatcca ggagaaacct gtttaatcat tgaagatgtt gtcaccagtg gatctagtgt
361 tttggaaact gttgaggttc ttcagaagga gggcttgaag gtcactgatg ccatagtgct
421 gttggacaga gagcagggag gcaaggacaa gttgcaggcg cacgggatcc gcctccactc
481 agtgtgtaca ttgtccaaaa tgctggagat tctcgagcag cagaaaaaag ttgatgctga
541 gacagttggg agagtgaaga ggtttattca ggagaatgtc tttgtggcag cgaatcataa
601 tggttctccc ctttctataa aggaagcacc caaagaactc agcttcggtg cacgtgcaga
661 gctgcccagg atccacccag ttgcatcgaa gcttctcagg cttatgcaaa agaaggagac
721 caatctgtgt ctatctgctg atgtttcact ggccagagag ctgttgcagc tagcagatgc
781 tttaggacct agtatctgca tgctgaagac tcatgtagat attttgaatg attttactct
841 ggatgtgatg aaggagttga taactctggc aaaatgccat gagttcttga tatttgaaga
901 ccggaagttt gcagatatag gaaacacagt gaaaaagcag tatgaaggag gtatctttaa
961 aatagcttcc tgggcagatc tagtaaatgc tcacgtggtg ccaggctcag gagttgtgaa
1021 aggcctgcaa gaagtgggcc tgcctttgca tcgggggtgc ctccttattg cggaaatgag
1081 ctccaccggc tccctggcca ctggggacta cactagggca gcggttagaa tggctgagga
1141 gcactctgaa tttgttgttg gttttatttc tggctcccga gtaagcatga aaccagaatt
1201 tcttcacttg actccaggag ttcagttgga agcaggagga gataatcttg gccaacagta
1261 caatagccca caagaagtta ttggcaaacg aggttccgat atcatcattg taggtcgtgg
1321 cataatctca gcagctgatc gtctggaagc agcagagatg tacagaaaag ctgcttggga
1381 agcgtatttg agtagacttg gtgtttgagt gcttcagata catttttcag atacaatgtg
1441 aagacattga agatatgtgg tcctcctgaa agtcactggc tggaaataat ccaattattc
1501 ctgcttggat tcttccacag ggcctgtgta agaatgggtt ctggagttct catggtcttt
1561 aggaaatatt gagtaatttg taatcaccgc attgatacta taataagttc attcttaagc
1621 ttgctttttt tgagactggt gtttgttaga cagccacagt cctgtctggg ttagggtctt
1681 ccacatttga ggatccttcc tatctctcca tgggactaga ctgctttgtt attctattta
1741 ttttttaatt tttttcgaga caggatctca ctctgttgcc caggatggag tgcagtggtg
1801 agatcacggc tcattgcagc ctcgacctcc caggtgatcc tcccacctca gcttccagat
1861 tagctggtgc tataggcatg caccaccacg tccatctaaa tttctttatt atttgtagag
1921 atgaggtctt gccatgttac ccaggctggt ctcaactcct gggctcaagc gatcctcctg
1981 cctcagtctc tcaaagtgct gggattacag gtgtgagcca ctgtgcccag cctaattgca
2041 gtaagacaaa aattctaggg caccaagagg ctaaagtcag c
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