LOCUS EU921887 1983 bp mRNA linear HUM 27-AUG-2008
DEFINITION Homo sapiens uridine monophosphate synthetase isoform B (UMPS)
mRNA, complete cds.
ACCESSION EU921887
VERSION EU921887.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1983)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Genomic analysis of UMPS expression and sequence reveals novel
isoforms and sequence polymorphisms associated with 5-FU resistance
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1983)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Direct Submission
JOURNAL Submitted (24-JUL-2008) Genome Sciences Centre, British Columbia
Cancer Agency, BC Cancer Research Centre, 675 West 10th Avenue, 7th
Floor, Vancouver, BC V5Z 1L3, Canada
COMMENT Created by TOPO TA cloning. Direction is forward with respect to
M13F site. PCR primers used to generate clone were
5'-CAAACAGGCAGCGCGCGACA-3' (forward) and
5'-GCTGACTTTAGCCTCTTGGTGCCC-3' (reverse).
FEATURES Location/Qualifiers
source 1..1983
/db_xref="H-InvDB:HIT000558351"
/organism="Homo sapiens"
/mol_type="mRNA"
/isolation_source="colorectal cancer cell line"
/db_xref="taxon:9606"
/clone="MIP5FUR_1982_6"
/cell_line="MIP/5FU"
/PCR_primers="fwd_seq: caaacaggcagcgcgcgaca, rev_seq:
gctgactttagcctcttggtgccc"
gene 1..1983
/gene="UMPS"
/gene_synonym="OPRT"
CDS 401..1309
/gene="UMPS"
/gene_synonym="OPRT"
/codon_start=1
/product="uridine monophosphate synthetase isoform B"
/protein_id="ACH48225.1"
/translation="MLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSIKEAP
KELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPSICML
KTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWAD
LVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEF
VVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVGRGII
SAADRLEAAEMYRKAAWEAYLSRLGV"
BASE COUNT 497 a 418 c 518 g 550 t
ORIGIN
1 caaacaggca gcgcgcgaca atggcggtcg ctcgtgcagc tttggggcca ttggtgacgg
61 gtctgtacga cgtgcaggct ttcaagtttg gggacttcgt gctgaagagc gggctttcct
121 cccccatcta catcgatctg cggggcatcg tgtctcgacc gcgtcttctg agtcaggaac
181 taagcgtctt gtagaaggaa ctattaatcc aggagaaacc tgtttaatca ttgaagatgt
241 tgtcaccagt ggatctagtg ttttggaaac tgttgaggtt cttcagaagg agggcttgaa
301 ggtcactgat gccatagtgc tgttggacag agagcaggga ggcaaggaca agttgcaggc
361 gcacgggatc cgcctccact cagtgtgtac attgtccaaa atgctggaga ttctcgagca
421 gcagaaaaaa gttgatgctg agacagttgg gagagtgaag aggtttattc aggagaatgt
481 ctttgtggca gcgaatcata atggttctcc cctttctata aaggaagcac ccaaagaact
541 cagcttcggt gcacgtgcag agctgcccag gatccaccca gttgcatcga agcttctcag
601 gcttatgcaa aagaaggaga ccaatctgtg tctatctgct gatgtttcac tggccagaga
661 gctgttgcag ctagcagatg ctttaggacc tagtatctgc atgctgaaga ctcatgtaga
721 tattttgaat gattttactc tggatgtgat gaaggagttg ataactctgg caaaatgcca
781 tgagttcttg atatttgaag accggaagtt tgcagatata ggaaacacag tgaaaaagca
841 gtatgaagga ggtatcttta aaatagcttc ctgggcagat ctagtaaatg ctcacgtggt
901 gccaggctca ggagttgtga aaggcctgca agaagtgggc ctgcctttgc atcgggggtg
961 cctccttatt gcggaaatga gctccaccgg ctccctggcc actggggact acactagagc
1021 agcggttaga atggctgagg agcactctga atttgttgtt ggttttattt ctggctcccg
1081 agtaagcatg aaaccagaat ttcttcactt gactccagga gttcagttgg aagcaggagg
1141 agataatctt ggccaacagt acaatagccc acaagaagtt attggcaaac gaggttccga
1201 tatcatcatt gtaggtcgtg gcataatctc agcagctgat cgtctggaag cagcagagat
1261 gtacagaaaa gctgcttggg aagcgtattt gagtagactt ggtgtttgag tgcttcagat
1321 acatttttca gatacaatgt gaagacattg aagatatgtg gtcctcctga aagtcactgg
1381 ctggaaataa tccaattatt cctgcttgga ttcttccaca gggcctgtgt aagaatgggt
1441 tctggagttc tcatggtctt taggaaatat tgagtaattt gtaatcaccg cattgatact
1501 ataataagtt cattcttaag cttgcttttt ttgagactgg tgtttgttag acagccacag
1561 tcctgtctgg gttagggtct tccacatttg aggatccttc ctatctctcc atgggactag
1621 actgctttgt tattctattt attttttaat ttttttcgag acaggatctc actctgttgc
1681 ccaggatgga gtgcagtggt gagatcacgg ctcattgcag cctcgacctc ccaggtgatc
1741 ctcccacctc agcttccaga ttagctggtg ctataggcat gcaccaccac gtccatctaa
1801 atttctttat tatttgtaga gatgaggtct tgccatgtta cccaggctgg tctcaactcc
1861 tgggctcaag cgatcctcct gcctcagtct ctcaaagtgc tgggattaca ggtgtgagcc
1921 actgtgccca gcctaattgc agtaagacaa aaattctagg gcaccaagag gctaaagtca
1981 gcc
//