LOCUS EU921886 2136 bp mRNA linear HUM 27-AUG-2008
DEFINITION Homo sapiens uridine monophosphate synthetase isoform A (UMPS)
mRNA, complete cds.
ACCESSION EU921886
VERSION EU921886.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2136)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Genomic analysis of UMPS expression and sequence reveals novel
isoforms and sequence polymorphisms associated with 5-FU resistance
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2136)
AUTHORS Griffith,M., Pugh,T.J., Tang,M.J., Asano,J.K., Ally,A., Chan,S.Y.,
Taylor,G., Morin,G.B., Tai,I.T. and Marra,M.A.
TITLE Direct Submission
JOURNAL Submitted (24-JUL-2008) Genome Sciences Centre, British Columbia
Cancer Agency, BC Cancer Research Centre, 675 West 10th Avenue, 7th
Floor, Vancouver, BC V5Z 1L3, Canada
COMMENT Created by TOPO TA cloning. Direction is forward with respect to
M13F site. PCR primers used to generate clone were
5'-CAAACAGGCAGCGCGCGACA-3' (forward) and
5'-GCTGACTTTAGCCTCTTGGTGCCC-3' (reverse).
FEATURES Location/Qualifiers
source 1..2136
/db_xref="H-InvDB:HIT000558350"
/organism="Homo sapiens"
/mol_type="mRNA"
/isolation_source="colorectal cancer cell line"
/db_xref="taxon:9606"
/clone="MIP101_2136_3"
/cell_line="MIP101"
/PCR_primers="fwd_seq: caaacaggcagcgcgcgaca, rev_seq:
gctgactttagcctcttggtgccc"
gene 1..2136
/gene="UMPS"
/gene_synonym="OPRT"
CDS 21..1463
/gene="UMPS"
/gene_synonym="OPRT"
/codon_start=1
/product="uridine monophosphate synthetase isoform A"
/protein_id="ACH48224.1"
/translation="MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIV
SRPRLLSQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKETKD
YGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAIVLLDREQGGK
DKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSI
KEAPKELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPS
ICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIA
SWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEE
HSEFVVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVG
RGIISAADRLEAAEMYRKAAWEAYLSRLGV"
BASE COUNT 545 a 447 c 548 g 596 t
ORIGIN
1 caaacaggca gcgcgcgaca atggcggtcg ctcgtgcagc tttggggcca ttggtgacgg
61 gtctgtacga cgtgcaggct ttcaagtttg gggacttcgt gctgaagagc gggctttcct
121 cccccatcta catcgatctg cggggcatcg tgtctcgacc gcgtcttctg agtcaggttg
181 cagatatttt attccaaact gcccaaaatg caggcatcag ttttgacacc gtgtgtggag
241 tgccttatac agctttgcca ttggctacag ttatctgttc aaccaatcaa attccaatgc
301 ttattagaag gaaagaaaca aaggattatg gaactaagcg tcttgtagaa ggaactatta
361 atccaggaga aacctgttta atcattgaag atgttgtcac cagtggatct agtgttttgg
421 aaactgttga ggttcttcag aaggagggct tgaaggtcac tgatgccata gtgctgttgg
481 acagagagca gggaggcaag gacaagttgc aggcgcacgg gatccgcctc cactcagtgt
541 gtacattgtc caaaatgctg gagattctcg agcagcagaa aaaagttgat gctgagacag
601 ttgggagagt gaagaggttt attcaggaga atgtctttgt ggcagcgaat cataatggtt
661 ctcccctttc tataaaggaa gcacccaaag aactcagctt cggtgcacgt gcagagctgc
721 ccaggatcca cccagttgca tcgaagcttc tcaggcttat gcaaaagaag gagaccaatc
781 tgtgtctatc tgctgatgtt tcactggcca gagagctgtt gcagctagca gatgctttag
841 gacctagtat ctgcatgctg aagactcatg tagatatttt gaatgatttt actctggatg
901 tgatgaagga gttgataact ctggcaaaat gccatgagtt cttgatattt gaagaccgga
961 agtttgcaga tataggaaac acagtgaaaa agcagtatga aggaggtatc tttaaaatag
1021 cttcctgggc agatctagta aatgctcacg tggtgccagg ctcaggagtt gtgaaaggcc
1081 tgcaagaagt gggcctgcct ttgcatcggg ggtgcctcct tattgcggaa atgagctcca
1141 ccggctccct ggccactggg gactacacta gagcagcggt tagaatggct gaggagcact
1201 ctgaatttgt tgttggtttt atttctggct cccgagtaag catgaaacca gaatttcttc
1261 acttgactcc aggagttcag ttggaagcag gaggagataa tcttggccaa cagtacaata
1321 gcccacaaga agttattggc aaacgaggtt ccgatatcat cattgtaggt cgtggcataa
1381 tctcagcagc tgatcgtctg gaagcagcag agatgtacag aaaagctgct tgggaagcgt
1441 atttgagtag acttggtgtt tgagtgcttc agatacattt ttcagataca atgtgaagac
1501 attgaagata tgtggtcctc ctgaaagtca ctggctggaa ataatccaat tattcctgct
1561 tggattcttc cacagggcct gtgtaagaat gggttctgga gttctcatgg tctttaggaa
1621 atattgagta atttgtaatc accgcattga tactataata agttcattct taagcttgct
1681 ttttttgaga ctggtgtttg ttagacagcc acagtcctgt ctgggttagg gtcttccaca
1741 tttgaggatc cttcctatct ctccatggga ctagactgct ttgttattct atttattttt
1801 taattttttt cgagacagga tctcactctg ttgcccagga tggagtgcag tggtgagatc
1861 acggctcatt gcagcctcga cctcccaggt gatcctccca cctcagcttc cagattagct
1921 ggtgctatag gcatgcacca ccacgtccat ctaaatttct ttattatttg tagagatgag
1981 gtcttgccat gttacccagg ctggtctcaa ctcctgggct caagcgatcc tcctgcctca
2041 gtctctcaaa gtgctgggat tacaggtgtg agccactgtg cccagcctaa ttgcagtaag
2101 acaaaaattc tagggcacca agaggctaaa gtcagc
//