LOCUS EU427373 824 bp mRNA linear HUM 01-MAR-2008 DEFINITION Homo sapiens clone EB81-CTL 16 truncated T cell receptor variable alpha 2 chain (TRA) mRNA, complete cds. ACCESSION EU427373 VERSION EU427373.1 KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 824) AUTHORS Straetemans,T., Broertjes,M., Drexhage,J., Coccoris,M., Lambrecht,B., van der Bruggen,P., Coulie,P., Gratama,J.W., Willemsen,R. and Debets,R. TITLE TCR gene transfer: MHC class I and II-restricted MAGE epitopes as melanoma-specific immune targets JOURNAL Unpublished REFERENCE 2 (bases 1 to 824) AUTHORS Straetemans,T., Broertjes,M., Drexhage,J., Coccoris,M., Lambrecht,B., van der Bruggen,P., Coulie,P., Gratama,J.W., Willemsen,R. and Debets,R. TITLE Direct Submission JOURNAL Submitted (24-JAN-2008) Medical Oncology, Erasmus MC - Daniel den Hoed Cancer Center, Groene Hilledijk 301, Rotterdam, ZH 3075 EA, The Netherlands FEATURES Location/Qualifiers source 1..824 /db_xref="H-InvDB:HIT000484921" /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="EB81-CTL 16" /PCR_primers="fwd_name: MC2/A2 TCR-VA2 NcoI fwd, fwd_seq: attcggccatggctttgcagagcactctgg, rev_name: TCR-CA XhoI rev, rev_seq: attcgcctcgagtcagctggaccacagccgcagcgt" /note="established from melanoma patients following MAGE vaccination" gene 1..824 /gene="TRA" /note="TCRalpha" CDS 1..435 /gene="TRA" /note="contains a frameshift in the Ja region and a premature stop codon in the constant domain; no surface expression" /codon_start=1 /product="truncated T cell receptor variable alpha 2 chain" /protein_id="ACA28838.1" /translation="MALQSTLGAVWLGLLLNSLWKVAESKDQVFQPSTVASSEGAVVE IFCNHSVSNAYNFFWYLHFPGCAPRLLVKGSKPSQQGRYNMTYERFSSSLLILQVREA DAAVYYCAVEDSQEEATYLHLEEEPALLFIRISRTLTLPCTS" BASE COUNT 209 a 206 c 193 g 216 t ORIGIN 1 atggctttgc agagcactct gggggcggtg tggctagggc ttctcctcaa ctctctctgg 61 aaggttgcag aaagcaagga ccaagtgttt cagccttcca cagtggcatc ttcagaggga 121 gctgtggtgg aaatcttctg taatcactct gtgtccaatg cttacaactt cttctggtac 181 cttcacttcc cgggatgtgc accaagactc cttgttaaag gctcaaagcc ttctcagcag 241 ggacgataca acatgaccta tgaacggttc tcttcatcgc tgctcatcct ccaggtgcgg 301 gaggcagatg ctgctgttta ctactgtgct gtggaggatt ctcaggagga agctacatac 361 ctacatttgg aagaggaacc agccttattg ttcatccgta tatccagaac cctgaccctg 421 ccgtgtacca gctgagagac tctaaatcca gtgacaagtc tgtctgccta ttcaccgatt 481 ttgattctca aacaaatgtg tcacaaagta aggattctga tgtgtatatc acagacaaaa 541 ctgtgctaga catgaggtct atggacttca agagcaacag tgctgtggcc tggagcaaca 601 aatctgactt tgcatgtgca aacgccttca acaacagcat tattccagaa gacaccttct 661 tccccagccc agaaagttcc tgtgatgtca agctggtcga gaaaagcttt gaaacagata 721 cgaacctaaa ctttcaaaac ctgtcagtga ttgggttccg aatcctcctc ctgaaagtgg 781 ccgggtttaa tctgctcatg acgctgcggc tgtggtccag ctga //