LOCUS       EU427373                 824 bp    mRNA    linear   HUM 01-MAR-2008
DEFINITION  Homo sapiens clone EB81-CTL 16 truncated T cell receptor variable
            alpha 2 chain (TRA) mRNA, complete cds.
ACCESSION   EU427373
VERSION     EU427373.1
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Straetemans,T., Broertjes,M., Drexhage,J., Coccoris,M.,
            Lambrecht,B., van der Bruggen,P., Coulie,P., Gratama,J.W.,
            Willemsen,R. and Debets,R.
  TITLE     TCR gene transfer: MHC class I and II-restricted MAGE epitopes as
            melanoma-specific immune targets
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 824)
  AUTHORS   Straetemans,T., Broertjes,M., Drexhage,J., Coccoris,M.,
            Lambrecht,B., van der Bruggen,P., Coulie,P., Gratama,J.W.,
            Willemsen,R. and Debets,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2008) Medical Oncology, Erasmus MC - Daniel den
            Hoed Cancer Center, Groene Hilledijk 301, Rotterdam, ZH 3075 EA,
            The Netherlands
FEATURES             Location/Qualifiers
     source          1..824
                     /db_xref="H-InvDB:HIT000484921"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="EB81-CTL 16"
                     /PCR_primers="fwd_name: MC2/A2 TCR-VA2 NcoI fwd, fwd_seq:
                     attcggccatggctttgcagagcactctgg, rev_name: TCR-CA XhoI rev,
                     rev_seq: attcgcctcgagtcagctggaccacagccgcagcgt"
                     /note="established from melanoma patients following MAGE
                     vaccination"
     gene            1..824
                     /gene="TRA"
                     /note="TCRalpha"
     CDS             1..435
                     /gene="TRA"
                     /note="contains a frameshift in the Ja region and a
                     premature stop codon in the constant domain; no surface
                     expression"
                     /codon_start=1
                     /product="truncated T cell receptor variable alpha 2
                     chain"
                     /protein_id="ACA28838.1"
                     /translation="MALQSTLGAVWLGLLLNSLWKVAESKDQVFQPSTVASSEGAVVE
                     IFCNHSVSNAYNFFWYLHFPGCAPRLLVKGSKPSQQGRYNMTYERFSSSLLILQVREA
                     DAAVYYCAVEDSQEEATYLHLEEEPALLFIRISRTLTLPCTS"
BASE COUNT          209 a          206 c          193 g          216 t
ORIGIN      
        1 atggctttgc agagcactct gggggcggtg tggctagggc ttctcctcaa ctctctctgg
       61 aaggttgcag aaagcaagga ccaagtgttt cagccttcca cagtggcatc ttcagaggga
      121 gctgtggtgg aaatcttctg taatcactct gtgtccaatg cttacaactt cttctggtac
      181 cttcacttcc cgggatgtgc accaagactc cttgttaaag gctcaaagcc ttctcagcag
      241 ggacgataca acatgaccta tgaacggttc tcttcatcgc tgctcatcct ccaggtgcgg
      301 gaggcagatg ctgctgttta ctactgtgct gtggaggatt ctcaggagga agctacatac
      361 ctacatttgg aagaggaacc agccttattg ttcatccgta tatccagaac cctgaccctg
      421 ccgtgtacca gctgagagac tctaaatcca gtgacaagtc tgtctgccta ttcaccgatt
      481 ttgattctca aacaaatgtg tcacaaagta aggattctga tgtgtatatc acagacaaaa
      541 ctgtgctaga catgaggtct atggacttca agagcaacag tgctgtggcc tggagcaaca
      601 aatctgactt tgcatgtgca aacgccttca acaacagcat tattccagaa gacaccttct
      661 tccccagccc agaaagttcc tgtgatgtca agctggtcga gaaaagcttt gaaacagata
      721 cgaacctaaa ctttcaaaac ctgtcagtga ttgggttccg aatcctcctc ctgaaagtgg
      781 ccgggtttaa tctgctcatg acgctgcggc tgtggtccag ctga
//