LOCUS HUMCALP 1156 bp mRNA linear HUM 15-JUN-2010
DEFINITION Homo sapiens mRNA for calpastatin, partial cds.
ACCESSION D50827
VERSION D50827.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1156)
AUTHORS Mimori,T.
TITLE Direct Submission
JOURNAL Submitted (30-MAY-1995) to the DDBJ/EMBL/GenBank databases.
Contact:Tsuneyo Mimori
Keio University School of Medicine, Department of Internal
Medicine; 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan
REFERENCE 2
AUTHORS Asada,K., Ishino,Y., Shimada,M., Shimojo,T., Endo,M., Kimizuka,F.,
Kato,I., Maki,M., Hatanaka,M. and Murachi,T.
TITLE cDNA cloning of human calpastatin: sequence homology among human,
pig, and rabbit calpastatin
JOURNAL J. Enzym. Inhib. 3, 49-56 (1989)
REFERENCE 3
AUTHORS Mimori,T., Suganuma,K., Tanami,Y., Nojima,T., Matsumura,M.,
Fujii,T., Yoshizawa,T., Suzuki,K. and Akizuki,M.
TITLE Autoantibodies to calpastatin (an endogenous inhibitor for
calcium-dependent neutral protease, calpain) in systemic rheumatic
diseases
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 92, 7267-7271 (1995)
COMMENT
FEATURES Location/Qualifiers
source 1..1156
/cell_line="HeLa cell"
/clone="RA-6"
/clone_lib="lambda gt11"
/db_xref="H-InvDB:HIT000101639"
/db_xref="taxon:9606"
/mol_type="mRNA"
/organism="Homo sapiens"
CDS <1..537
/citation=[2]
/codon_start=1
/experiment="experimental evidence, no additional details
recorded"
/function="inhibitor of calapin (calcium-dependent
neutral protease)"
/product="calpastatin"
/protein_id="BAA09438.1"
/translation="EDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKL
SDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDDNGQDKPVK
PPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASSSKAPKNGG
KAKDSAKTTEETSKPKDD"
misc_difference 185
/citation=[2]
/note="conflict: This nucleotide replacement causes an
amino acid replacement of the translated product (E at AA
position 61 of RA-6 to G at AA position 557 of citation
#2)."
/replace="g"
3'UTR 538..1156
/inference="non-experimental evidence, no additional
details recorded"
misc_difference 735..1156
/citation=[2]
/note="deletion, Additional sequence of 3'-untranslated
region (bp735-1156) in human calpastatin mRNA that was
not described in citation #2."
/replace=""
regulatory 1115..1120
/regulatory_class="polyA_signal_sequence"
BASE COUNT 391 a 223 c 228 g 314 t
ORIGIN
1 gaagatgcta aacttgctgc tgccatctct gaagtggttt cccaaacccc agcttcaacg
61 acccaagctg gagccccacc ccgtgatacc tcgcagagtg acaaagacct cgatgatgcc
121 ttggataaac tctctgacag tctaggacaa aggcagcctg acccagatga gaacaaacca
181 atggaagata aagtaaagga aaaagctaaa gctgaacata gagacaagct tggagaaaga
241 gatgacacta tcccacctga atacagacat ctcctggatg ataatggaca ggacaaacca
301 gtgaagccac ctacaaagaa atcagaggat tcaaagaaac ctgcagatga ccaagacccc
361 attgatgctc tctcaggaga tctggacagc tgtccctcca ctacagaaac ctcacagaac
421 acagcaaagg ataagtgcaa gaaggctgct tccagctcca aagcacctaa gaatggaggt
481 aaagcgaagg attcagcaaa gacaacagag gaaacttcca agccaaaaga tgactaaaga
541 aatacaagtt aaggtatctg gtatctgcat gtaaaatctt cagctggtgg atggtgactt
601 ttgaagaaca aaaggctttg gcaacagaaa acaattgttc tgggtgattt ctagaatggt
661 ttttgttgag tctctgaaca tcctaaatat tggtttgtta ttcttttcca gaaagaaaat
721 gaatttgact ggttcacctg tgtactgagt attgataaac tttgaatttt tttaattgcc
781 ttcaattggg agagaaagct ttatatttgt aagaaatata tttgataaag tttcttaaag
841 caacaccaaa aaaacaaaag aaaagctaag tgaatttttt gcacattcta cacacagtgc
901 ctgtaaatct catttgtatt ttcagtttgc ccttaatttt ttttgttagt gtttagaaaa
961 caatgtttta aacattcttc agtgttctga tttcttatta ccccctttcc tcttgggctt
1021 ttgaactgta tttgatgttg ctttgggata atgtttataa gtgaaacata agatattgta
1081 cattgggcac atatctcctc ttgggctgct aaataataaa ttaataacag gtaacctgga
1141 caaaccagga agcatt
//