LOCUS D49818 1980 bp mRNA linear HUM 10-FEB-1999
DEFINITION Homo sapiens mRNA for 6-phosphofructo-2-kinase/fructose-2,6-
bisphosphatase, complete cds.
ACCESSION D49818
VERSION D49818.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1980)
AUTHORS Sakakibara,R.
TITLE Direct Submission
JOURNAL Submitted (22-MAR-1995) to the DDBJ/EMBL/GenBank databases.
Contact:Ryozo Sakakibara
Nagasaki University, School of Pharmaceutical Sciences, Department
of Biochemistry; 1-14 Bunkyo-machi, Nagasaki, Nagasaki 852, Japan
REFERENCE 2
AUTHORS Sakakibara,R.
JOURNAL Unpublished (1996)
REFERENCE 3
AUTHORS Sakai,A., Kato,M., Fukasawa,M., Ishiguro,M., Furuya,E. and
Sakakibara,R.
TITLE Cloning of cDNA encoding for a novel isozyme of fructose
6-phosphate,2-kinase/fructose 2,6-bisphosphatase from human
placenta
JOURNAL J. Biochem. 119, 506-511 (1996)
COMMENT Sequence updated (20-Jan-1997) by: Ryozo Sakakibara
Sequence updated (19-Mar-1997) by: Ryozo Sakakibara
FEATURES Location/Qualifiers
source 1..1980
/cell_type="chorionic villi"
/clone="2K-1"
/clone_lib="lambda gt10"
/db_xref="H-InvDB:HIT000101531"
/db_xref="taxon:9606"
/dev_stage="first trimester"
/mol_type="mRNA"
/organism="Homo sapiens"
/tissue_type="placenta"
CDS 27..1436
/codon_start=1
/function="bifunctional enzyme"
/note="EC 2.7.1.105 / EC 3.1.3.46"
/product="6-phosphofructo-2-kinase/fructose-2,6-
bisphosphatase"
/protein_id="BAA18921.1"
/translation="MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVM
VGLPARGKTYISKKLTRYLNWIGVPTREFNVGQYRRDVVKTYKSFEFFLPDNEEGLKI
RKQCALAALRDVRRFLSEEGGHVAVFDATNTTRERRATIFNFGEQNGYKTFFVESICV
DPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKI
MDVGQSYVVNRVADHIQSRIVYYLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSP
RGREFAKSLAQFISDQNIKDLKVWTSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEE
MTYEEIQDNYPLEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQ
AVMRCLLAYFLDKAAEQLPYLKCPLHTVLKLTPVAYGCKVESIFLNVAAVNTHRDRPQ
NVDISRPPEEALVTVPAHQ"
BASE COUNT 443 a 561 c 561 g 415 t
ORIGIN
1 gcagtccgac tcatcccggc cccgggatgg cgtccccacg ggaattgaca cagaaccccc
61 tgaagaagat ctggatgcca tacagcaatg ggcggcccgc tctgcacgct tgccagcgcg
121 gtgtgtgcat gaccaactgc ccaactctca ttgtcatggt gggcctgccc gccaggggca
181 agacctacat ctccaagaag ctgactcgat acctgaactg gattggtgtg cccactcggg
241 agttcaatgt tggccagtac cgccgggacg tggtcaagac ctacaaatct tttgaatttt
301 ttctccccga caatgaagag ggcctgaaaa tcaggaagca gtgtgccctg gcagccctcc
361 gtgacgtccg gcggttcctt agtgaggagg ggggacatgt ggcggttttt gatgccacaa
421 acaccacccg agaacggaga gcgaccatct ttaattttgg agaacagaat ggctacaaga
481 ccttttttgt cgagtccatc tgtgtggatc ctgaggtcat agctgccaac atcgtgcaag
541 tgaaactggg cagccctgac tatgtcaacc gcgacagtga tgaggctacg gaggacttca
601 tgaggcgcat tgagtgctat gagaactcct acgagtcgct agatgaggac ctggataggg
661 acctgtccta tatcaagatc atggatgtgg gccagagcta cgtggtgaac cgtgtggctg
721 accacatcca gagccgcatc gtatattacc tcatgaacat ccacgtgacc ccccgctcca
781 tctacctctg ccggcacggg gagagcgagc tcaacctcaa gggccggatt ggcggggacc
841 caggactgtc ccctcggggc agggagtttg ccaagagtct agcccagttc atcagtgacc
901 aaaatatcaa ggatctgaag gtctggacaa gccagatgaa gaggacaatc cagacggctg
961 aggcactggg tgtgccctat gaacagtgga aggtcctcaa cgagatcgat gcgggcgtct
1021 gtgaggaaat gacctacgag gaaattcagg ataattatcc actggagttc gccctgcggg
1081 accaggacaa gtaccggtac cggtacccta aaggggagtc ctacgaggac ctggtccaga
1141 gactggagcc tgtcatcatg gagctggaga ggcaagagaa tgtgctggtc atctgccacc
1201 aggctgtgat gcgctgcctg ctggcctact tcctcgacaa ggcagcagaa cagctgccct
1261 acctcaagtg tccgctgcac acagtcctga agctgactcc tgtggcatat ggttgtaaag
1321 tggagtccat attcctgaac gtggctgctg tgaacacgca ccgggacagg cctcagaacg
1381 tggacatctc aagacctcca gaggaagccc ttgtcacggt gcctgctcac cagtgaccat
1441 gttcatccac tgtgaccact aggcaggcac tgctctctgc agagggggtc attccaggcc
1501 ctccagtgtg tgtgatagtc accatgccat gcagggatat tcttaaagcc acacatggct
1561 ggcggaaccc agagccccca ccccagccac ctggctcttt gttgacagtc ggcgacaagg
1621 ttgtgcgtgg ctcctgacct gctgctaaga gtcacttgac cagactgcat ctgcatgggc
1681 tgcgcggagg ttgcccagcc ccagtttctt ccggcgcagc tcttaggtgt tcactctcgc
1741 cagctcagtt ggctttgtga agtgtgaaac cctacaatgt gaaaggaaag tgcttgctgt
1801 gatgttccta ctgtggccca gctgcccagc atggacctgg tgactctcca cagggcctct
1861 accatcctct ctgtggccac ttcctgagcc agaggccagg tcttcatggg gccctgagct
1921 tctgctgcct ctggtgagag ggagagccct tcccatcctt acccaccagg aactagagcc
//