LOCUS HUMITS 4494 bp mRNA linear HUM 08-FEB-2003
DEFINITION Homo sapiens mRNA for isoleucyl-tRNA synthetase, complete cds.
ACCESSION D28473
VERSION D28473.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 4494)
AUTHORS Shiba,K.
TITLE Direct Submission
JOURNAL Submitted (08-FEB-1994) to the DDBJ/EMBL/GenBank databases.
Contact:Kiyotaka Shiba
Cancer Institute, Department of Cell Biology; 1-37-1,
Kami-Ikebukuro, Toshima-ku, Tokyo 170, Japan
REFERENCE 2
AUTHORS Shiba,K., Suzuki,N., Shigesada,K., Namba,Y., Schimmel,P. and
Noda,T.
TITLE Human cytoplasmic isoleucyl-tRNA synthetase: selective divergence
of the anticodon-binding domain and acquisition of a new
structural unit
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 91, 7435-7439 (1994)
COMMENT
FEATURES Location/Qualifiers
source 1..4494
/cell_line="KUT-2"
/cell_type="T-lymphocyte"
/db_xref="H-InvDB:HIT000101297"
/db_xref="taxon:9606"
/mol_type="mRNA"
/organism="Homo sapiens"
misc_feature 1..232
/note="RACE-PCR product from human brain mRNA"
CDS 230..4030
/codon_start=1
/product="isoleucyl-tRNA synthetase"
/protein_id="BAA05835.1"
/translation="MSNKMLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPK
FTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDK
TLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFM
ESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEE
DETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESD
YEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGA
EDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLL
VATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFG
NWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVD
HLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGID
QTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGA
DALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEF
LYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVR
MNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQ
DKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVV
IHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFK
AVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQ
ALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVI
ESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVN
LNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQT
DLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPL
GQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPT
TADF"
regulatory 4476..4481
/regulatory_class="polyA_signal_sequence"
polyA_site 4494
BASE COUNT 1297 a 942 c 1031 g 1224 t
ORIGIN
1 gccggatgag ttgcttttag gcttgctggc ccgcggggct gtccaggcac gcgaggcccc
61 tcaggtacgc cctctcttcc ctgcaggatc cggccctcaa agacgagggt cacgcacgcg
121 ttacaacccc gaaacagtag cacaagattt aatttttaaa agagcgtgtt tcttcggggc
181 ttgccgttcg ttcgtttcca gcctcaggaa tttatggtcg cctttttgaa tgagcaacaa
241 aatgcttcaa caagttccag aaaacataaa ttttcctgct gaagaagaga aaatcttgga
301 gttttggact gaatttaatt gttttcagga atgcttaaag caatcaaaac ataaaccaaa
361 atttaccttc tatgatggtc ctccttttgc aactggactg cctcactatg gacatatact
421 tgcgggtaca attaaagata tagttacaag atatgctcac cagagtgggt ttcatgttga
481 cagaagattt ggatgggatt gccatggctt acctgtggaa tatgaaattg ataagacact
541 gggaatcaga ggaccagagg atgtggccaa aatggggatt acagagtata acaatcagtg
601 ccgagcaatt gtgatgagat attctgctga gtggaagtct actgttagca gacttggccg
661 atggattgac tttgacaatg actataaaac tctgtatcca caattcatgg aatcagtctg
721 gtgggtcttc aaacaactct atgataaagg ccttgtttat agaggtgtga aagtcatgcc
781 cttctctacg gcatgtaaca ctccactttc caacttcgag tcacaccaga attataagga
841 tgttcaagat ccttcagtat ttgtaacttt ccctttggaa gaagatgaaa ctgtatcttt
901 agttgcttgg acaaccactc cctggactct acctagtaac cttgctgtgt gtgttaatcc
961 agaaatgcaa tatgtgaaaa ttaaagatgt tgccagagga cgattactca ttttaatgga
1021 agccagattg tcagccctct ataaattgga gagtgactat gagatccttg aaagatttcc
1081 tggtgcctat cttaaaggca agaagtacag gcccctgttt gactatttcc tgaagtgtaa
1141 agagaatggc gctttcactg tgcttgttga caactatgtg aaggaagaag aaggcacagg
1201 ggttgtccac caagctcctt acttcggtgc tgaggactat cgggtctgta tggactttaa
1261 cattattcgg aaagactcac tccctgtttg ccctgtggat gcttcaggct gcttcacaac
1321 ggaggtgaca gatttcgcag gacagtatgt gaaggatgct gacaaaagta tcatcaggac
1381 tttgaaggaa caaggccgac ttctggttgc caccaccttc actcacagct accctttttg
1441 ctggagatca gacactcctc taatttacaa agcagtgccc agctggtttg tgcgagtgga
1501 gaacatggtg gaccagctcc taaggaacaa tgacctgtgc tactgggtcc cagagttggt
1561 acgagaaaaa cgatttggaa attggctgaa agatgcacgt gactggacaa tttccagaaa
1621 cagatactgg ggcaccccca tcccactgtg ggtcagcgat gactttgagg aggtggtatg
1681 cattgggtca gtggcggaac ttgaagaact gtcaggagca aagatctcag atctccacag
1741 agagagtgtt gaccacctga ccattccttc acgctgtggg aagggatcct tgcaccgcat
1801 ctctgaagtg tttgactgtt ggtttgagag tggcagcatg ccctatgctc aggttcatta
1861 cccgtttgaa aacaagaggg agtttgagga tgcttttcct gcagatttca ttgccgaggg
1921 catcgaccaa accagaggat ggttttatac cctgctggtg ctggccacgg ccctctttgg
1981 acaaccgcct ttcaagaacg taattgtgaa tgggcttgtc ctggcaagtg atggccaaaa
2041 aatgagcaaa cggaaaaaga attatccaga tccagtttcc atcatccaga agtatggtgc
2101 tgatgccctc agattatatc tgattaactc ccctgtggtg agagcagaaa acctccgctt
2161 taaagaagag ggtgtgcggg acgtccttaa ggatgtactg ctcccatggt acaatgccta
2221 tcgcttctta atccagaacg ttctgaggct ccagaaggag gaagaaatag aatttctcta
2281 caatgagaac acggttagag aaagccccaa cattacagac cggtggatcc tgtccttcat
2341 gcagtctctc attggcttct ttgagactga aatggcagct tataggcttt atactgtggt
2401 gcctcgcctg gtcaagtttg tagatattct gaccaattgg tatgttagaa tgaaccgcag
2461 aagattaaag ggtgaaaatg ggatggagga ttgtgtcatg gccctagaaa ccttgtttag
2521 tgttctgctt tctctttgca gacttatggc tccctacaca ccttttctca ctgaattgat
2581 gtaccagaat ctaaaggtgc tgattgaccc tgtttctgtt caggacaagg acacactcag
2641 cattcactac ctcatgctgc cccgtgttcg agaagaattg attgacaaga aaacagagag
2701 tgcagtatct cagatgcagt ctgtgattga acttggaaga gtgatcagag accgaaaaac
2761 tattcccata aagtatcctt tgaaagaaat tgtggttatc catcaagatc cagaagctct
2821 taaagatatc aagtctttgg agaagtatat cattgaggaa ctcaatgttc gaaaagttac
2881 actgtctaca gataaaaaca agtatggcat tcggctaagg gcagaaccag atcacatggt
2941 cctggggaag cgtctgaagg gagcctttaa ggcagtgatg acgtccatca agcagttgag
3001 cagtgaggag ctggagcagt tccagaagac tgggaccatt gttgtggaag gccatgaatt
3061 gcacgatgaa gacatccgcc tcatgtacac ctttgatcag gccacaggtg ggactgcgca
3121 atttgaagca cactcagatg ctcaggcttt ggtcctctta gatgtcactc ctgaccagtc
3181 aatggtagat gaaggaatgg ctcgggaagt catcaatcgc atacagaaac ttcgcaaaaa
3241 gtgcaatctg gttccaactg atgaaatcac agtgtactat aaagcaaagt ctgaaggaac
3301 atatctgaat agtgttattg aaagccacac agagttcata tttaccacca taaaggctcc
3361 cttgaaacca tatccagttt ctccatcgga taaagtcctt attcaagaaa aaacacagtt
3421 gaagggatct gaactggaaa ttacactcac cagaggatct tcccttcctg gtcctgcttg
3481 tgcatatgtc aatcttaaca tttgtgcaaa tggcagtgaa caaggtggag tattgctcct
3541 ggaaaatcca aaaggtgaca ataggttgga ccttttaaag ctgaagagtg ttgtcactag
3601 catttttggt gtgaaaaata cagagctggc tgtcttccat gatgaaacag aaatacaaaa
3661 ccaaactgac ttactgagtc ttagtggaaa aacactttgt gtgactgcag gatcggctcc
3721 ctctctgatc aacagttcta gtactcttct ttgtcagtat atcaacctac agctcctgaa
3781 tgcaaagcca caagagtgtt taatggggac agtgggcact ctcctgcttg aaaacccact
3841 tgggcagaat ggactcaccc accaaggtct tctgtatgaa gcagccaagg tgtttggcct
3901 tcggagcagg aagctaaagc tgtttctgaa tgagacccaa acgcaggaaa ttacagaaga
3961 catccccgtg aagactttga atatgaagac tgtgtatgtt tctgtgttac caacaacagc
4021 agacttctag catgtactta tcaatgttgt tcggtcagcc cttccctaat tacacctatc
4081 ccctacacat acatgcacat agacacacac atgaacacac tgaagatatt tccttcaggt
4141 gtgtgtaaaa tatgctgctt ggattgaaat tcaaatggga ttgattagtc aagtaacttg
4201 agacctcaca gtaatcttca cacttaacct tagacaccta tgcagtcatg ttgggagcag
4261 gttacaatgt tacttcagcc cacagtttat ttctatactt gagttcttaa gtacagaaga
4321 tagaagtgat ttaaatggca tagtatatat atcattttct ggccttttaa aatttatttg
4381 agacctcttg atgaaatgga catattatat atttctgcca cctggatttt cctggataat
4441 ttgatggaat attttaagtt tcagtaaatc agaacaataa acaaactcag atat
//