LOCUS HUMPLCALF1 1915 bp mRNA linear HUM 13-FEB-2003
DEFINITION Homo sapiens PLC alpha mRNA for phospholipase C-alpha, complete
cds.
ACCESSION D16234
VERSION D16234.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1915)
AUTHORS Hirano,N.
TITLE Direct Submission
JOURNAL Submitted (12-MAY-1993) to the DDBJ/EMBL/GenBank databases.
Contact:Naoto Hirano
University of Tokyo, Third Department of Internal Medicine; 7-3-1
Hongo, Bunkyo-ku, Tokyo 113, Japan
REFERENCE 2
AUTHORS Hirano,N., Shibasaki,F., Katoh,H., Sakai,R., Tanaka,T.,
Nishida,J., Yazaki,Y., Takenawa,T. and Hirai,H.
TITLE Molecular cloning and characterization of a cDNA for bovine
phospholipase C-alpha: proposal of redesignation of phospholipase
C-alpha
JOURNAL Biochem. Biophys. Res. Commun. 204, 375-382 (1994)
COMMENT
FEATURES Location/Qualifiers
source 1..1915
/clone="lambda 17"
/clone_lib="lambda ZAPII library of H.Toyoshima"
/db_xref="H-InvDB:HIT000100576"
/db_xref="taxon:9606"
/mol_type="mRNA"
/organism="Homo sapiens"
CDS 68..1585
/codon_start=1
/gene="PLC alpha"
/note="redox regulating protein family"
/note="secretory protein"
/product="phospholipase C-alpha"
/protein_id="BAA03759.1"
/translation="MRLRRLALFPGVALLLAAARLAAASDVLELTDDNFESRISDTGS
AGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPT
LKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFF
DDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEYK
TVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKDAKGS
NYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGE
KFVMQEEFSRDGKALERFLQGYFGGNLKRYLKSDPIPESNDGPVKVVVAENFDEIVNN
ENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGF
PTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL"
sig_peptide 68..139
misc_feature 233..250
/note="active site"
misc_feature 1280..1297
/note="active site"
regulatory 1890..1895
/regulatory_class="polyA_signal_sequence"
BASE COUNT 554 a 403 c 475 g 483 t
ORIGIN
1 ggcgccgacc tccgcagtcc cagccgagcc gcgacccttc cggccgtccc caccccacct
61 cgccgccatg cgcctccgcc gcctagcgct gttcccgggt gtggcgctgc ttcttgccgc
121 ggcccgcctc gccgctgcct ccgacgtgct agaactcacg gacgacaact tcgagagtcg
181 catctccgac acgggctctg cgggcctcat gctcgtcgag ttcttcgctc cctggtgtgg
241 acactgcaag agacttgcac ctgagtatga agctgcagct accagattaa aaggaatagt
301 cccattagca aaggttgatt gcactgccaa cactaacacc tgtaataaat atggagtcag
361 tggatatcca accctgaaga tatttagaga tggtgaagaa gcaggtgctt atgatggacc
421 taggactgct gatggaattg tcagccactt gaagaagcag gcaggaccag cttcagtgcc
481 tctcaggact gaggaagaat ttaagaaatt cattagtgat aaagatgcct ctatagtagg
541 ttttttcgat gattcattca gtgaggctca ctccgagttc ctaaaagcag ccagcaactt
601 gagggataac taccgatttg cacatacgaa tgttgagtct ctggtgaacg agtatgatga
661 taatggagag ggtatcatct tatttcgtcc ttcacatctc actaacaagt ttgagtacaa
721 gactgtggca tatacagagc aaaaaatgac cagtggcaaa attaaaaagt ttatccagga
781 aaacattttt ggtatctgcc ctcacatgac agaggacaat aaagatttga tacagggcaa
841 ggacttactt attgcttact atgatgtgga ctatgaaaag gacgctaaag gttccaacta
901 ctggagaaac agggtaatga tggtggcaaa gaaattcctg gatgctgggc acaaactcaa
961 ctttgctgta gctagccgca aaacctttag ccatgaactt tctgattttg gcttggagag
1021 cactgctgga gagattcctg ttgttgctat caggactgct aaaggagaga agtttgtcat
1081 gcaggaggag ttctcgcgtg atgggaaggc tctggagagg ttcctgcagg gttactttgg
1141 tggcaatctg aagagatacc tgaagtctga ccctatccca gagagcaatg atgggcctgt
1201 gaaggtagtg gtagcagaga attttgatga aatagtgaat aatgaaaata aagatgtgct
1261 gattgaattt tatgcccctt ggtgtggtca ttgtaagaac ctggagccca agtataaaga
1321 acttggcgag aagctcagca aagacccaaa tatcgtcata gccaagatgg atgccacagc
1381 caatgatgtg ccttctccat atgaagtcag aggttttcct accatatact tctctccagc
1441 caacaagaag ctaaatccaa agaaatatga aggtggccgt gaattaagtg attttattag
1501 ctatctacaa agagaagcta caaacccccc tgtaattcaa gaagaaaaac ccaagaagaa
1561 gaagaaggca caggaggatc tctaaagcag tagccaaaca ccactttgta aaaggactct
1621 tccatcagag atggaaaacc attggggagg gactaggacc catatgggaa ttattacctc
1681 tcagggccga gaggacagaa tggaaataat ctgaatcctg ttaaattttc tctaaactgt
1741 ttcttagctg cactgtttat ggaaatacca ggaaccagtt tatgtttgtg gttttgggaa
1801 aaattatttg tgttggggga aatgttgtgg gggtggggtt gagttggggg atattttcta
1861 attttttttg tacatttgga acagtgacca ataaatgaga cccctttaaa ctgtc
//