LOCUS HUMCAST 2493 bp mRNA linear HUM 03-JUN-2021
DEFINITION Homo sapiens CAST mRNA for calpastatin, complete cds.
ACCESSION D16217
VERSION D16217.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2493)
AUTHORS Maki,M.
TITLE Direct Submission
JOURNAL Submitted (08-MAY-1993) to the DDBJ/EMBL/GenBank databases.
Contact:Masatoshi Maki
Kyoto University Institute for Virus Res.; 53
Shogoin-Kawahara-cho, Sakyo-ku, Kyoto 606-01, Japan
REFERENCE 2
AUTHORS Asada,K., Ishino,Y., Shimada,M., Shimojo,T., Endo,M., Kimizuka,F.,
Kato,I., Maki,M., Hatanaka,M. and Murachi,T.
TITLE cDNA cloning of human calpastatin: sequence homology among human,
pig, and rabbit calpastatin
JOURNAL J. Enzym. Inhib. 3, 49-56 (1989)
REFERENCE 3
AUTHORS Lee,W., Ma,H., Takano,E., Yang,H., Hatanaka,M. and Maki,M.
TITLE Molecular diveersity in Amino-terminal domains of Human
calpastatin by exon skipping
JOURNAL J. Biol. Chem. 267, 8437-8442 (1992)
COMMENT The sequence from bp1 to bp123 corresponds to exon 1
(unpublished), the sequence from bp163 to bp1113 corresponds to
exon 2-14 (citation=[3]) and the sequence from bp1114 to bp2493
corresponds to cDNA from liver (citation=[2]).
FEATURES Location/Qualifiers
source 1..2493
/cell_type="leukocyte"
/chromosome="5"
/db_xref="H-InvDB:HIT000100573"
/db_xref="taxon:9606"
/map="5q14-q22"
/mol_type="mRNA"
/organism="Homo sapiens"
/tissue_type="liver"
CDS 163..2289
/codon_start=1
/function="calpain inhibitor"
/gene="CAST"
/note="animal proteinase inhibitor"
/note="tissue specific splicing"
/product="calpastatin"
/protein_id="BAA03747.1"
/translation="MNPTETKAIPVSQQMEGPHLPNKKKHKKQAVKTEPEKKSQSTKL
SVVHEKKSQEGKPKEHTEPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTK
PQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDL
IDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGP
PADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQE
KKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDET
IPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEKT
EESKAAAPAPVSEAVSRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPED
GKPVMDKVKEKAKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSE
DFLLDALSEDFSGPQNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKD
LDDALDKLSDSLGQRQPDPDENKPMGDKVKEKAKAEHRDKLGERDDTIPPEYRHLLDD
NGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTAKDKCKKAASS
SKAPKNGGKAKDSAKTTEETSKPKDD"
misc_difference 831
/citation=[3]
/inference="similar to sequence:INSD:M86258.1"
/note="conflict: Base substitution has occurred at this
position in cDNA from WI38 (ACC#: M86258)"
/replace="g"
BASE COUNT 882 a 545 c 571 g 495 t
ORIGIN
1 acattggtaa ggtgttttcc cccattgaaa ctaatatttt ctattttcaa gaaaaaagca
61 gcaagcctcg gcagcagtca atcctccaga acctatgctg gtggaacagc ctcggccacc
121 aaggtgtcag cttcctctgg tgcaaccagc aagtcttcca gtatgaatcc cacagaaacc
181 aaggccattc cagtcagcca acagatggaa ggaccacatc ttcctaacaa gaaaaaacac
241 aaaaaacagg ctgtaaaaac agaacctgag aagaagtcac agtcaaccaa gctgtctgtg
301 gttcatgaga aaaaatccca agaaggaaag ccaaaagaac acacagagcc aaaaagccta
361 cccaagcagg catcagatac aggaagtaac gatgctcaca ataaaaaagc agtttccaga
421 tcagctgaac agcagccatc agagaaatca acagaaccaa agactaaacc acaagacatg
481 atttctgctg gtggagagag tgttgctggt atcactgcaa tatctggcaa gccgggtgac
541 aagaaaaaag aaaagaaatc attaacccca gctgtgccag ttgaatctaa accggataaa
601 ccatcgggaa agtcaggcat ggatgctgct ttggatgact taatagatac tttaggagga
661 cctgaagaaa ctgaagaaga aaatacaacg tatactggac cagaagtttc agatccaatg
721 agttccacct acatagagga attgggtaaa agagaagtca caattcctcc aaaatatagg
781 gaactattgg ctaaaaagga agggatcaca gggcctcctg cagactcttc aaaacccata
841 gggccagatg atgctataga cgccttgtca tctgacttca cctgtgggtc gcctacagct
901 gctggaaaga aaactgaaaa agaggaatct acagaagttt taaaagctca gtcagcaggg
961 acagtcagaa gtgctgctcc accccaagag aagaaaagaa aggtggagaa ggatacaatg
1021 agtgatcaag cactcgaggc tctgtcggct tcactgggca cccggcaagc agaacctgag
1081 ctcgacctcc gctcaattaa ggaagtcgat gaggcaaaag ctaaagaaga aaaactagag
1141 aagtgtggtg aggatgatga aacaatccca tctgagtaca gattaaaacc agccacggat
1201 aaagatggaa aaccactatt gccagagcct gaagaaaaac ccaagcctcg gagtgaatca
1261 gaactcattg atgaactttc agaagatttt gaccggtctg aatgtaaaga gaaaccatct
1321 aagccaactg aaaagacaga agaatctaag gccgctgctc cagctcctgt gtcggaggct
1381 gtgtctcgga cctccatgtg tagtatacag tcagcacccc ctgagccggc taccttgaag
1441 ggcacagtgc cagatgatgc tgtagaagcc ttggctgata gcctggggaa aaaggaagca
1501 gatccagaag atggaaaacc tgtgatggat aaagtcaagg agaaggccaa agaagaagac
1561 cgtgaaaagc ttggtgaaaa agaagaaaca attcctcctg attatagatt agaagaggtc
1621 aaggataaag atggaaagcc actcctgcca aaagagtcta aggaacagct tccacccatg
1681 agtgaagact tccttctgga tgctttgtct gaggacttct ctggtccaca aaatgcttca
1741 tctcttaaat ttgaagatgc taaacttgct gctgccatct ctgaagtggt ttcccaaacc
1801 ccagcttcaa cgacccaagc tggagcccca ccccgtgata cctcgcagag tgacaaagac
1861 ctcgatgatg ccttggataa actctctgac agtctaggac aaaggcagcc tgacccagat
1921 gagaacaaac caatgggaga taaagtaaag gaaaaagcta aagctgaaca tagagacaag
1981 cttggagaaa gagatgacac tatcccacct gaatacagac atctcctgga tgataatgga
2041 caggacaaac cagtgaagcc acctacaaag aaatcagagg attcaaagaa acctgcagat
2101 gaccaagacc ccattgatgc tctctcagga gatctggaca gctgtccctc cactacagaa
2161 acctcacaga acacagcaaa ggataagtgc aagaaggctg cttccagctc caaagcacct
2221 aagaatggag gtaaagcgaa ggattcagca aagacaacag aggaaacttc caagccaaaa
2281 gatgactaaa gaaatacaag ttaaggtatc tggtatctgc atgtaaaatc ttcagctggt
2341 ggatggtgac ttttgaagaa caaaaggctt tggcaacaga aaacaattgt tctgggtgat
2401 ttctagaatg gtttttgttg agtctctgaa catcctaaat attggtttgt tattctttcc
2461 agaaagaaaa tgaatttgac tggttcggaa ttc
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