LOCUS HUMADH21C 2485 bp mRNA linear HUM 16-APR-1998
DEFINITION Homo sapiens mRNA for alcohol dehydrogenase beta 1, complete cds.
ACCESSION D00137
VERSION D00137.1
KEYWORDS .
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1
AUTHORS Yokoyama,S., Yokoyama,R. and Rotwein,P.
TITLE Molecular characterization of cDNA clones encoding the human
alcohol dehydrogenase beta 1 and the evolutionary relationship to
the other class I subunits alpha and gamma
JOURNAL Jpn. J. Genet. 62, 241-256 (1987)
REFERENCE 2
AUTHORS Michael,M.C.
TITLE Identification of a DNA structural motif that includes the binding
sites for Sp1, p53 and GA-binding protein
JOURNAL Nucleic Acids Res. 21, 1439-1447 (1993)
COMMENT Three different sizes of cDNA clones coding for the beta 1 subunit
of human alcohol dehydrogenase were isolated from a human cDNA
library constructed in bacteriophage lambda gt 11 and completely
sequenced. They have different polyadenylation sites, respectively.
FEATURES Location/Qualifiers
source 1..2485
/clone="lambda ADH5"
/clone_lib="cDNA library in lambda gt11, provided by
S.L.C.Woo and T.Chandra"
/db_xref="H-InvDB:HIT000100389"
/db_xref="taxon:9606"
/mol_type="mRNA"
/organism="Homo sapiens"
/tissue_type="liver"
CDS 21..1148
/codon_start=1
/product="alcohol dehydrogenase beta 1"
/protein_id="BAA00084.1"
/translation="MSTAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAV
GICRTDDHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCR
VCKNPESNYCLKNDLGNPRGTLQDGTRRFTCRGKPIHHFLGTSTFSQYTVVDENAVAK
IDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAA
RIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTM
MASLLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVA
DFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVLTF"
variation 25..26
/note="deletion in lambda ADH8"
/replace=""
variation 160
/note="g in lambda ADH5, a in lambda ADH8"
/replace="a"
polyA_site 1358
/note="polyadenylation site in lambda ADH5"
variation 1426..1427
/note="deletion in lambda ADH11"
/replace=""
polyA_site 1736
/note="polyadenylation site in lambda ADH8"
polyA_site 2482
/note="polyadenylation site in lambda ADH11"
BASE COUNT 739 a 477 c 535 g 734 t
ORIGIN
1 gcagagaaga cagaaacgac atgagcacag caggaaaagt aatcaaatgc aaagcagctg
61 tgctatggga ggtaaagaaa cccttttcca ttgaggatgt ggaggttgca cctcctaagg
121 cttatgaagt tcgcattaag atggtggctg taggaatctg tcgcacagat gaccacgtgg
181 ttagtggcaa cctggtgacc ccccttcctg tgattttagg ccatgaggca gccggcatcg
241 tggagagtgt tggagaaggg gtgactacag tcaaaccagg tgataaagtc atcccgctct
301 ttactcctca gtgtggaaaa tgcagagttt gtaaaaaccc ggagagcaac tactgcttga
361 aaaatgatct aggcaatcct cgggggaccc tgcaggatgg caccaggagg ttcacctgca
421 gggggaagcc cattcaccac ttccttggca ccagcacctt ctcccagtac acggtggtgg
481 atgagaatgc agtggccaaa attgatgcag cctcgcccct ggagaaagtc tgcctcattg
541 gctgtggatt ctcgactggt tatgggtctg cagttaacgt tgccaaggtc accccaggct
601 ctacctgtgc tgtgtttggc ctgggagggg tcggcctatc tgctgttatg ggctgtaaag
661 cagctggagc agccagaatc attgcggtgg acatcaacaa ggacaaattt gcaaaggcca
721 aagagttggg tgccactgaa tgcatcaacc ctcaagacta caagaaaccc atccaggaag
781 tgctaaagga aatgactgat ggaggtgtgg atttttcgtt tgaagtcatc ggtcggcttg
841 acaccatgat ggcttccctg ttatgttgtc atgaggcatg tggcacaagc gtcatcgtag
901 gggtacctcc tgcttcccag aacctctcaa taaaccctat gctgctactg actggacgca
961 cctggaaggg ggctgtttat ggtggcttta agagtaaaga aggtatccca aaacttgtgg
1021 ctgattttat ggctaagaag ttttcactgg atgcgttaat aacccatgtt ttaccttttg
1081 aaaaaataaa tgaaggattt gacctgcttc actctgggaa aagtatccgt accgtcctga
1141 cgttttgagg caatagagat gccttcccct gtagcagtct tcagcctcct ctaccctaca
1201 agatctggag caacagctag gaaatatcat taattcagct cttcagagat gttatcaata
1261 aattacacat gggggctttc caaagaaatg gaaattgatg ggaaattatt tttcaggaaa
1321 atttaaaatt caagtgagaa gtaaataaag tgttgaacat cagctgggga attgaagcca
1381 acaaaccttc cttcttaacc attctactgt gtcacctttg ccattgagga aaaatattcc
1441 tgtgacttct tgcatttttg gtatcttcat aatctttagt catcgaatcc cagtggaggg
1501 gaccctttta cttgccctga acatacacat gctgggccat tgtgattgaa gtcttctaac
1561 tctgtctcag ttttcactgt cgacattttc ctttttctaa taaaaatgta ccaaatccct
1621 ggggtaaaag ctagggtaag gtaaaggata gactcacatt tacaagtagt gaaggtccaa
1681 gagttctaaa tacaggaaat ttcttaggaa ctcaaataaa atgccccaca ttttactaca
1741 gtaaatggca gtgtttttat gacttttata ctatttcttt atggtcgata tacaattgat
1801 tttttaaaat aatagcagat ttcttgcttc atatgacaaa gcctcaatta ctaattgtaa
1861 aaactgaact attcccagaa tcatgttcaa aaaatctgta atttttgctg atgaaagtgc
1921 ttcattgact aaacagtatt agtttgtggc tataaatgat tatttagatg atgactgaaa
1981 atgtgtataa agtaattaaa agtaatatgg tggctttaag tgtagagatg ggatggcaaa
2041 tgctgtgaat gcagaatgta aaattggtaa ctaagaaatg gcacaaacac cttaagcaat
2101 atattttcct agtagatata tatatacaca tacatatata cacatataca aatgtatatt
2161 tttgcaaaat tgttttcaat ctagaacttt tctattaact accatgtctt aaaatcaagt
2221 ctataatcct agcattagtt taatattttg aatatgtaaa gacctgtgtt aatgctttgt
2281 taatgctttt cccactctca tttgttaatg ctttcccact ctcaggggaa ggatttgcat
2341 tttgagcttt atctctaaat gtgacatgca aagattattc ctggtaaagg aggtagctgt
2401 ctccaaaaat gctattgttg caatatctac attctatttc atattatgaa agaccttaga
2461 cataaagtaa aatagtttat cattt
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