LOCUS CR542271 567 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G0526D for
gene HRAS, v-Ha-ras Harvey rat sarcoma viral oncogene homolog;
complete cds, without stopcodon.
ACCESSION CR542271
VERSION CR542271.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 567)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 567)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G0526D, ORFNo 5086
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G0526D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131097.01L
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_005343 (GI:4885424)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..567
/db_xref="H-InvDB:HIT000269139"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G0526D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>567
/codon_start=1
/gene="HRAS"
/db_xref="GOA:P01112"
/db_xref="H-InvDB:HIT000269139.12"
/db_xref="HGNC:HGNC:5173"
/db_xref="InterPro:IPR001806"
/db_xref="InterPro:IPR005225"
/db_xref="InterPro:IPR020849"
/db_xref="InterPro:IPR027417"
/db_xref="PDB:121P"
/db_xref="PDB:1AA9"
/db_xref="PDB:1AGP"
/db_xref="PDB:1BKD"
/db_xref="PDB:1CLU"
/db_xref="PDB:1CRP"
/db_xref="PDB:1CRQ"
/db_xref="PDB:1CRR"
/db_xref="PDB:1CTQ"
/db_xref="PDB:1GNP"
/db_xref="PDB:1GNQ"
/db_xref="PDB:1GNR"
/db_xref="PDB:1HE8"
/db_xref="PDB:1IAQ"
/db_xref="PDB:1IOZ"
/db_xref="PDB:1JAH"
/db_xref="PDB:1JAI"
/db_xref="PDB:1K8R"
/db_xref="PDB:1LF0"
/db_xref="PDB:1LF5"
/db_xref="PDB:1LFD"
/db_xref="PDB:1NVU"
/db_xref="PDB:1NVV"
/db_xref="PDB:1NVW"
/db_xref="PDB:1NVX"
/db_xref="PDB:1P2S"
/db_xref="PDB:1P2T"
/db_xref="PDB:1P2U"
/db_xref="PDB:1P2V"
/db_xref="PDB:1PLJ"
/db_xref="PDB:1PLK"
/db_xref="PDB:1PLL"
/db_xref="PDB:1Q21"
/db_xref="PDB:1QRA"
/db_xref="PDB:1RVD"
/db_xref="PDB:1WQ1"
/db_xref="PDB:1XCM"
/db_xref="PDB:1XD2"
/db_xref="PDB:1XJ0"
/db_xref="PDB:1ZVQ"
/db_xref="PDB:1ZW6"
/db_xref="PDB:221P"
/db_xref="PDB:2C5L"
/db_xref="PDB:2CE2"
/db_xref="PDB:2CL0"
/db_xref="PDB:2CL6"
/db_xref="PDB:2CL7"
/db_xref="PDB:2CLC"
/db_xref="PDB:2CLD"
/db_xref="PDB:2EVW"
/db_xref="PDB:2GDP"
/db_xref="PDB:2LCF"
/db_xref="PDB:2LWI"
/db_xref="PDB:2N42"
/db_xref="PDB:2N46"
/db_xref="PDB:2Q21"
/db_xref="PDB:2QUZ"
/db_xref="PDB:2RGA"
/db_xref="PDB:2RGB"
/db_xref="PDB:2RGC"
/db_xref="PDB:2RGD"
/db_xref="PDB:2RGE"
/db_xref="PDB:2RGG"
/db_xref="PDB:2UZI"
/db_xref="PDB:2VH5"
/db_xref="PDB:2X1V"
/db_xref="PDB:3DDC"
/db_xref="PDB:3I3S"
/db_xref="PDB:3K8Y"
/db_xref="PDB:3K9L"
/db_xref="PDB:3K9N"
/db_xref="PDB:3KKM"
/db_xref="PDB:3KKN"
/db_xref="PDB:3KUD"
/db_xref="PDB:3L8Y"
/db_xref="PDB:3L8Z"
/db_xref="PDB:3LBH"
/db_xref="PDB:3LBI"
/db_xref="PDB:3LBN"
/db_xref="PDB:3LO5"
/db_xref="PDB:3OIU"
/db_xref="PDB:3OIV"
/db_xref="PDB:3OIW"
/db_xref="PDB:3RRY"
/db_xref="PDB:3RRZ"
/db_xref="PDB:3RS0"
/db_xref="PDB:3RS2"
/db_xref="PDB:3RS3"
/db_xref="PDB:3RS4"
/db_xref="PDB:3RS5"
/db_xref="PDB:3RS7"
/db_xref="PDB:3RSL"
/db_xref="PDB:3RSO"
/db_xref="PDB:3TGP"
/db_xref="PDB:421P"
/db_xref="PDB:4DLR"
/db_xref="PDB:4DLS"
/db_xref="PDB:4DLT"
/db_xref="PDB:4DLU"
/db_xref="PDB:4DLV"
/db_xref="PDB:4DLW"
/db_xref="PDB:4DLX"
/db_xref="PDB:4DLY"
/db_xref="PDB:4DLZ"
/db_xref="PDB:4DST"
/db_xref="PDB:4DSU"
/db_xref="PDB:4EFL"
/db_xref="PDB:4EFM"
/db_xref="PDB:4EFN"
/db_xref="PDB:4G0N"
/db_xref="PDB:4G3X"
/db_xref="PDB:4K81"
/db_xref="PDB:4L9S"
/db_xref="PDB:4L9W"
/db_xref="PDB:4NYI"
/db_xref="PDB:4NYJ"
/db_xref="PDB:4NYM"
/db_xref="PDB:4Q21"
/db_xref="PDB:4RSG"
/db_xref="PDB:4URU"
/db_xref="PDB:4URV"
/db_xref="PDB:4URW"
/db_xref="PDB:4URX"
/db_xref="PDB:4URY"
/db_xref="PDB:4URZ"
/db_xref="PDB:4US0"
/db_xref="PDB:4US1"
/db_xref="PDB:4US2"
/db_xref="PDB:4XVQ"
/db_xref="PDB:4XVR"
/db_xref="PDB:521P"
/db_xref="PDB:5B2Z"
/db_xref="PDB:5B30"
/db_xref="PDB:5E95"
/db_xref="PDB:5P21"
/db_xref="PDB:5VBE"
/db_xref="PDB:5VBZ"
/db_xref="PDB:5WDO"
/db_xref="PDB:5WDP"
/db_xref="PDB:5WDQ"
/db_xref="PDB:5WFO"
/db_xref="PDB:5WFP"
/db_xref="PDB:5WFQ"
/db_xref="PDB:5WFR"
/db_xref="PDB:5WPL"
/db_xref="PDB:5X9S"
/db_xref="PDB:5ZC6"
/db_xref="PDB:621P"
/db_xref="PDB:6AMB"
/db_xref="PDB:6AXG"
/db_xref="PDB:6BVI"
/db_xref="PDB:6BVJ"
/db_xref="PDB:6BVK"
/db_xref="PDB:6BVL"
/db_xref="PDB:6BVM"
/db_xref="PDB:6CUO"
/db_xref="PDB:6CUP"
/db_xref="PDB:6CUR"
/db_xref="PDB:6D55"
/db_xref="PDB:6D56"
/db_xref="PDB:6D59"
/db_xref="PDB:6D5E"
/db_xref="PDB:6D5G"
/db_xref="PDB:6D5H"
/db_xref="PDB:6D5J"
/db_xref="PDB:6D5L"
/db_xref="PDB:6D5M"
/db_xref="PDB:6D5V"
/db_xref="PDB:6D5W"
/db_xref="PDB:6DZH"
/db_xref="PDB:6Q21"
/db_xref="PDB:721P"
/db_xref="PDB:821P"
/db_xref="UniProtKB/Swiss-Prot:P01112"
/protein_id="CAG47067.1"
/translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQV
VIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKR
VKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLV
REIRQHKLRKLNPPDESGPGCMSCKCVLS"
BASE COUNT 125 a 151 c 186 g 105 t
ORIGIN
1 atgacggaat ataagctggt ggtggtgggc gccggcggtg tgggcaagag tgcgctgacc
61 atccagctga tccagaacca ctttgtggac gaatacgacc ccactataga ggattcctac
121 cggaagcagg tggtcattga tggggagacg tgcctgttgg acatcctgga taccgccggc
181 caggaggagt acagcgccat gcgggaccag tacatgcgca ccggggaggg cttcctgtgt
241 gtgtttgcca tcaacaacac caagtctttt gaggacatcc accagtacag ggagcagatc
301 aaacgggtga aggactcgga tgacgtgccc atggtgctgg tggggaacaa gtgtgacctg
361 gctgcacgca ctgtggaatc tcggcaggct caggacctcg cccgaagcta cggcatcccc
421 tacatcgaga cctcggccaa gacccggcag ggagtggagg atgccttcta cacgttggtg
481 cgtgagatcc ggcagcacaa gctgcggaag ctgaaccctc ctgatgagag tggccccggc
541 tgcatgagct gcaagtgtgt gctctcc
//