LOCUS CR542264 1062 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G0126D for
gene KCNAB2, potassium voltage-gated channel, shaker-related
subfamily, beta member 2; complete cds, incl. stopcodon.
ACCESSION CR542264
VERSION CR542264.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1062)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1062)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G0126D, ORFNo 5068
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G0126D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_172130 (GI:27436968)
we found AA exchange(s) at position (first base of changed
triplet):
397(leu->arg) 616(phe->ser)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1062
/db_xref="H-InvDB:HIT000269132"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G0126D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1062
/codon_start=1
/gene="KCNAB2"
/db_xref="GOA:Q6FG44"
/db_xref="H-InvDB:HIT000269132.13"
/db_xref="InterPro:IPR005399"
/db_xref="InterPro:IPR005401"
/db_xref="InterPro:IPR005983"
/db_xref="InterPro:IPR023210"
/db_xref="InterPro:IPR036812"
/db_xref="UniProtKB/TrEMBL:Q6FG44"
/protein_id="CAG47060.1"
/translation="MYPESTTGSPARLSLRQTGSPGMIYRNLGKSGLRVSCLGLGTWV
TFGGQITDEMAEQLMTLAYDNGINLFDTAEVYAAGKAEVVLGNIIKKKGWRRSSLVIT
TKIFWGGKAETERGLSRKHIIEGLKASLERRQLEYVDVVFANRPDPNTPMEETVRAMT
HVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLTPPICEQAEYHMSQREKVEVQLPEL
FHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLKDKILSEEGRRQQAKLK
ELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNADQLMENIGAIQVLPKLSSSII
HEIDSILGNKPYSKKDYRS"
BASE COUNT 245 a 305 c 322 g 190 t
ORIGIN
1 atgtatccag aatcaacgac gggctccccg gctcggctct cgctgcggca gacgggctcc
61 cccgggatga tctacaggaa cctgggcaag tctggcctgc gggtctcctg cctgggactt
121 ggaacatggg tgaccttcgg aggccagatc accgatgaga tggcagagca gctcatgacc
181 ttggcctatg ataatggcat caacctcttc gatacagcag aagtctacgc agccggcaag
241 gctgaagtgg tactgggaaa catcattaag aagaaaggat ggaggcggtc cagcctcgtc
301 atcaccacca agatcttctg gggcggaaag gcggagacgg agcggggcct gtccaggaag
361 cacataatcg aaggtctgaa agcttccctg gagcgacggc agctggagta cgtggatgtg
421 gtgtttgcca accgcccgga ccccaacacc ccgatggaag agaccgtccg cgccatgacc
481 cacgtcatca accaggggat ggccatgtac tggggcacgt cacgctggag ctccatggag
541 atcatggagg cctactccgt ggcccggcag ttcaacctga ccccgcccat ctgcgagcag
601 gctgagtacc acatgtccca gcgtgagaaa gtggaggtgc agctgccgga gctgttccac
661 aagataggag tgggcgccat gacctggtcc cctctggcct gtggcattgt ttctggcaag
721 tacgacagtg gcatcccacc ctactcaaga gcctccttga agggctacca gtggctgaag
781 gacaagatcc tcagtgagga gggccggcgc cagcaagcca agctgaagga gctgcaggcc
841 atcgccgagc gcctgggctg caccctgccc cagctggcca tagcctggtg cctgaggaat
901 gagggagtca gctccgtgct cctgggggcc tccaatgcgg accagctcat ggagaacatt
961 ggggcaatac aggtccttcc gaaactgtcg tcttccatta tccacgagat tgatagtatt
1021 ttgggcaata aaccctacag caaaaaggac tacagatcct aa
//