LOCUS CR542259 945 bp mRNA linear HUM 17-APR-2005 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0926D for gene TACSTD1, tumor-associated calcium signal transducer 1; complete cds, incl. stopcodon. ACCESSION CR542259 VERSION CR542259.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 945) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 945) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834D0926D, ORFNo 5052 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0926D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence M32306 we found AA exchange(s) at position (first base of changed triplet): 907(lys->arg) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..945 /db_xref="H-InvDB:HIT000269127" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834D0926D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..945 /codon_start=1 /gene="TACSTD1" /db_xref="GOA:P16422" /db_xref="H-InvDB:HIT000269127.12" /db_xref="HGNC:HGNC:11529" /db_xref="InterPro:IPR000716" /db_xref="InterPro:IPR036857" /db_xref="InterPro:IPR041630" /db_xref="PDB:4MZV" /db_xref="PDB:6I07" /db_xref="UniProtKB/Swiss-Prot:P16422" /protein_id="CAG47055.1" /translation="MAPPQVLAFGLLLAAATATFAAAQEECVCENYKLAVNCFVNNNR QCQCTSVGAQNTVICSKLAAKCLVMKAEMNGSKLGRRAKPEGALQNNDGLYDPDCDES GLFKAKQCNGTSMCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKAREKPYDSK SLRTALQKEITTRYQLDPKFITSILYENNVITIDLVQNSSQKTQNDVDIADVAYYFEK DVKGESLFHSKKMDLTVNGEQLDLDPGQTLIYYVDEKAPEFSMQGLKAGVIAVIVVVV IAVVAGIVVLVISRKKRMAKYEKAEIREMGEMHRELNA" BASE COUNT 287 a 168 c 242 g 248 t ORIGIN 1 atggcgcccc cgcaggtcct cgcgttcggg cttctgcttg ccgcggcgac ggcgactttt 61 gccgcagctc aggaagaatg tgtctgtgaa aactacaagc tggccgtaaa ctgctttgtg 121 aataataatc gtcaatgcca gtgtacttca gttggtgcac aaaatactgt catttgctca 181 aagctggctg ccaaatgttt ggtgatgaag gcagaaatga atggctcaaa acttgggaga 241 agagcaaaac ctgaaggggc cctccagaac aatgatgggc tttatgatcc tgactgcgat 301 gagagcgggc tctttaaggc caagcagtgc aacggcacct ccatgtgctg gtgtgtgaac 361 actgctgggg tcagaagaac agacaaggac actgaaataa cctgctctga gcgagtgaga 421 acctactgga tcatcattga actaaaacac aaagcaagag aaaaacctta tgatagtaaa 481 agtttgcgga ctgcacttca gaaggagatc acaacgcgtt atcaactgga tccaaaattt 541 atcacgagta ttttgtatga gaataatgtt atcactattg atctggttca aaattcttct 601 caaaaaactc agaatgatgt ggacatagct gatgtggctt attattttga aaaagatgtt 661 aaaggtgaat ccttgtttca ttctaagaaa atggacctga cagtaaatgg ggaacaactg 721 gatctggatc ctggtcaaac tttaatttat tatgttgatg aaaaagcacc tgaattctca 781 atgcagggtc taaaagctgg tgttattgct gttattgtgg ttgtggtgat agcagttgtt 841 gctggaattg ttgtgctggt tatttccaga aagaagagaa tggcaaagta tgagaaggct 901 gagataaggg agatgggtga gatgcatagg gaactcaatg cataa //