LOCUS CR542254 1326 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0726D for
gene ETS1, v-ets erythroblastosis virus E26 oncogene homolog 1
(avian); complete cds, incl. stopcodon.
ACCESSION CR542254
VERSION CR542254.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1326)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1326)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0726D, ORFNo 5040
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0726D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131104.01X
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_005238 (GI:41393580)
we found AA exchange(s) at position (first base of changed
triplet):
1012(trp->arg)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1326
/db_xref="H-InvDB:HIT000269122"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0726D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1326
/codon_start=1
/gene="ETS1"
/db_xref="GOA:Q6FG54"
/db_xref="H-InvDB:HIT000269122.15"
/db_xref="InterPro:IPR000418"
/db_xref="InterPro:IPR003118"
/db_xref="InterPro:IPR013761"
/db_xref="InterPro:IPR016311"
/db_xref="InterPro:IPR036388"
/db_xref="InterPro:IPR036390"
/db_xref="InterPro:IPR041886"
/db_xref="UniProtKB/TrEMBL:Q6FG54"
/protein_id="CAG47050.1"
/translation="MKAAVDLKPTLTIIKTEKVDLELFPSPDMECADVPLLTPSSKEM
MSQALKATFSGFTKEQQRLGIPKDPRQWTETHVRDWVMWAVNEFSLKGVDFQKFCMNG
AALCALGKDCFLELAPDFVGDILWEHLEILQKEDVKPYQVNGVNPAYPESRYTSDYFI
SYGIEHAQCVPPSEFSEPSFITESYQTLHPISSEELLSLKYENDYPSVILRDPLQTDT
LQNDYFAIKQEVVTPDNMCMGRTSRGKLGGQDSFESIESYDSCDRLTQSWSSQSSFNS
LQRVPSYDSFDSEDYPAALPNHKPKGTFKDYVRDRADLNKDKPVIPAAALAGYTGSGP
IQLRQFLLELLTDKSCQSFISWTGDGWEFKLSDPDEVARRWGKRKNKPKMNYEKLSRG
LRYYYDKNIIHKTAGKRYVYRFVCDLQSLLGYTPEELHAMLDVKPDADE"
BASE COUNT 356 a 357 c 324 g 289 t
ORIGIN
1 atgaaggcgg ccgtcgatct caagccgact ctcaccatca tcaagacgga aaaagtcgat
61 ctggagcttt tcccctcccc ggatatggaa tgtgcagatg tcccactatt aactccaagc
121 agcaaagaaa tgatgtctca agcattaaaa gctactttca gtggtttcac taaagaacag
181 caacgactgg ggatcccaaa agacccccgg cagtggacag aaacccatgt tcgggactgg
241 gtgatgtggg ctgtgaatga attcagcctg aaaggtgtag acttccagaa gttctgtatg
301 aatggagcag ccctctgcgc cctgggtaaa gactgctttc tcgagctggc cccagacttt
361 gttggggaca tcttatggga acatctagag atcctgcaga aagaggatgt gaaaccatat
421 caagttaatg gagtcaaccc agcctatcca gaatcccgct atacctcgga ttacttcatt
481 agctatggta ttgagcatgc ccagtgtgtt ccaccatcgg agttctcaga gcccagcttc
541 atcacagagt cctatcagac gctccatccc atcagctcgg aagagctcct ctccctcaag
601 tatgagaatg actacccctc ggtcattctc cgagaccctc tccagacaga caccttgcag
661 aatgactact ttgctatcaa acaagaagtc gtcaccccag acaacatgtg catggggagg
721 accagtcgtg gtaaactcgg gggccaggac tcttttgaaa gcatagagag ctacgatagt
781 tgtgatcgcc tcacccagtc ctggagcagc cagtcatctt tcaacagcct gcagcgtgtt
841 ccctcctatg acagcttcga ctcagaggac tatccggctg ccctgcccaa ccacaagccc
901 aagggcacct tcaaggacta tgtgcgggac cgtgctgacc tcaataagga caagcctgtc
961 attcctgctg ctgccctagc tggctacaca ggcagtggac caatccagct acggcagttt
1021 cttctggaat tactcactga taaatcctgt cagtctttta tcagctggac aggagatggc
1081 tgggaattca aactttctga cccagatgag gtggccagga gatggggaaa gaggaaaaac
1141 aaacctaaga tgaattatga gaaactgagc cgtggcctac gctactatta cgacaaaaac
1201 atcatccaca agacagcggg gaaacgctac gtgtaccgct ttgtgtgtga cctgcagagc
1261 ctgctggggt acacccctga ggagctgcac gccatgctgg acgtcaagcc agatgccgac
1321 gagtga
//