LOCUS CR542249 1137 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0526D for
gene CDC37, CDC37 cell division cycle 37 homolog (S. cerevisiae);
complete cds, incl. stopcodon.
ACCESSION CR542249
VERSION CR542249.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1137)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1137)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A0526D, ORFNo 5025
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0526D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131093.01X
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_007065 (GI:39995072)
we found AA exchange(s) at position (first base of changed
triplet):
943(gln->leu)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1137
/db_xref="H-InvDB:HIT000269117"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A0526D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1137
/codon_start=1
/gene="CDC37"
/db_xref="GOA:Q6FG59"
/db_xref="H-InvDB:HIT000269117.12"
/db_xref="InterPro:IPR004918"
/db_xref="InterPro:IPR013855"
/db_xref="InterPro:IPR013873"
/db_xref="InterPro:IPR013874"
/db_xref="InterPro:IPR038189"
/db_xref="UniProtKB/TrEMBL:Q6FG59"
/protein_id="CAG47045.1"
/translation="MVDYSVWDHIEVSDDEDETHPNIDTASLFRWRHQARVERMEQFQ
KEKEELDRGCRECKRKVAECQRKLKELEVAEGGKAELERLQAEAQQLRKEERSWEQKL
EEMRKKEKSMPWNVDTLSKDGFSKSMVNTKPEKTEEDSEEVREQKHKTFVEKYEKQIK
HFGMLRRWDDSQKYLSDNVHLVCEETANYLVIWCIDLEVEEKCALMEQVAHQTIVMQF
ILELAKSLKVDPRACFRQFFTKIKTADRQYMEGFNDELEAFKERVRGRAKLRIEKAMK
EYEEEERKKRLGPGGLDPVEVYESLPEELQKCFDVKDVLMLQDAISKMDPTDAKYHMQ
RCIDSGLWVPNSKASEAKEGEEAGPGDPLLEAVPKTGDEKDVSV"
BASE COUNT 298 a 283 c 389 g 167 t
ORIGIN
1 atggtggact acagcgtgtg ggaccacatt gaggtgtctg atgatgaaga cgagacgcac
61 cccaacatcg acacggccag tctcttccgc tggcggcatc aggcccgggt ggaacgcatg
121 gagcagttcc agaaggagaa ggaggaactg gacaggggct gccgcgagtg caagcgcaag
181 gtggccgagt gccagaggaa actgaaggag ctggaggtgg ccgagggcgg caaggcagag
241 ctggagcgcc tgcaggccga ggcacagcag ctgcgcaagg aggagcggag ctgggagcag
301 aagctggagg agatgcgcaa gaaggagaag agcatgccct ggaacgtgga cacgctcagc
361 aaagacggct tcagcaagag catggtaaat accaagcccg agaagacgga ggaggactca
421 gaggaggtga gggagcagaa acacaagacc ttcgtggaaa aatacgagaa acagatcaag
481 cactttggca tgcttcgccg ctgggatgac agccaaaagt acctgtcaga caacgtccac
541 ctggtgtgcg aggagacagc caattacctg gtcatttggt gcattgacct agaggtggag
601 gagaaatgtg cactcatgga gcaggtggcc caccagacaa tcgtcatgca atttatcctg
661 gagctggcca agagcctaaa ggtggacccc cgggcctgct tccggcagtt cttcactaag
721 attaagacag ccgatcgcca gtacatggag ggcttcaacg acgagctgga agccttcaag
781 gagcgtgtgc ggggccgtgc caagctgcgc atcgagaagg ccatgaagga gtacgaggag
841 gaggagcgca agaagcggct cggccccggc ggcctggacc ccgtcgaggt ctacgagtcc
901 ctccctgagg aactccagaa gtgcttcgat gtgaaggacg tgctgatgct gcaggacgcc
961 atcagcaaga tggaccccac cgacgcaaag taccacatgc agcgctgcat tgactctggc
1021 ctctgggtcc ccaactctaa ggccagcgag gccaaggagg gagaggaggc aggtcctggg
1081 gacccattac tggaagctgt tcccaagacg ggcgatgaga aggatgtcag tgtgtga
//