LOCUS CR542232 351 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0525D for gene RPL34, ribosomal protein L34; complete cds, without stopcodon. ACCESSION CR542232 VERSION CR542232.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 351) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 351) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834E0525D, ORFNo 4974 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0525D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. Clone name at Harvard Institute of Proteomics (www.hip.harvard.edu): FLH131136.01L This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_033625 (GI:16117788) we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..351 /db_xref="H-InvDB:HIT000269100" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834E0525D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>351 /codon_start=1 /gene="RPL34" /db_xref="GOA:P49207" /db_xref="H-InvDB:HIT000269100.12" /db_xref="HGNC:HGNC:10340" /db_xref="InterPro:IPR008195" /db_xref="InterPro:IPR018065" /db_xref="InterPro:IPR038562" /db_xref="PDB:4UG0" /db_xref="PDB:4V6X" /db_xref="PDB:5AJ0" /db_xref="PDB:5LKS" /db_xref="PDB:5T2C" /db_xref="PDB:6EK0" /db_xref="PDB:6IP5" /db_xref="PDB:6IP6" /db_xref="PDB:6IP8" /db_xref="PDB:6OLE" /db_xref="PDB:6OLF" /db_xref="PDB:6OLG" /db_xref="PDB:6OLI" /db_xref="PDB:6OLZ" /db_xref="PDB:6OM0" /db_xref="PDB:6OM7" /db_xref="PDB:6QZP" /db_xref="UniProtKB/Swiss-Prot:P49207" /protein_id="CAG47028.1" /translation="MVQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKS ACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKI VVKVLKAQAQSQKAK" BASE COUNT 104 a 72 c 88 g 87 t ORIGIN 1 atggtccagc gtttgacata ccgacgtagg ctttcctaca atacagcctc taacaaaact 61 aggctgtccc gaacccctgg taatagaatt gtttaccttt ataccaagaa ggttgggaaa 121 gcaccaaaat ctgcatgtgg tgtgtgccca ggcagacttc gaggggttcg tgctgtaaga 181 cctaaagttc ttatgagatt gtccaaaaca aagaaacatg tcagcagggc ctatggtggt 241 tccatgtgtg ctaaatgtgt tcgtgacagg atcaagcgtg ctttccttat cgaggagcag 301 aaaatcgttg tgaaagtgtt gaaggcacaa gcacagagtc agaaagctaa a //