LOCUS CR542229 1344 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G0225D for
gene MAP2K5, mitogen-activated protein kinase kinase 5; complete
cds, without stopcodon.
ACCESSION CR542229
VERSION CR542229.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1344)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1344)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G0225D, ORFNo 4962
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G0225D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_145160 (GI:21729894)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1344
/db_xref="H-InvDB:HIT000269097"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G0225D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1344
/codon_start=1
/gene="MAP2K5"
/db_xref="GOA:Q13163"
/db_xref="H-InvDB:HIT000269097.13"
/db_xref="HGNC:HGNC:6845"
/db_xref="InterPro:IPR000270"
/db_xref="InterPro:IPR000719"
/db_xref="InterPro:IPR008271"
/db_xref="InterPro:IPR011009"
/db_xref="InterPro:IPR017441"
/db_xref="InterPro:IPR034851"
/db_xref="PDB:2NPT"
/db_xref="PDB:2O2V"
/db_xref="PDB:4IC7"
/db_xref="UniProtKB/Swiss-Prot:Q13163"
/protein_id="CAG47025.1"
/translation="MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDV
LDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPL
QIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSLPSNSLKKSSAELKKILANGQ
MNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIMSELEILYK
CDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWS
LKILHRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYG
IHSDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPF
VHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCRALEERRSQQGPP"
BASE COUNT 386 a 266 c 344 g 348 t
ORIGIN
1 atgctgtggc tagcccttgg cccctttcct gccatggaga accaggtgct ggtaattcgc
61 atcaagatcc caaatagtgg cgcggtggac tggacagtgc actccgggcc gcagttactc
121 ttcagggatg tgctggatgt gataggccag gttctgcctg aagcaacaac tacagcattt
181 gaatatgaag atgaagatgg tgatcgaatt acagtgagaa gtgatgagga aatgaaggca
241 atgctgtcat attattattc cacagtaatg gaacagcaag taaatggaca gttaatagag
301 cctctgcaga tatttccaag agcctgcaag cctcctgggg aacggaacat acatggcctg
361 aaggtgaata ctcgggccgg accctctcaa cacagcagcc cagcagtctc agattcactt
421 ccaagcaata gcttaaagaa gtcttctgct gaactgaaaa aaatactagc caatggccag
481 atgaatgaac aagacatacg atatcgggac actcttggtc atggcaacgg aggcacagtc
541 tacaaagcat atcatgtccc gagtgggaaa atattagctg taaaggtcat actactagat
601 attacactgg aacttcagaa gcaaattatg tctgaattgg aaattcttta taagtgcgat
661 tcatcatata tcattggatt ttatggagca ttttttgtag aaaacaggat ttcaatatgt
721 acagaattca tggatggggg atctttggat gtatatagga aaatgccaga acatgtcctt
781 ggaagaattg cagtagcagt tgttaaaggc cttacttatt tgtggagttt aaagatttta
841 catagagatg tgaagccctc caatatgcta gtaaacacaa gaggacaggt taagctgtgt
901 gattttggag ttagcactca gctggtgaat tctatagcca agacgtatgt tggaacaaat
961 gcttatatgg cgcctgaaag gatttcaggg gagcagtatg gaattcattc tgatgtctgg
1021 agcttaggaa tctcttttat ggagcttgct cttgggaggt ttccatatcc tcagattcag
1081 aaaaaccagg gatctttaat gcctctccag cttctgcagt gcattgttga tgaggattcg
1141 cccgtccttc cagttggaga gttctcggag ccatttgtac atttcatcac tcagtgtatg
1201 cgaaaacagc caaaagaaag gccagcacct gaagaattga tgggccaccc gttcatcgtg
1261 cagttcaatg atggaaatgc cgccgtggtg tccatgtggg tgtgccgggc gctggaggag
1321 aggcggagcc agcaggggcc cccg
//