LOCUS CR542227 1293 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0225D for
gene STK24, serine/threonine kinase 24 (STE20 homolog, yeast);
complete cds, without stopcodon.
ACCESSION CR542227
VERSION CR542227.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1293)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1293)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0225D, ORFNo 4960
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0225D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131127.01L
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence BC035578
we found AA exchange(s) at position (first base of changed
triplet):
487(phe->ile)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1293
/db_xref="H-InvDB:HIT000269095"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0225D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1293
/codon_start=1
/gene="STK24"
/db_xref="GOA:Q6FG81"
/db_xref="H-InvDB:HIT000269095.13"
/db_xref="InterPro:IPR000719"
/db_xref="InterPro:IPR011009"
/db_xref="InterPro:IPR017441"
/db_xref="UniProtKB/TrEMBL:Q6FG81"
/protein_id="CAG47023.1"
/translation="MAHSPVQSGLPGMQNLKADPEELFTKLEKIGKGSFGEVFKGIDN
RTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYVTKYYGSYLKDTKLWIIMEYL
GGGSALDLLEPGPLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKL
ADIGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGEPP
HSELHPMKVLFLIPKNNPPTLEGNYSKPLKEFVEACLNKEPSFRPTAKELLKHKFILR
NAKKTSYLTELIDRYKRWKAEQSHDDSSSEDSDAETDGQASGGSDSGDWIFTIREKDP
KNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEEL
RGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH"
BASE COUNT 384 a 303 c 337 g 269 t
ORIGIN
1 atggctcact ccccggtgca gtcgggcctg cccggcatgc agaacctaaa ggcagaccca
61 gaagagcttt ttacaaaact agagaaaatt gggaagggct cctttggaga ggtgttcaaa
121 ggcattgaca atcggactca gaaagtggtt gccataaaga tcattgatct ggaagaagct
181 gaagatgaga tagaggacat tcaacaagaa atcacagtgc tgagtcagtg tgacagtcca
241 tatgtaacca aatattatgg atcctatctg aaggatacaa aattatggat aataatggaa
301 tatcttggtg gaggctccgc actagatcta ttagaacctg gcccattaga tgaaacccag
361 atcgctacta tattaagaga aatactgaaa ggactcgatt atctccattc ggagaagaaa
421 atccacagag acattaaagc ggccaacgtc ctgctgtctg agcatggcga ggtgaagctg
481 gcggacattg gcgtggctgg ccagctgaca gacacccaga tcaaaaggaa caccttcgtg
541 ggcaccccat tctggatggc acccgaggtc atcaaacagt cggcctatga ctcgaaggca
601 gacatctggt ccctgggcat aacagctatt gaacttgcaa gaggggaacc acctcattcc
661 gagctgcacc ccatgaaagt tttattcctc attccaaaga acaacccacc gacgttggaa
721 ggaaactaca gtaaacccct caaggagttt gtggaggcct gtttgaataa ggagccgagc
781 tttagaccca ctgctaagga gttattgaag cacaagttta tactacgcaa tgcaaagaaa
841 acttcctact tgaccgagct catcgacagg tacaagagat ggaaggccga gcagagccat
901 gacgactcga gctccgagga ttccgacgcg gaaacagatg gccaagcctc ggggggcagt
961 gattctgggg actggatctt cacaatccga gaaaaagatc ccaagaatct cgagaatgga
1021 gctcttcagc catcggactt ggacagaaat aagatgaaag acatcccaaa gaggcctttc
1081 tctcagtgtt tatctacaat tatttctcct ctgtttgcag agttgaagga gaagagccag
1141 gcgtgcggag ggaacttggg gtccattgaa gagctgcgag gggccatcta cctagcggag
1201 gaggcgtgcc ctggcatctc cgacaccatg gtggcccagc tcgtgcagcg gctccagaga
1261 tactctctaa gtggtggagg aacttcatcc cac
//