LOCUS CR542221 903 bp mRNA linear HUM 29-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0925D for
gene TCEA1, transcription elongation factor A (SII), 1; complete
cds, without stopcodon.
ACCESSION CR542221
VERSION CR542221.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 903)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 903)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0925D, ORFNo 4944
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0925D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131150.01L
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_006756 (GI:45439353)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..903
/db_xref="H-InvDB:HIT000269089"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0925D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>903
/codon_start=1
/gene="TCEA1"
/db_xref="GOA:P23193"
/db_xref="H-InvDB:HIT000269089.14"
/db_xref="HGNC:HGNC:11612"
/db_xref="InterPro:IPR001222"
/db_xref="InterPro:IPR003617"
/db_xref="InterPro:IPR003618"
/db_xref="InterPro:IPR006289"
/db_xref="InterPro:IPR017923"
/db_xref="InterPro:IPR035100"
/db_xref="InterPro:IPR035441"
/db_xref="InterPro:IPR036575"
/db_xref="PDB:1TFI"
/db_xref="PDB:3NDQ"
/db_xref="PDB:5IY6"
/db_xref="PDB:5IY7"
/db_xref="PDB:5IY8"
/db_xref="PDB:5IYA"
/db_xref="PDB:5IYB"
/db_xref="PDB:5IYC"
/db_xref="PDB:6O9L"
/db_xref="UniProtKB/Swiss-Prot:P23193"
/protein_id="CAG47017.1"
/translation="MEDEVVRFAKKMDKMVQKKNAAGALDLLKELKNIPMTLELLQST
RIGMSVNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTEKDLDEKKKEPAITSQNSPE
AREESTSSGNVSNRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYIA
IGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPD
LFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQV
QTRSADEPMTTFVVCNECGNRWKFC"
BASE COUNT 322 a 157 c 220 g 204 t
ORIGIN
1 atggaggacg aagtggtccg ctttgccaag aagatggaca agatggtgca gaagaagaac
61 gcggctggag cattggattt gctaaaggag cttaagaata ttcctatgac cctggaatta
121 ctgcagtcca caagaatcgg aatgtcagtt aatgctattc gcaagcagag tacagatgag
181 gaagttacat ctttggcaaa gtctctcatc aaatcctgga aaaaattatt agatgggcca
241 tcaactgaga aagaccttga cgaaaagaag aaagaacctg caattacatc gcagaacagc
301 cctgaggcaa gagaagaaag tacttccagc ggcaatgtaa gcaacagaaa ggatgagaca
361 aatgctcgag atacttatgt ttcatccttt cctcgggcac caagcacttc tgattctgtg
421 cggttgaagt gtagggagat gcttgctgca gctcttcgaa caggggatga ctacattgca
481 attggagctg atgaggaaga attaggatct caaattgaag aagctatata tcaagaaata
541 aggaatacag acatgaaata caaaaataga gtacgaagta ggatatcaaa tcttaaagat
601 gcaaaaaatc caaatttaag aaaaaatgtc ctctgtggga atattcctcc tgacttattt
661 gctagaatga cagcagagga aatggctagt gatgagctga aagagatgcg gaaaaacttg
721 accaaagaag ccatcagaga gcatcagatg gccaagactg gtgggaccca gactgacttg
781 ttcacatgtg gcaaatgtaa aaagaagaat tgcacttaca cacaggtaca aacccgtagt
841 gctgatgaac caatgacaac atttgttgtc tgtaatgaat gtggaaatcg atggaagttc
901 tgt
//