LOCUS CR542209 345 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0225D for gene RPLP1, ribosomal protein, large, P1; complete cds, incl. stopcodon. ACCESSION CR542209 VERSION CR542209.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 345) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 345) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834A0225D, ORFNo 4912 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0225D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. Clone name at Harvard Institute of Proteomics (www.hip.harvard.edu): FLH131124.01X This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_001003 (GI:16905511) we found AA exchange(s) at position (first base of changed triplet): 25(cys->arg) 184(asn->asp) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..345 /db_xref="H-InvDB:HIT000269077" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834A0225D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..345 /codon_start=1 /gene="RPLP1" /db_xref="GOA:Q6FG99" /db_xref="H-InvDB:HIT000269077.13" /db_xref="InterPro:IPR027534" /db_xref="InterPro:IPR038716" /db_xref="UniProtKB/TrEMBL:Q6FG99" /protein_id="CAG47005.1" /translation="MASVSELARIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWP GLFAKALANVNIGSLICDVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESE ESDDDMGFGLFD" BASE COUNT 77 a 91 c 99 g 78 t ORIGIN 1 atggcctctg tctccgagct cgcccgcatc tactcggccc tcattctgca cgacgatgag 61 gtgacagtca cggaggataa gatcaatgcc ctcattaaag cagccggtgt aaatgttgag 121 cctttttggc ctggcttgtt tgcaaaggcc ctggccaacg tcaacattgg gagcctcatc 181 tgcgatgtag gggccggtgg acctgctcca gcagctggtg ctgcaccagc aggaggtcct 241 gccccctcca ctgctgctgc tccagctgag gagaagaaag tggaagcaaa gaaagaagaa 301 tccgaggagt ctgatgatga catgggcttt ggtctttttg actaa //