LOCUS CR542186 324 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G0424D for gene TBCA, tubulin-specific chaperone a; complete cds, without stopcodon. ACCESSION CR542186 VERSION CR542186.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 324) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 324) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834G0424D, ORFNo 4872 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G0424D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_004607 (GI:4759211) we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..324 /db_xref="H-InvDB:HIT000269055" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834G0424D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>324 /codon_start=1 /gene="TBCA" /db_xref="GOA:Q6FGD7" /db_xref="H-InvDB:HIT000269055.12" /db_xref="InterPro:IPR004226" /db_xref="InterPro:IPR036126" /db_xref="UniProtKB/TrEMBL:Q6FGD7" /protein_id="CAG46983.1" /translation="MADPRVRQIKIKTGVVKRLVKEKVMYEKEAKQQEEKIEKMRAED GENYDIKKQAEILQESRMMIPDCQRRLEAAYLDLQRILENEKDLEEAEEYKEARLVLD SVKLEA" BASE COUNT 128 a 49 c 87 g 60 t ORIGIN 1 atggccgatc ctcgcgtgag acagatcaag atcaagaccg gcgtggtgaa gcggttggtc 61 aaagaaaaag tgatgtatga aaaagaggca aaacaacaag aagaaaagat tgaaaaaatg 121 agagctgaag acggtgaaaa ttatgacatt aaaaagcagg cagagatcct acaagaatcc 181 aggatgatga tcccagattg ccagcgcagg ttggaagccg catatttgga tcttcaacgg 241 atactagaaa atgaaaaaga cttggaagaa gctgaggaat ataaagaagc acgtttagta 301 ctggattcag tgaagttaga agcc //