LOCUS CR542170 327 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0824D for
gene TBCA, tubulin-specific chaperone a; complete cds, incl.
stopcodon.
ACCESSION CR542170
VERSION CR542170.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 327)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 327)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A0824D, ORFNo 4831
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0824D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_004607 (GI:4759211)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..327
/db_xref="H-InvDB:HIT000269039"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A0824D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..327
/codon_start=1
/gene="TBCA"
/db_xref="GOA:Q6FGD7"
/db_xref="H-InvDB:HIT000269039.12"
/db_xref="InterPro:IPR004226"
/db_xref="InterPro:IPR036126"
/db_xref="UniProtKB/TrEMBL:Q6FGD7"
/protein_id="CAG46967.1"
/translation="MADPRVRQIKIKTGVVKRLVKEKVMYEKEAKQQEEKIEKMRAED
GENYDIKKQAEILQESRMMIPDCQRRLEAAYLDLQRILENEKDLEEAEEYKEARLVLD
SVKLEA"
BASE COUNT 129 a 49 c 88 g 61 t
ORIGIN
1 atggccgatc ctcgcgtgag acagatcaag atcaagaccg gcgtggtgaa gcggttggtc
61 aaagaaaaag tgatgtatga aaaagaggca aaacaacaag aagaaaagat tgaaaaaatg
121 agagctgaag acggtgaaaa ttatgacatt aaaaagcagg cagagatcct acaagaatcc
181 aggatgatga tcccagattg ccagcgcagg ttggaagccg catatttgga tcttcaacgg
241 atactagaaa atgaaaaaga cttggaagaa gctgaggaat ataaagaagc acgtttagta
301 ctggattcag tgaagttaga agcctga
//