LOCUS CR542165 321 bp mRNA linear HUM 29-JUN-2004 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0524D for gene GLRX, glutaredoxin (thioltransferase); complete cds, incl. stopcodon. ACCESSION CR542165 VERSION CR542165.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 321) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 321) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834D0524D, ORFNo 4821 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0524D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_002064 (GI:4504024) we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..321 /db_xref="H-InvDB:HIT000269034" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834D0524D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..321 /codon_start=1 /gene="GLRX" /db_xref="GOA:P35754" /db_xref="H-InvDB:HIT000269034.13" /db_xref="HGNC:HGNC:4330" /db_xref="InterPro:IPR002109" /db_xref="InterPro:IPR011767" /db_xref="InterPro:IPR011899" /db_xref="InterPro:IPR014025" /db_xref="InterPro:IPR036249" /db_xref="PDB:1B4Q" /db_xref="PDB:1JHB" /db_xref="PDB:4RQR" /db_xref="UniProtKB/Swiss-Prot:P35754" /protein_id="CAG46962.1" /translation="MAQEFVNCKIQPGKVVVFIKPTCPYCRRAQEILSQLPIKQGLLE FVDITATNHTNEIQDYLQQLTGARTVPRVFIGKDCIGGCSDLVSLQQSGELLTRLKQI GALQ" BASE COUNT 89 a 74 c 84 g 74 t ORIGIN 1 atggctcaag agtttgtgaa ctgcaaaatc cagcctggga aggtggttgt gttcatcaag 61 cccacctgcc cgtactgcag gagggcccaa gagatcctca gtcaattgcc catcaaacaa 121 gggcttctgg aatttgtcga tatcacagcc accaaccaca ctaacgagat tcaagattat 181 ttgcaacagc tcacgggagc aagaacggtg cctcgagtct ttattggtaa agattgtata 241 ggcggatgca gtgatctagt ctctttgcaa cagagtgggg aactgctgac gcggctaaag 301 cagattggag ctctgcagta a //