LOCUS CR542154 300 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G1223D for gene VAMP3, vesicle-associated membrane protein 3 (cellubrevin); complete cds, without stopcodon. ACCESSION CR542154 VERSION CR542154.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 300) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 300) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834G1223D, ORFNo 4798 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G1223D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_004781 (GI:42544205) we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..300 /db_xref="H-InvDB:HIT000269023" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834G1223D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>300 /codon_start=1 /gene="VAMP3" /db_xref="GOA:Q6FGG2" /db_xref="H-InvDB:HIT000269023.13" /db_xref="InterPro:IPR001388" /db_xref="InterPro:IPR016444" /db_xref="UniProtKB/TrEMBL:Q6FGG2" /protein_id="CAG46951.1" /translation="MSTGPTAATGSNRRLQQTQNQVDEVVDIMRVNVDKVLERDQKLS ELDDRADALQAGASQFETSAAKLKRKYWWKNCKMWAIGITVLVIFIIIIIVWVVSS" BASE COUNT 86 a 60 c 82 g 72 t ORIGIN 1 atgtctacag gtccaactgc tgccactggc agtaatcgaa gacttcagca gacacaaaat 61 caagtagatg aggtggtgga cataatgcga gttaacgtgg acaaggttct ggaaagagac 121 cagaagctct ctgagttaga cgaccgtgca gacgcactgc aggcaggcgc ttctcaattt 181 gaaacgagcg cagccaagtt gaagaggaaa tattggtgga agaattgcaa gatgtgggca 241 atcgggatta ctgttctggt tatcttcatc atcatcatca tcgtgtgggt tgtctcttca //