LOCUS       CR542129                 273 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834D1023D for
            gene PYY, peptide YY; complete cds, incl. stopcodon.
ACCESSION   CR542129
VERSION     CR542129.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 273)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  JOURNAL   Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834D1023D, ORFNo 4727
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1023D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            Contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is part of a collection of human full ORF clones
            jointly established and verified by the Harvard Institute of
            Proteomics (HIP) and RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC TCC ACC (ATG).
            The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
            The clone is validated by full sequence check.
            Compared to the reference sequence D13902
            we found AA exchange(s) at position (first base of changed
            triplet):
            214(thr->arg)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..273
                     /db_xref="H-InvDB:HIT000268998"
                     /organism="Homo sapiens"
                     /lab_host="DH5Alpha"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834D1023D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..273
                     /codon_start=1
                     /gene="PYY"
                     /db_xref="GOA:P10082"
                     /db_xref="H-InvDB:HIT000268998.12"
                     /db_xref="HGNC:HGNC:9748"
                     /db_xref="InterPro:IPR001955"
                     /db_xref="InterPro:IPR020392"
                     /db_xref="PDB:2DEZ"
                     /db_xref="PDB:2DF0"
                     /db_xref="PDB:2L60"
                     /db_xref="PDB:2NA5"
                     /db_xref="UniProtKB/Swiss-Prot:P10082"
                     /protein_id="CAG46926.1"
                     /translation="MVFVRRPWPALTTVLLALLVCLGALVDAYPIKPEAPGEDASPEE
                     LNRYYASLRHYLNLVTRQRYGKRDGPDRLLSKTFFPDGEDRPVRSR"
BASE COUNT           47 a          100 c           80 g           46 t
ORIGIN      
        1 atggtgttcg tgcgcaggcc gtggcccgcc ttgaccacag tgcttctggc cctgctcgtc
       61 tgcctagggg cgctggtcga cgcctacccc atcaaacccg aggctcccgg cgaagacgcc
      121 tcgccggagg agctgaaccg ctactacgcc tccctgcgcc actacctcaa cctggtcacc
      181 cggcagcggt atgggaaaag agacggcccg gacaggcttc tttccaaaac gttcttcccc
      241 gacggcgagg accgccccgt caggtcgcgg taa
//