LOCUS CR542078 1020 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E1136D for
gene NCF4, neutrophil cytosolic factor 4, 40kDa; complete cds,
incl. stopcodon.
ACCESSION CR542078
VERSION CR542078.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1020)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1020)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E1136D, ORFNo 4427
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E1136D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence AB025220
we found AA exchange(s) at position (first base of changed
triplet):
196(gln->arg)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1020
/db_xref="H-InvDB:HIT000268947"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E1136D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1020
/codon_start=1
/gene="NCF4"
/db_xref="GOA:Q6FGM9"
/db_xref="H-InvDB:HIT000268947.14"
/db_xref="InterPro:IPR000270"
/db_xref="InterPro:IPR000919"
/db_xref="InterPro:IPR001452"
/db_xref="InterPro:IPR001683"
/db_xref="InterPro:IPR034853"
/db_xref="InterPro:IPR034912"
/db_xref="InterPro:IPR035541"
/db_xref="InterPro:IPR036028"
/db_xref="InterPro:IPR036871"
/db_xref="UniProtKB/TrEMBL:Q6FGM9"
/protein_id="CAG46875.1"
/translation="MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIE
VKTKGGSKYLIYRRYRQFHALRSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIA
EMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSV
SPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGI
FPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRR
EFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRV
YNTMP"
BASE COUNT 236 a 302 c 289 g 193 t
ORIGIN
1 atggctgtgg cccagcagct gcgggccgag agtgactttg aacagcttcc ggatgatgtt
61 gccatctcgg ccaacattgc tgacatcgag gagaagagag gcttcaccag ccactttgtt
121 ttcgtcatcg aggtgaagac aaaaggagga tccaagtacc tcatctaccg ccgctaccgc
181 cagttccatg ctttgcggag caagctggag gagcgcttcg ggccagacag caagagcagt
241 gccctggcct gtaccctgcc cacactccca gccaaagtct acgtgggtgt gaaacaggag
301 atcgccgaga tgcggatacc tgccctcaac gcctacatga agagcctgct cagcctgccg
361 gtctgggtgc tgatggatga ggacgtccgg atcttctttt accagtcgcc ctatgactca
421 gagcaggtgc cccaggcact ccgccggctc cgcccgcgca cccggaaagt caagagcgtg
481 tccccacagg gcaacagcgt tgaccgcatg gcagctccga gagcagaggc tctatttgac
541 ttcactggaa acagcaaact ggagctgaat ttcaaagctg gagatgtgat cttcctcctc
601 agtcggatca acaaagactg gctggagggc actgtccggg gagccacggg catcttccct
661 ctctccttcg tgaagatcct caaagacttc cctgaggagg acgaccccac caactggctg
721 cgttgctact actacgaaga caccatcagc accatcaagg acatcgcggt ggaggaagat
781 ctcagcagca ctcccctatt gaaagacctg ctggagctca caaggcggga gttccagaga
841 gaggacatag ctctgaatta ccgggacgct gagggggatc tggttcggct gctgtcggat
901 gaggacgtag cgctcatggt gcggcaggct cgtggcctcc cctcccagaa gcgcctcttt
961 ccctggaagc tgcacatcac gcagaaggac aactacaggg tctacaacac gatgccatga
//