LOCUS CR542067 624 bp mRNA linear HUM 21-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0636D for
gene MRAS, muscle RAS oncogene homolog; complete cds, without
stopcodon.
ACCESSION CR542067
VERSION CR542067.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 624)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 624)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0636D, ORFNo 4401
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0636D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence AF493918
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..624
/db_xref="H-InvDB:HIT000268936"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0636D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>624
/codon_start=1
/gene="MRAS"
/db_xref="GOA:Q6FGP0"
/db_xref="H-InvDB:HIT000268936.12"
/db_xref="InterPro:IPR001806"
/db_xref="InterPro:IPR005225"
/db_xref="InterPro:IPR020849"
/db_xref="InterPro:IPR027417"
/db_xref="UniProtKB/TrEMBL:Q6FGP0"
/protein_id="CAG46864.1"
/translation="MATSAVPSDNLPTYKLVVVGDGGVGKSALTIQFFQKIFVPDYDP
TIEDSYLKHTEIDNQWAILDVLDTAGQEEFSAMREQYMRTGDGFLIVYSVTDKASFEH
VDRFHQLILRVKDRESFPMILVANKVDLMHLRKITREQGKEMATKHNIPYIETSAKDP
PLNVDKAFHDLVRVIRQQIPEKSQKKKKKTKWRGDRATGTHKLQCVIL"
BASE COUNT 179 a 167 c 162 g 116 t
ORIGIN
1 atggcaacca gcgccgtccc cagtgacaac ctccccacat acaagctggt ggtggtgggg
61 gatgggggtg tgggcaaaag tgccctcacc atccagtttt tccagaagat ctttgtgcct
121 gactatgacc ccaccattga agactcctac ctgaaacata cggagattga caatcaatgg
181 gccatcttgg acgttctgga cacagctggg caggaggaat tcagcgccat gcgggagcaa
241 tacatgcgca cgggggatgg cttcctcatc gtctactccg tcactgacaa ggccagcttt
301 gagcacgtgg accgcttcca ccagcttatc ctgcgcgtca aagacaggga gtcattcccg
361 atgatcctcg tggccaacaa ggtcgatttg atgcacttga ggaagatcac cagggagcaa
421 ggaaaagaaa tggcgaccaa acacaatatt ccgtacatag aaaccagtgc caaggaccca
481 cctctcaatg tcgacaaagc cttccatgac ctcgttagag taattaggca acagattccg
541 gaaaaaagcc agaagaagaa gaagaaaacc aaatggcggg gagaccgggc cacaggcacc
601 cacaaactgc aatgtgtgat cttg
//