LOCUS CR542055 1014 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0836D for
gene PHYH, phytanoyl-CoA hydroxylase (Refsum disease); complete
cds, without stopcodon.
ACCESSION CR542055
VERSION CR542055.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1014)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1014)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D0836D, ORFNo 4348
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0836D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131223.01L
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_006214 (GI:17999532)
we found AA exchange(s) at position (first base of changed
triplet):
631(gly->asp)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1014
/db_xref="H-InvDB:HIT000268924"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D0836D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1014
/codon_start=1
/gene="PHYH"
/db_xref="H-InvDB:HIT000268924.13"
/db_xref="InterPro:IPR008775"
/db_xref="UniProtKB/TrEMBL:Q6FGQ2"
/protein_id="CAG46852.1"
/translation="MEQLRAAARLQIVLGHLGRPSAGAVVAHPTSGTISSASFHPQQF
QYTLDNNVLTLEQRKFYEENGFLVIKNLVPDADIQRFRNEFEKICRKEVKPLGLTVMR
DVTISKSEYAPSEKMITKVQDFQEDKELFRYCTLPEILKYVECFTGPNIMAMHTMLIN
KPPDSGKKTSRHPLHQDLHYFPFRPSDLIVCAWTAMEHISRNNGCLVVLPDTHKGSLK
PHDYPKWEGGVNKMFHGIQDYEENKARVHLVMEKGDTVFFHPLLIHGSGQNKTQGFRK
AISCHFASADCHYIDVKGTSQENIEKEVVGIAHKFFGAENSVNLKDIWMFRARLVKGE
RTNL"
BASE COUNT 284 a 250 c 245 g 235 t
ORIGIN
1 atggagcagc ttcgcgccgc cgcccgtctg cagattgttc tgggccacct cggccgcccc
61 tcggccgggg ctgtcgtagc tcatcccact tcagggacta tttcctctgc cagtttccat
121 cctcaacaat tccagtatac tctggataat aatgttctaa ccctggaaca gagaaaattt
181 tatgaagaaa atgggtttct agtaatcaaa aatcttgtac ctgatgccga tattcaacgc
241 tttcggaatg agtttgaaaa aatctgcaga aaggaggtga aaccattagg attaacagta
301 atgagagatg tgaccatttc gaaatccgaa tatgctccaa gtgagaagat gatcacgaag
361 gtccaggatt tccaggaaga taaggagctc ttcagatact gcactctccc cgagattctg
421 aaatatgtgg agtgcttcac tggacctaat attatggcca tgcacacaat gttgataaac
481 aaacctccag attctggcaa gaagacgtcc cgtcaccccc tgcaccagga cctgcactat
541 ttccccttca ggcccagcga tctcatcgtt tgcgcctgga cggcgatgga gcacatcagc
601 cggaacaacg gctgtctggt tgtgctccca gacacacaca agggctccct gaagccccac
661 gattacccca agtgggaggg gggagttaac aaaatgttcc acgggatcca ggactacgag
721 gaaaacaagg cccgggtgca cctggtgatg gagaagggcg acactgtttt cttccatcct
781 ttgctcatcc acggatctgg tcagaataaa acccagggat tccggaaggc aatttcctgc
841 catttcgcca gtgccgattg ccactacatt gacgtgaagg gcaccagtca agaaaacatc
901 gagaaggaag ttgtaggaat agcacataaa ttctttggag ctgaaaatag cgtgaacttg
961 aaggatattt ggatgtttcg agctcgactt gtgaaaggag aaagaaccaa tctt
//