LOCUS       CR542037                 678 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834B0136D for
            gene BCAS2, breast carcinoma amplified sequence 2; complete cds,
            incl. stopcodon.
ACCESSION   CR542037
VERSION     CR542037.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 678)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  JOURNAL   Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834B0136D, ORFNo 4310
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0136D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            Contact RZPD (customer.service@rzpd.de) for further information.
            Clone name at Harvard Institute of Proteomics
            (www.hip.harvard.edu):
            FLH131203.01X
            
            This CDS clone is part of a collection of human full ORF clones
            jointly established and verified by the Harvard Institute of
            Proteomics (HIP) and RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC TCC ACC (ATG).
            The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
            The clone is validated by full sequence check.
            Compared to the reference sequence NM_005872 (GI:5031652)
            we found AA exchange(s) at position (first base of changed
            triplet):
            70(glu->asp) 265(leu->val)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..678
                     /db_xref="H-InvDB:HIT000268906"
                     /organism="Homo sapiens"
                     /lab_host="DH5Alpha"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834B0136D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..678
                     /codon_start=1
                     /gene="BCAS2"
                     /db_xref="GOA:O75934"
                     /db_xref="H-InvDB:HIT000268906.13"
                     /db_xref="HGNC:HGNC:975"
                     /db_xref="InterPro:IPR008409"
                     /db_xref="PDB:5MQF"
                     /db_xref="PDB:5XJC"
                     /db_xref="PDB:5YZG"
                     /db_xref="PDB:5Z56"
                     /db_xref="PDB:5Z57"
                     /db_xref="PDB:6FF7"
                     /db_xref="PDB:6ICZ"
                     /db_xref="PDB:6ID0"
                     /db_xref="PDB:6ID1"
                     /db_xref="PDB:6QDV"
                     /db_xref="UniProtKB/Swiss-Prot:O75934"
                     /protein_id="CAG46834.1"
                     /translation="MAGTGLVAGEVVVDALPYFDQGYDAPGVREAAAALVEEETRRYR
                     PTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYEVPAPSSGQKNDITA
                     WQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHI
                     QDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANK
                     ENIRQDF"
BASE COUNT          228 a          125 c          167 g          158 t
ORIGIN      
        1 atggcgggca caggtttggt ggctggagag gttgtggtgg atgcgctgcc gtattttgat
       61 caaggttatg atgcccctgg tgtgcgggaa gcggctgcag cgctggtgga ggaggaaact
      121 cgcagatacc gacctactaa gaactacctg agctacctga cagccccgga ttattctgcc
      181 tttgaaactg acataatgag aaatgaattt gaaagactgg ctgctcgaca accaattgaa
      241 ttgctcagta tgaaacgata tgaggttcca gccccctcct ctggtcaaaa aaatgacatt
      301 actgcatggc aagaatgtgt aaacaattct atggcccagt tagagcatca agcagttaga
      361 attgagaatc tggaactaat gtcacagcat ggatgtaatg cctggaaagt atacaatgaa
      421 aatctagttc atatgattga acacgcacag aaggaacttc agaagttaag aaaacatatt
      481 caagatttaa actggcagag aaagaacatg caactcacag ctggatctaa attgagagaa
      541 atggagtcaa attgggtatc cctggtcagt aagaattatg agattgaacg gactattgtt
      601 cagctagaaa atgaaatcta tcaaattaag cagcaacatg gagaggcaaa caaagaaaac
      661 atccggcaag acttctga
//