LOCUS CR542037 678 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0136D for gene BCAS2, breast carcinoma amplified sequence 2; complete cds, incl. stopcodon. ACCESSION CR542037 VERSION CR542037.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 678) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 678) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834B0136D, ORFNo 4310 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0136D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. Clone name at Harvard Institute of Proteomics (www.hip.harvard.edu): FLH131203.01X This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_005872 (GI:5031652) we found AA exchange(s) at position (first base of changed triplet): 70(glu->asp) 265(leu->val) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..678 /db_xref="H-InvDB:HIT000268906" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834B0136D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..678 /codon_start=1 /gene="BCAS2" /db_xref="GOA:O75934" /db_xref="H-InvDB:HIT000268906.13" /db_xref="HGNC:HGNC:975" /db_xref="InterPro:IPR008409" /db_xref="PDB:5MQF" /db_xref="PDB:5XJC" /db_xref="PDB:5YZG" /db_xref="PDB:5Z56" /db_xref="PDB:5Z57" /db_xref="PDB:6FF7" /db_xref="PDB:6ICZ" /db_xref="PDB:6ID0" /db_xref="PDB:6ID1" /db_xref="PDB:6QDV" /db_xref="UniProtKB/Swiss-Prot:O75934" /protein_id="CAG46834.1" /translation="MAGTGLVAGEVVVDALPYFDQGYDAPGVREAAAALVEEETRRYR PTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYEVPAPSSGQKNDITA WQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHI QDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANK ENIRQDF" BASE COUNT 228 a 125 c 167 g 158 t ORIGIN 1 atggcgggca caggtttggt ggctggagag gttgtggtgg atgcgctgcc gtattttgat 61 caaggttatg atgcccctgg tgtgcgggaa gcggctgcag cgctggtgga ggaggaaact 121 cgcagatacc gacctactaa gaactacctg agctacctga cagccccgga ttattctgcc 181 tttgaaactg acataatgag aaatgaattt gaaagactgg ctgctcgaca accaattgaa 241 ttgctcagta tgaaacgata tgaggttcca gccccctcct ctggtcaaaa aaatgacatt 301 actgcatggc aagaatgtgt aaacaattct atggcccagt tagagcatca agcagttaga 361 attgagaatc tggaactaat gtcacagcat ggatgtaatg cctggaaagt atacaatgaa 421 aatctagttc atatgattga acacgcacag aaggaacttc agaagttaag aaaacatatt 481 caagatttaa actggcagag aaagaacatg caactcacag ctggatctaa attgagagaa 541 atggagtcaa attgggtatc cctggtcagt aagaattatg agattgaacg gactattgtt 601 cagctagaaa atgaaatcta tcaaattaag cagcaacatg gagaggcaaa caaagaaaac 661 atccggcaag acttctga //