LOCUS CR542008 594 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F0235D for
gene YKT6, SNARE protein Ykt6; complete cds, without stopcodon.
ACCESSION CR542008
VERSION CR542008.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 594)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 594)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F0235D, ORFNo 4251
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F0235D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131248.01L
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_006555 (GI:34304384)
we found AA exchange(s) at position (first base of changed
triplet):
187(asp->asn)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..594
/db_xref="H-InvDB:HIT000268877"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F0235D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>594
/codon_start=1
/gene="YKT6"
/db_xref="GOA:O15498"
/db_xref="H-InvDB:HIT000268877.11"
/db_xref="HGNC:HGNC:16959"
/db_xref="InterPro:IPR001388"
/db_xref="InterPro:IPR010908"
/db_xref="InterPro:IPR011012"
/db_xref="UniProtKB/Swiss-Prot:O15498"
/protein_id="CAG46805.1"
/translation="MKLYSLSVLYKGEAKVVLLKAAYDVSSFSFFQRSSVQEFMTFTS
QLIVERSSKGTRASVKEQNYLCHVYVRNDSLAGVVIADNEYPSRVAFTLLEKVLDEFS
KQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDETKIILHNTMESL
LERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM"
BASE COUNT 165 a 152 c 143 g 134 t
ORIGIN
1 atgaagctgt acagcctcag cgtcctctac aaaggcgagg ccaaggtggt gctgctcaaa
61 gccgcatacg atgtgtcttc cttcagcttt ttccagagat ccagcgttca ggaattcatg
121 accttcacga gtcaactgat tgtggagcgc tcatcgaaag gcactagagc ttctgtcaaa
181 gaacaaaact atctgtgcca cgtctacgtc cggaatgata gtcttgcagg tgtggtcatt
241 gctgacaatg aatacccatc ccgggtggcc tttaccttgc tggagaaggt actagatgaa
301 ttctccaagc aagtcgacag gatagactgg ccagtaggat cccctgctac aatccattac
361 ccagccctgg atggtcacct cagtagatac cagaacccac gagaagctga tcccatgact
421 aaagtgcagg ccgaactaga tgagaccaaa atcattctgc acaacaccat ggagtctctg
481 ttagagcgag gtgagaagct agatgacttg gtgtccaaat ccgaggtgct gggaacgcag
541 tctaaagcct tctataaaac tgcccggaaa caaaactcat gctgtgccat catg
//