LOCUS CR541998 576 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B1135D for gene PMVK, phosphomevalonate kinase; complete cds, without stopcodon. ACCESSION CR541998 VERSION CR541998.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 576) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 576) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834B1135D, ORFNo 4239 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B1135D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. Clone name at Harvard Institute of Proteomics (www.hip.harvard.edu): FLH131282.01L This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_006556 (GI:20127505) we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..576 /db_xref="H-InvDB:HIT000268867" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834B1135D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>576 /codon_start=1 /gene="PMVK" /db_xref="GOA:Q6FGV9" /db_xref="H-InvDB:HIT000268867.13" /db_xref="InterPro:IPR005919" /db_xref="InterPro:IPR027417" /db_xref="UniProtKB/TrEMBL:Q6FGV9" /protein_id="CAG46795.1" /translation="MAPLGGAPRLVLLFSGKRKSGKDFVTEALQSRLGADVCAVLRLS GPLKEQYAQEHGLNFQRLLDTSTYKEAFRKDMIRWGEEKRQADPGFFCRKIVEGISQP IWLVSDTRRVSDIQWFREAYGAVTQTVRVVALEQSRQQRGWVFTPGVDDAESECGLDN FGDFDWVIENHGVEQRLEEQLENLIEFIRSRL" BASE COUNT 122 a 144 c 197 g 113 t ORIGIN 1 atggccccgc tgggaggcgc cccgcggctg gtactgctgt tcagcggcaa gaggaaatcc 61 gggaaggact tcgtgaccga ggcgctgcag agcagacttg gagctgatgt ctgtgctgtc 121 ctccggctct ctggtccact caaggaacag tatgctcagg agcatggctt gaacttccag 181 agactcctgg acaccagcac ctacaaggag gcctttcgga aggacatgat ccgctgggga 241 gaggagaaac gccaggctga cccaggcttc ttttgcagga agattgtgga gggcatctcc 301 cagcccatct ggctggtgag tgacacacgg agagtgtctg acatccagtg gtttcgggag 361 gcctatgggg ccgtgacgca gacggtccgc gttgtagcgt tggagcagag ccgacagcag 421 cggggctggg tgttcacgcc aggggtggac gatgctgagt cagaatgtgg cctggacaac 481 ttcggggact ttgactgggt catcgagaac catggagttg aacagcgcct ggaggagcag 541 ttggagaacc tgatagaatt tatccgctcc agactt //