LOCUS CR541993 537 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0835D for
gene IL10, interleukin 10; complete cds, incl. stopcodon.
ACCESSION CR541993
VERSION CR541993.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 537)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 537)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0835D, ORFNo 4221
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0835D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131273.01X
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_000572 (GI:24430216)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..537
/db_xref="H-InvDB:HIT000268862"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0835D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..537
/codon_start=1
/gene="IL10"
/db_xref="GOA:Q6FGW4"
/db_xref="H-InvDB:HIT000268862.12"
/db_xref="InterPro:IPR000098"
/db_xref="InterPro:IPR009079"
/db_xref="InterPro:IPR020423"
/db_xref="InterPro:IPR020443"
/db_xref="UniProtKB/TrEMBL:Q6FGW4"
/protein_id="CAG46790.1"
/translation="MHSSALLCCLVLLTGVRASPGQGTQSENSCTHFPGNLPNMLRDL
RDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQD
PDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSE
FDIFINYIEAYMTMKIRN"
BASE COUNT 146 a 136 c 140 g 115 t
ORIGIN
1 atgcacagct cagcactgct ctgttgcctg gtcctcctga ctggggtgag ggccagccca
61 ggccagggca cccagtctga gaacagctgc acccacttcc caggcaacct gcctaacatg
121 cttcgagatc tccgagatgc cttcagcaga gtgaagactt tctttcaaat gaaggatcag
181 ctggacaact tgttgttaaa ggagtccttg ctggaggact ttaagggtta cctgggttgc
241 caagccttgt ctgagatgat ccagttttac ctggaggagg tgatgcccca agctgagaac
301 caagacccag acatcaaggc gcatgtgaac tccctggggg agaacctgaa gaccctcagg
361 ctgaggctac ggcgctgtca tcgatttctt ccctgtgaaa acaagagcaa ggccgtggag
421 caggtgaaga atgcctttaa taagctccaa gagaaaggca tctacaaagc catgagtgag
481 tttgacatct tcatcaacta catagaagcc tacatgacaa tgaagatacg aaactga
//