LOCUS CR541990 450 bp mRNA linear HUM 17-APR-2005 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0735D for gene CALM2, calmodulin 2 (phosphorylase kinase, delta); complete cds, incl. stopcodon. ACCESSION CR541990 VERSION CR541990.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 450) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 450) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834B0735D, ORFNo 4216 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0735D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. Clone name at Harvard Institute of Proteomics (www.hip.harvard.edu): FLH131267.01X This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_001743 (GI:20428653) we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..450 /db_xref="H-InvDB:HIT000268859" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834B0735D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..450 /codon_start=1 /gene="CALM2" /db_xref="GOA:P0DP24" /db_xref="H-InvDB:HIT000268859.14" /db_xref="HGNC:HGNC:1445" /db_xref="InterPro:IPR002048" /db_xref="InterPro:IPR011992" /db_xref="InterPro:IPR018247" /db_xref="InterPro:IPR039030" /db_xref="PDB:3O77" /db_xref="PDB:3O78" /db_xref="PDB:5COC" /db_xref="PDB:5J03" /db_xref="PDB:5NIN" /db_xref="PDB:5VMS" /db_xref="PDB:5WSU" /db_xref="PDB:5WSV" /db_xref="UniProtKB/Swiss-Prot:P0DP24" /protein_id="CAG46787.1" /translation="MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNP TEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIS AAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK" BASE COUNT 167 a 61 c 115 g 107 t ORIGIN 1 atggctgacc aactgactga agagcagatt gcagaattca aagaagcttt ttcactattt 61 gacaaagatg gtgatggaac tataacaaca aaggaattgg gaactgtaat gagatctctt 121 gggcagaatc ccacagaagc agagttacag gacatgatta atgaagtaga tgctgatggt 181 aatggcacaa ttgacttccc tgaatttctg acaatgatgg caagaaaaat gaaagacaca 241 gacagtgaag aagaaattag agaagcattc cgtgtgtttg ataaggatgg caatggctat 301 attagtgctg cagaacttcg ccatgtgatg acaaaccttg gagagaagtt aacagatgaa 361 gaagttgatg aaatgatcag ggaagcagat attgatggtg atggtcaagt aaactatgaa 421 gagtttgtac aaatgatgac agcaaagtga //