LOCUS CR541972 399 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E1234D for gene LGALS2, lectin, galactoside-binding, soluble, 2 (galectin 2); complete cds, incl. stopcodon. ACCESSION CR541972 VERSION CR541972.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 399) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 399) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834E1234D, ORFNo 4182 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E1234D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_006498 (GI:5729902) we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..399 /db_xref="H-InvDB:HIT000268842" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834E1234D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..399 /codon_start=1 /gene="LGALS2" /db_xref="GOA:P05162" /db_xref="H-InvDB:HIT000268842.12" /db_xref="HGNC:HGNC:6562" /db_xref="InterPro:IPR001079" /db_xref="InterPro:IPR013320" /db_xref="PDB:1HLC" /db_xref="PDB:5DG1" /db_xref="PDB:5DG2" /db_xref="PDB:5EWS" /db_xref="UniProtKB/Swiss-Prot:P05162" /protein_id="CAG46770.1" /translation="MTGELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNL HFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTFESDKFKVKLPDGHE LTFPNRLGHSHLSYLSVRGGFNMSSFKLKE" BASE COUNT 111 a 97 c 111 g 80 t ORIGIN 1 atgacggggg aacttgaggt taagaacatg gacatgaagc cggggtcaac cctgaagatc 61 acaggcagca tcgccgatgg cactgatggc tttgtaatta atctgggcca ggggacagac 121 aagctgaacc tgcatttcaa ccctcgcttc agcgaatcca ccattgtctg caactcattg 181 gacggcagca actgggggca agaacaacgg gaagatcacc tgtgcttcag cccagggtca 241 gaggtcaagt tcacagtgac ctttgagagt gacaaattca aggtgaagct gccagatggg 301 cacgagctga cttttcccaa caggctgggt cacagccacc tgagctacct gagcgtaagg 361 ggcgggttca acatgtcctc tttcaagtta aaagaataa //