LOCUS CR541963 1365 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0734D for
gene EPHX1, epoxide hydrolase 1, microsomal (xenobiotic); complete
cds, without stopcodon.
ACCESSION CR541963
VERSION CR541963.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1365)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1365)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0734D, ORFNo 4155
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0734D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_000120 (GI:4557560)
we found AA exchange(s) at position (first base of changed
triplet):
991(thr->ile)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1365
/db_xref="H-InvDB:HIT000268833"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0734D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1365
/codon_start=1
/gene="EPHX1"
/db_xref="GOA:Q6FGZ3"
/db_xref="H-InvDB:HIT000268833.13"
/db_xref="InterPro:IPR000639"
/db_xref="InterPro:IPR010497"
/db_xref="InterPro:IPR016292"
/db_xref="InterPro:IPR029058"
/db_xref="UniProtKB/TrEMBL:Q6FGZ3"
/protein_id="CAG46761.1"
/translation="MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRSAARE
DDSIRPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFD
WKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGHTPKPLLMVHGWPGSFYEFYKI
IPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQ
EFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGQRFGRFLGLTE
RDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSIWTN
TEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKV
YVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLS
VLERQ"
BASE COUNT 306 a 385 c 362 g 312 t
ORIGIN
1 atgtggctag aaatcctcct cacttcagtg ctgggctttg ccatctactg gttcatctcc
61 cgggacaaag aggaaacttt gccacttgaa gatgggtggt gggggccagg cacgaggtcc
121 gcagccaggg aggacgacag catccgccct ttcaaggtgg aaacgtcaga tgaggagatc
181 cacgacttac accagaggat cgataagttc cgtttcaccc cacctttgga ggacagctgc
241 ttccactatg gcttcaactc caactacctg aagaaagtca tctcctactg gcggaatgaa
301 tttgactgga agaagcaggt ggagattctc aacagatacc ctcacttcaa gactaagatt
361 gaagggctgg acatccactt catccacgtg aagccccccc agctgcccgc aggccatacc
421 ccgaagccct tgctgatggt gcacggctgg cccggctctt tctacgagtt ttataagatc
481 atcccactcc tgactgaccc caagaaccat ggcctgagcg atgagcacgt ttttgaagtc
541 atctgccctt ccatccctgg ctatggcttc tcagaggcat cctccaagaa ggggttcaac
601 tcggtggcca ccgccaggat cttttacaag ctgatgctgc ggctgggctt ccaggaattc
661 tacattcaag gaggggactg ggggtccctg atctgcacta atatggccca gctggtgccc
721 agccacgtga aaggcctgca cttgaacatg gctttggttt taagcaactt ctctaccctg
781 accctcctcc tgggacagcg tttcgggagg tttcttggcc tcactgagag ggatgtggag
841 ctgctgtacc ccgtcaagga gaaggtattc tacagcctga tgagggagag cggctacatg
901 cacatccagt gcaccaagcc tgacaccgta ggctctgctc tgaatgactc tcctgtgggt
961 ctggctgcct atattctaga gaagttttcc atctggacca atacggaatt ccgatacctg
1021 gaggatggag gcctggaaag gaagttctcc ctggacgacc tgctgaccaa cgtcatgctc
1081 tactggacaa caggcaccat catctcctcc cagcgcttct acaaggagaa cctgggacag
1141 ggctggatga cccagaagca tgagcggatg aaggtctatg tgcccactgg cttctctgcc
1201 ttcccttttg agctattgca cacgcctgaa aagtgggtga ggttcaagta cccaaagctc
1261 atctcctatt cctacatggt tcgtgggggc cactttgcgg cctttgagga gccggagctg
1321 ctcgcccagg acatccgcaa gttcctgtcg gtgctggagc ggcaa
//