LOCUS CR541953 480 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0234D for gene PMP22, peripheral myelin protein 22; complete cds, without stopcodon. ACCESSION CR541953 VERSION CR541953.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 480) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 480) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834E0234D, ORFNo 4048 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0234D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_000304 (GI:24430161) we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..480 /db_xref="H-InvDB:HIT000268823" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834E0234D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>480 /codon_start=1 /gene="PMP22" /db_xref="GOA:Q6FH25" /db_xref="H-InvDB:HIT000268823.11" /db_xref="InterPro:IPR003936" /db_xref="InterPro:IPR004031" /db_xref="InterPro:IPR004032" /db_xref="UniProtKB/TrEMBL:Q6FH25" /protein_id="CAG46751.1" /translation="MLLLLLSIIVLHVAVLVLLFVSTIVSQWIVGNGHATDLWQNCST SSSGNVHHCFSSSPNEWLQSVQATMILSIIFSILSLFLFFCQLFTLTKGGRFYITGIF QILAGLCVMSAAAIYTVRHPEWHLNSDYSYGFAYILAWVAFPLALLSGVIYVILRKRE " BASE COUNT 86 a 148 c 113 g 133 t ORIGIN 1 atgctcctcc tgttgctgag tatcatcgtc ctccacgtcg cggtgctggt gctgctgttc 61 gtctccacga tcgtcagcca atggatcgtg ggcaatggac acgcaactga tctctggcag 121 aactgtagca cctcttcctc aggaaatgtc caccactgtt tctcatcatc accaaacgaa 181 tggctgcagt ctgtccaggc caccatgatc ctgtcgatca tcttcagcat tctgtctctg 241 ttcctgttct tctgccaact cttcaccctc accaaggggg gcaggtttta catcactgga 301 atcttccaaa ttcttgctgg tctgtgcgtg atgagtgctg cggccatcta cacggtgagg 361 cacccggagt ggcatctcaa ctcggattac tcctacggtt tcgcctacat cctggcctgg 421 gtggccttcc ccctggccct tctcagcggt gtcatctatg tgatcttgcg gaaacgcgaa //