LOCUS CR541948 1125 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B1234D for
gene SFTPD, surfactant, pulmonary-associated protein D; complete
cds, without stopcodon.
ACCESSION CR541948
VERSION CR541948.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1125)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1125)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B1234D, ORFNo 4039
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B1234D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131307.01L
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_003019 (GI:42476329)
we found AA exchange(s) at position (first base of changed
triplet):
1105(arg->ser)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1125
/db_xref="H-InvDB:HIT000268818"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B1234D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1125
/codon_start=1
/gene="SFTPD"
/db_xref="GOA:P35247"
/db_xref="H-InvDB:HIT000268818.13"
/db_xref="HGNC:HGNC:10803"
/db_xref="InterPro:IPR001304"
/db_xref="InterPro:IPR008160"
/db_xref="InterPro:IPR015097"
/db_xref="InterPro:IPR016186"
/db_xref="InterPro:IPR016187"
/db_xref="InterPro:IPR018378"
/db_xref="InterPro:IPR033990"
/db_xref="PDB:1B08"
/db_xref="PDB:1M7L"
/db_xref="PDB:1PW9"
/db_xref="PDB:1PWB"
/db_xref="PDB:2GGU"
/db_xref="PDB:2GGX"
/db_xref="PDB:2ORJ"
/db_xref="PDB:2ORK"
/db_xref="PDB:2OS9"
/db_xref="PDB:2RIA"
/db_xref="PDB:2RIB"
/db_xref="PDB:2RIC"
/db_xref="PDB:2RID"
/db_xref="PDB:2RIE"
/db_xref="PDB:3DBZ"
/db_xref="PDB:3G81"
/db_xref="PDB:3G83"
/db_xref="PDB:3G84"
/db_xref="PDB:3IKN"
/db_xref="PDB:3IKP"
/db_xref="PDB:3IKQ"
/db_xref="PDB:3IKR"
/db_xref="PDB:4E52"
/db_xref="PDB:4M17"
/db_xref="PDB:4M18"
/db_xref="PDB:5OXR"
/db_xref="PDB:5OXS"
/db_xref="UniProtKB/Swiss-Prot:P35247"
/protein_id="CAG46746.1"
/translation="MLLFLLSALVLLTQPLGYLEAEMKTYSHRTTPSACTLVMCSSVE
SGLPGRDGRDGREGPRGEKGDPGLPGAAGQAGMPGQAGPVGPKGDNGSVGEPGPKGDT
GPSGPPGPPGVPGPAGREGPLGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSAGARG
LAGPKGERGVPGERGVPGNAGAAGSAGAMGPQGSPGARGPPGLKGDKGIPGDKGAKGE
SGLPDVASLRQQVEALQGQVQHLQAAFSQYKKVELFPNGQSVGEKIFKTAGFVKPFTE
AQLLCTQAGGQLASPRSAAENAALQQLVVAKNEAAFLSMTDSKTEGKFTYPTGESLVY
SNWAPGEPNDDGGSEDCVEIFTNGKWNDRACGEKSLVVCEF"
BASE COUNT 265 a 287 c 379 g 194 t
ORIGIN
1 atgctgctct tcctcctctc tgcactggtc ctgctcacac agcccctggg ctacctggaa
61 gcagaaatga agacctactc ccacagaaca acgcccagtg cttgcaccct ggtcatgtgt
121 agctcagtgg agagtggcct gcctggtcgc gatggacggg atgggagaga gggccctcgg
181 ggcgagaagg gggacccagg tttgccagga gctgcagggc aagcagggat gcctggacaa
241 gctggcccag ttgggcccaa aggggacaat ggctctgttg gagaacctgg accaaaggga
301 gacactgggc caagtggacc tccaggacct cccggtgtgc ctggtccagc tggaagagaa
361 ggtcccctgg ggaagcaggg gaacatagga cctcagggca agccaggccc aaagggagaa
421 gctgggccca aaggagaagt aggtgcccca ggcatgcagg gctcggcagg ggcaagaggc
481 ctcgcaggcc ctaagggaga gcgaggtgtc cctggtgagc gtggagtccc tggaaacgca
541 ggggcagcag ggtctgctgg agccatgggt ccccagggaa gtccaggtgc caggggaccc
601 ccgggattga agggggacaa aggcattcct ggagacaaag gagcaaaggg agaaagtggg
661 cttccagatg ttgcttctct gaggcagcag gttgaggcct tacagggaca agtacagcac
721 ctccaggctg ctttctctca gtataagaaa gttgagctct tcccaaatgg ccaaagtgtc
781 ggggagaaga ttttcaagac agcaggcttt gtaaaaccat ttacggaggc acagctgctg
841 tgcacacagg ctggtggaca gttggcctct ccacgctctg ccgctgagaa tgccgccttg
901 caacagctgg tcgtagctaa gaacgaggct gctttcctga gcatgactga ttccaagaca
961 gagggcaagt tcacctaccc cacaggagag tccctggtct attccaactg ggccccaggg
1021 gagcccaacg atgatggcgg gtcagaggac tgtgtggaga tcttcaccaa tggcaagtgg
1081 aatgacaggg cttgtggaga aaagagtctt gtggtctgcg agttc
//