LOCUS CR541946 1080 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D1134D for
gene DCN, decorin; complete cds, incl. stopcodon.
ACCESSION CR541946
VERSION CR541946.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1080)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1080)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D1134D, ORFNo 4033
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1134D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_133503 (GI:19743845)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1080
/db_xref="H-InvDB:HIT000268816"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D1134D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1080
/codon_start=1
/gene="DCN"
/db_xref="GOA:Q6FH10"
/db_xref="H-InvDB:HIT000268816.13"
/db_xref="InterPro:IPR000372"
/db_xref="InterPro:IPR001611"
/db_xref="InterPro:IPR003591"
/db_xref="InterPro:IPR016352"
/db_xref="InterPro:IPR028549"
/db_xref="InterPro:IPR032675"
/db_xref="UniProtKB/TrEMBL:Q6FH10"
/protein_id="CAG46744.1"
/translation="MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDR
DFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFK
NLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHEN
EITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQG
LPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHL
DNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPV
QYWEIQPSTFRCVYVRSAIQLGNYK"
BASE COUNT 306 a 267 c 236 g 271 t
ORIGIN
1 atgaaggcca ctatcatcct ccttctgctt gcacaagttt cctgggctgg accgtttcaa
61 cagagaggct tatttgactt tatgctagaa gatgaggctt ctgggatagg cccagaagtt
121 cctgatgacc gcgacttcga gccctcccta ggcccagtgt gccccttccg ctgtcaatgc
181 catcttcgag tggtccagtg ttctgatttg ggtctggaca aagtgccaaa ggatcttccc
241 cctgacacaa ctctgctaga cctgcaaaac aacaaaataa ccgaaatcaa agatggagac
301 tttaagaacc tgaagaacct tcacgcattg attcttgtca acaataaaat tagcaaagtt
361 agtcctggag catttacacc tttggtgaag ttggaacgac tttatctgtc caagaatcag
421 ctgaaggaat tgccagaaaa aatgcccaaa actcttcagg agctgcgtgc ccatgagaat
481 gagatcacca aagtgcgaaa agttactttc aatggactga accagatgat tgtcatagaa
541 ctgggcacca atccgctgaa gagctcagga attgaaaatg gggctttcca gggaatgaag
601 aagctctcct acatccgcat tgctgatacc aatatcacca gcattcctca aggtcttcct
661 ccttccctta cggaattaca tcttgatggc aacaaaatca gcagagttga tgcagctagc
721 ctgaaaggac tgaataattt ggctaagttg ggattgagtt tcaacagcat ctctgctgtt
781 gacaatggct ctctggccaa cacgcctcat ctgagggagc ttcacttgga caacaacaag
841 cttaccagag tacctggtgg gctggcagag cataagtaca tccaggttgt ctaccttcat
901 aacaacaata tctctgtagt tggatcaagt gacttctgcc cacctggaca caacaccaaa
961 aaggcttctt attcgggtgt gagtcttttc agcaacccgg tccagtactg ggagatacag
1021 ccatccacct tcagatgtgt ctacgtgcgc tctgccattc aactcggaaa ctataagtaa
//