LOCUS       CR541908                 939 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834E0433D for
            gene ASPA, aspartoacylase (aminoacylase 2, Canavan disease);
            complete cds, without stopcodon.
ACCESSION   CR541908
VERSION     CR541908.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 939)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  JOURNAL   Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834E0433D, ORFNo 3927
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0433D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            Contact RZPD (customer.service@rzpd.de) for further information.
            Clone name at Harvard Institute of Proteomics
            (www.hip.harvard.edu):
            FLH130849.01L
            
            This CDS clone is part of a collection of human full ORF clones
            jointly established and verified by the Harvard Institute of
            Proteomics (HIP) and RZPD.
            This CDS has been cloned without stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC TCC ACC (ATG).
            The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
            The clone is validated by full sequence check.
            Compared to the reference sequence NM_000049 (GI:4557334)
            we did not find any amino acid exchanges.
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..939
                     /db_xref="H-InvDB:HIT000268778"
                     /organism="Homo sapiens"
                     /lab_host="DH5Alpha"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834E0433D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..>939
                     /codon_start=1
                     /gene="ASPA"
                     /db_xref="GOA:Q6FH48"
                     /db_xref="H-InvDB:HIT000268778.13"
                     /db_xref="InterPro:IPR007036"
                     /db_xref="InterPro:IPR016708"
                     /db_xref="UniProtKB/TrEMBL:Q6FH48"
                     /protein_id="CAG46706.1"
                     /translation="MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRT
                     GLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGP
                     KDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHP
                     SLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPC
                     AIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDC
                     TVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH"
BASE COUNT          290 a          188 c          196 g          265 t
ORIGIN      
        1 atgacttctt gtcacattgc tgaagaacat atacaaaagg ttgctatctt tggaggaacc
       61 catgggaatg agctaaccgg agtatttctg gttaagcatt ggctagagaa tggcgctgag
      121 attcagagaa cagggctgga ggtaaaacca tttattacta accccagagc agtgaagaag
      181 tgtaccagat atattgactg tgacctgaat cgcatttttg accttgaaaa tcttggcaaa
      241 aaaatgtcag aagatttgcc atatgaagtg agaagggctc aagaaataaa tcatttattt
      301 ggtccaaaag acagtgaaga ttcctatgac attatttttg accttcacaa caccacctct
      361 aacatggggt gcactcttat tcttgaggat tccaggaata actttttaat tcagatgttt
      421 cattacatta agacttctct ggctccacta ccctgctacg tttatctgat tgagcatcct
      481 tccctcaaat atgcgaccac tcgttccata gccaagtatc ctgtgggtat agaagttggt
      541 cctcagcctc aaggggttct gagagctgat atcttggatc aaatgagaaa aatgattaaa
      601 catgctcttg attttataca tcatttcaat gaaggaaaag aatttcctcc ctgcgccatt
      661 gaggtctata aaattataga gaaagttgat tacccccggg atgaaaatgg agaaattgct
      721 gctatcatcc atcctaatct gcaggatcaa gactggaaac cactgcatcc tggggatccc
      781 atgtttttaa ctcttgatgg gaagacgatc ccactgggcg gagactgtac cgtgtacccc
      841 gtgtttgtga atgaggccgc atattacgaa aagaaagaag cttttgcaaa gacaactaaa
      901 ctaacgctca atgcaaaaag tattcgctgc tgtttacat
//