LOCUS CR541903 639 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D1233D for
gene EDN1, endothelin 1; complete cds, incl. stopcodon.
ACCESSION CR541903
VERSION CR541903.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 639)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 639)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D1233D, ORFNo 3906
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1233D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH130879.01X
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence S56805
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..639
/db_xref="H-InvDB:HIT000268773"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D1233D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..639
/codon_start=1
/gene="EDN1"
/db_xref="GOA:Q6FH53"
/db_xref="H-InvDB:HIT000268773.11"
/db_xref="InterPro:IPR001928"
/db_xref="InterPro:IPR019764"
/db_xref="InterPro:IPR020475"
/db_xref="UniProtKB/TrEMBL:Q6FH53"
/protein_id="CAG46701.1"
/translation="MDYLLMIFSLLFVACQGAPETAVLGAELSAVGENGGEKPTPSPP
WRLRRSKRCSCSSLMDKECVYFCHLDIIWVNTPEHVVPYGLGSPRSKRALENLLPTKA
TDRENRCQCASQKDKKCWNFCQAGKELRAEDIMEKDWNNHKKGKDCSKLGKKCIYQQL
VRGRKIRRSSEEHLRQTRSETMRNSVKSSFHDPKLKGKPSRERYVTHNRAHW"
BASE COUNT 192 a 147 c 166 g 134 t
ORIGIN
1 atggattatt tgctcatgat tttctctctg ctgtttgtgg cttgccaagg agctccagaa
61 acagcagtct taggcgctga gctcagcgcg gtgggtgaga acggcgggga gaaacccact
121 cccagtccac cctggcggct ccgccggtcc aagcgctgct cctgctcgtc cctgatggat
181 aaagagtgtg tctacttctg ccacctggac atcatttggg tcaacactcc cgagcacgtt
241 gttccgtatg gacttggaag ccctaggtcc aagagagcct tggagaattt acttcccaca
301 aaggcaacag accgtgagaa tagatgccaa tgtgctagcc aaaaagacaa gaagtgctgg
361 aatttttgcc aagcaggaaa agaactcagg gctgaagaca ttatggagaa agactggaat
421 aatcataaga aaggaaaaga ctgttccaag cttgggaaaa agtgtattta tcagcagtta
481 gtgagaggaa gaaaaatcag aagaagttca gaggaacacc taagacaaac caggtcggag
541 accatgagaa acagcgtcaa atcatctttt catgatccca agctgaaagg caagccctcc
601 agagagcgtt atgtgaccca caaccgagca cattggtga
//