LOCUS CR541900 633 bp mRNA linear HUM 29-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C1133D for
gene NTF5, neurotrophin 5 (neurotrophin 4/5); complete cds, incl.
stopcodon.
ACCESSION CR541900
VERSION CR541900.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 633)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 633)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C1133D, ORFNo 3899
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C1133D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_006179 (GI:34328933)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..633
/db_xref="H-InvDB:HIT000268770"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C1133D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..633
/codon_start=1
/gene="NTF5"
/db_xref="GOA:P34130"
/db_xref="H-InvDB:HIT000268770.13"
/db_xref="HGNC:HGNC:8024"
/db_xref="InterPro:IPR002072"
/db_xref="InterPro:IPR019846"
/db_xref="InterPro:IPR020408"
/db_xref="InterPro:IPR020432"
/db_xref="InterPro:IPR029034"
/db_xref="PDB:1B8M"
/db_xref="PDB:1B98"
/db_xref="PDB:1HCF"
/db_xref="UniProtKB/Swiss-Prot:P34130"
/protein_id="CAG46698.1"
/translation="MLPLPSCSLPILLLFLLPSVPIESQPPPSTLPPFLAPEWDLLSP
RVVLSRGAPAGPPLLFLLEAGAFRESAGAPANRSRRGVSETAPASRRGELAVCDAVSG
WVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRG
VDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA"
BASE COUNT 89 a 204 c 211 g 129 t
ORIGIN
1 atgctccctc tcccctcatg ctccctcccc atcctcctcc ttttcctcct ccccagtgtg
61 ccaattgagt cccaaccccc accctcaaca ttgccccctt ttctggcccc tgagtgggac
121 cttctctccc cccgagtagt cctgtctagg ggtgcccctg ctgggccccc tctgctcttc
181 ctgctggagg ctggggcctt tcgggagtca gcaggtgccc cggccaaccg cagccggcgt
241 ggggtgagcg aaactgcacc agcgagtcgt cggggtgagc tggctgtgtg cgatgcagtc
301 agtggctggg tgacagaccg ccggaccgct gtggacttgc gtgggcgcga ggtggaggtg
361 ttgggcgagg tgcctgcagc tggcggcagt cccctccgcc agtacttctt tgaaacccgc
421 tgcaaggctg ataacgctga ggaaggtggc ccgggggcag gtggaggggg ctgccgggga
481 gtggacagga ggcactgggt atctgagtgc aaggccaagc agtcctatgt gcgggcattg
541 accgctgatg cccagggccg tgtgggctgg cgatggattc gaattgacac tgcctgcgtc
601 tgcacactcc tcagccggac tggccgggcc tga
//