LOCUS CR541897 1260 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C1033D for
gene VEGFC, vascular endothelial growth factor C; complete cds,
incl. stopcodon.
ACCESSION CR541897
VERSION CR541897.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1260)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1260)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C1033D, ORFNo 3888
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C1033D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_005429 (GI:19924300)
we found AA exchange(s) at position (first base of changed
triplet):
1078(cys->tyr)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1260
/db_xref="H-InvDB:HIT000268767"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C1033D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1260
/codon_start=1
/gene="VEGFC"
/db_xref="GOA:Q6FH59"
/db_xref="H-InvDB:HIT000268767.13"
/db_xref="InterPro:IPR000072"
/db_xref="InterPro:IPR004153"
/db_xref="InterPro:IPR023581"
/db_xref="InterPro:IPR029034"
/db_xref="UniProtKB/TrEMBL:Q6FH59"
/protein_id="CAG46695.1"
/translation="MHLLGFFSVACSLLAAALLPGPREAPAAAAAFESGLDLSDAEPD
AGEATAYASKDLEEQLRSVSSVDELMTVLYPEYWKMYKCQLRKGGWQHNREQANLNSR
TEETIKFAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFFKPPCVSVY
RCGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVY
RQVHSIIRRSLPATLPQCQAANKTCPTNYMWNNHICRCLAQEDFMFSSDAGDDSTDGF
HDICGPNKELDEETCQCVCRAGLRPASCGPHKELDRNSCQCVCKNKLFPSQCGANREF
DENTCQCVCKRTCPRNQPLNPGKCAYECTESPQKCLLKGKKFHHQTCSCYRRPCTNRQ
KACEPGFSYSEEVCRCVPSYWKRPQMS"
BASE COUNT 360 a 309 c 312 g 279 t
ORIGIN
1 atgcacttgc tgggcttctt ctctgtggcg tgttctctgc tcgccgctgc gctgctcccg
61 ggtcctcgcg aggcgcccgc cgccgccgcc gccttcgagt ccggactcga cctctcggac
121 gcggagcccg acgcgggcga ggccacggct tatgcaagca aagatctgga ggagcagtta
181 cggtctgtgt ccagtgtaga tgaactcatg actgtactct acccagaata ttggaaaatg
241 tacaagtgtc agctaaggaa aggaggctgg caacataaca gagaacaggc caacctcaac
301 tcaaggacag aagagactat aaaatttgct gcagcacatt ataatacaga gatcttgaaa
361 agtattgata atgagtggag aaagactcaa tgcatgccac gggaggtgtg tatagatgtg
421 gggaaggagt ttggagtcgc gacaaacacc ttctttaaac ctccatgtgt gtccgtctac
481 agatgtgggg gttgctgcaa tagtgagggg ctgcagtgca tgaacaccag cacgagctac
541 ctcagcaaga cgttatttga aattacagtg cctctctctc aaggccccaa accagtaaca
601 atcagttttg ccaatcacac ttcctgccga tgcatgtcta aactggatgt ttacagacaa
661 gttcattcca ttattagacg ttccctgcca gcaacactac cacagtgtca ggcagcgaac
721 aagacctgcc ccaccaatta catgtggaat aatcacatct gcagatgcct ggctcaggaa
781 gattttatgt tttcctcgga tgctggagat gactcaacag atggattcca tgacatctgt
841 ggaccaaaca aggagctgga tgaagagacc tgtcagtgtg tctgcagagc ggggcttcgg
901 cctgccagct gtggacccca caaagaacta gacagaaact catgccagtg tgtctgtaaa
961 aacaaactct tccccagcca atgtggggcc aaccgagaat ttgatgaaaa cacatgccag
1021 tgtgtatgta aaagaacctg ccccagaaat caacccctaa atcctggaaa atgtgcctat
1081 gaatgtacag aaagtccaca gaaatgcttg ttaaaaggaa agaagttcca ccaccaaaca
1141 tgcagctgtt acagacggcc atgtacgaac cgccagaagg cttgtgagcc aggattttca
1201 tatagtgaag aagtgtgtcg ttgtgtccct tcatattgga aaagaccaca aatgagctaa
//