LOCUS       CR541886                 507 bp    mRNA    linear   HUM 29-JUN-2004
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834B0633D for
            gene MDS1, myelodysplasia syndrome 1; complete cds, without
            stopcodon.
ACCESSION   CR541886
VERSION     CR541886.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 507)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  JOURNAL   Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834B0633D, ORFNo 3866
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0633D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            Contact RZPD (customer.service@rzpd.de) for further information.
            Clone name at Harvard Institute of Proteomics
            (www.hip.harvard.edu):
            FLH130857.01L
            
            This CDS clone is part of a collection of human full ORF clones
            jointly established and verified by the Harvard Institute of
            Proteomics (HIP) and RZPD.
            This CDS has been cloned without stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC TCC ACC (ATG).
            The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
            The clone is validated by full sequence check.
            Compared to the reference sequence NM_004991 (GI:4826827)
            we did not find any amino acid exchanges.
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..507
                     /db_xref="H-InvDB:HIT000268756"
                     /organism="Homo sapiens"
                     /lab_host="DH5Alpha"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834B0633D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..>507
                     /codon_start=1
                     /gene="MDS1"
                     /db_xref="GOA:Q03112"
                     /db_xref="H-InvDB:HIT000268756.11"
                     /db_xref="HGNC:HGNC:3498"
                     /db_xref="InterPro:IPR001214"
                     /db_xref="InterPro:IPR013087"
                     /db_xref="InterPro:IPR036236"
                     /db_xref="PDB:6BW3"
                     /db_xref="UniProtKB/Swiss-Prot:Q03112"
                     /protein_id="CAG46684.1"
                     /translation="MRSKGRARKLATNNECVYGNYPEIPLEEMPDADGVASTPSLNIQ
                     EPCSPATSSEAFTPKEGSPYKAPIYIPDDIPIPAEFELRESNMPGAGLGIWTKRKIEV
                     GEKFGPYVGEQRSNLKDPSYGWEVHLPRSRRVSVHSWLYLGKRSSDVGIAFSQADVYM
                     PGLQCAFLS"
BASE COUNT          142 a          118 c          130 g          117 t
ORIGIN      
        1 atgagatcca aaggcagggc aaggaaactg gccacaaata atgagtgtgt atatggcaac
       61 taccctgaaa tacctttgga agaaatgcca gatgcagatg gagtagccag cactccctcc
      121 ctcaatattc aagagccatg ctctcctgcc acatccagtg aagcattcac tccaaaggag
      181 ggttctcctt acaaagcccc catctacatc cctgatgata tccccattcc tgctgagttt
      241 gaacttcgag agtcaaatat gcctggggca ggactaggaa tatggaccaa aaggaagatc
      301 gaagtaggtg aaaagtttgg gccttatgtg ggagagcaga ggtcaaacct gaaagacccc
      361 agttatggat gggaggtaca tcttccaagg tctcggaggg taagcgttca ctcttggttg
      421 tatttgggga agagaagctc agacgtagga atagccttct ctcaggctga tgtctacatg
      481 cctggactgc agtgtgcctt cctctcg
//