LOCUS CR541882 531 bp mRNA linear HUM 29-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0533D for
gene PTHLH, parathyroid hormone-like hormone; complete cds, without
stopcodon.
ACCESSION CR541882
VERSION CR541882.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 531)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 531)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0533D, ORFNo 3862
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0533D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_198966 (GI:39995092)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..531
/db_xref="H-InvDB:HIT000268752"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0533D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>531
/codon_start=1
/gene="PTHLH"
/db_xref="GOA:P12272"
/db_xref="H-InvDB:HIT000268752.11"
/db_xref="HGNC:HGNC:9607"
/db_xref="InterPro:IPR001415"
/db_xref="InterPro:IPR003626"
/db_xref="PDB:1BZG"
/db_xref="PDB:1ET3"
/db_xref="PDB:1M5N"
/db_xref="PDB:3FFD"
/db_xref="PDB:3H3G"
/db_xref="UniProtKB/Swiss-Prot:P12272"
/protein_id="CAG46680.1"
/translation="MQRRLVQQWSVAVFLLSYAVPSCGRSVEGLSRRLKRAVSEHQLL
HDKGKSIQDLRRRFFLHHLIAEIHTAEIRATSEVSPNSKPSPNTKNHPVRFGSDDEGR
YLTQETNKVETYKEQPLKTPGKKKKGKPGKRKEQEKKKRRTRSAWLDSGVTGSGLEGD
HLSDTSTTSLELDSRRH"
BASE COUNT 143 a 148 c 149 g 91 t
ORIGIN
1 atgcagcgga gactggttca gcagtggagc gtcgcggtgt tcctgctgag ctacgcggtg
61 ccctcctgcg ggcgctcggt ggagggtctc agccgccgcc tcaaaagagc tgtgtctgaa
121 catcagctcc tccatgacaa ggggaagtcc atccaagatt tacggcgacg attcttcctt
181 caccatctga tcgcagaaat ccacacagct gaaatcagag ctacctcgga ggtgtcccct
241 aactccaagc cctctcccaa cacaaagaac caccccgtcc gatttgggtc tgatgatgag
301 ggcagatacc taactcagga aactaacaag gtggagacgt acaaagagca gccgctcaag
361 acacctggga agaaaaagaa aggcaagccc gggaaacgca aggagcagga aaagaaaaaa
421 cggcgaactc gctctgcctg gttagactct ggagtgactg ggagtgggct agaaggggac
481 cacctgtctg acacctccac aacgtcgctg gagctcgatt cacggaggca t
//